Rabbit Recombinant Monoclonal CD13 antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-CD13 antibody [EPR4058] - BSA and Azide free (AB271872), expandable thumbnail](https://content.abcam.com/products/images/cd13-antibody-epr4058-bsa-and-azide-free-ab271872--immunocytochemistry-immunofluorescence-img53227.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CD13 antibody [EPR4058] - BSA and Azide free (AB271872)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (AB271872), expandable thumbnail](https://content.abcam.com/products/images/cd13-antibody-epr4058-bsa-and-azide-free-ab271872--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img95483.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (AB271872)")
![Western blot - Anti-CD13 antibody [EPR4058] - BSA and Azide free (AB271872), expandable thumbnail](https://content.abcam.com/products/images/cd13-antibody-epr4058-bsa-and-azide-free-ab271872--western-blot-img101557.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CD13 antibody [EPR4058] - BSA and Azide free (AB271872)")
![Immunocytochemistry/ Immunofluorescence - Anti-CD13 antibody [EPR4058] - BSA and Azide free (AB271872), expandable thumbnail](https://content.abcam.com/products/images/cd13-antibody-epr4058-bsa-and-azide-free-ab271872--immunocytochemistry-immunofluorescence-img95276.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CD13 antibody [EPR4058] - BSA and Azide free (AB271872)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (AB271872), expandable thumbnail](https://content.abcam.com/products/images/cd13-antibody-epr4058-bsa-and-azide-free-ab271872--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img105370.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (AB271872)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Tested | Tested |
| Mouse | Not recommended | Not recommended | Tested | Not recommended | Expected |
| Rat | Not recommended | Not recommended | Expected | Tested | Expected |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
2 products for Alternative Version
Target data
Function
Broad specificity aminopeptidase which plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Also involved in the processing of various peptides including peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. May also be involved the cleavage of peptides bound to major histocompatibility complex class II molecules of antigen presenting cells. May have a role in angiogenesis and promote cholesterol crystallization. May have a role in amino acid transport by acting as binding partner of amino acid transporter SLC6A19 and regulating its activity (By similarity). (Microbial infection) Acts as a receptor for human coronavirus 229E/HCoV-229E. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. (Microbial infection) Mediates as well Human cytomegalovirus (HCMV) infection.
Alternative names
CD13, APN, PEPN, ANPEP, Aminopeptidase N, AP-N, hAPN, Alanyl aminopeptidase, Aminopeptidase M, Microsomal aminopeptidase, Myeloid plasma membrane glycoprotein CD13, gp150, AP-M
Recommended products
Rabbit Recombinant Monoclonal CD13 antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR4058
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab271872 is the carrier-free version of Anti-CD13 antibody [EPR4058] ab108310.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD13 also known as aminopeptidase N (ANPEP) is a transmembrane protein with a molecular weight of approximately 150 kDa. It functions as a zinc-dependent metalloenzyme which cleaves N-terminal amino acids from peptides and proteins. CD13 protein is expressed on various cell types including myeloid cells epithelial cells endothelial cells and fibroblasts. Its presence is significantly observed in the brush border of the small intestinal mucosa and the renal proximal tubule. Researchers can study CD13 using anti-CD13 antibodies and CD13 ELISA kits.
- Biological function summary
CD13 regulates peptide-mediated signaling and controls the maturation and catabolism of bioactive peptides. The enzymatic function of CD13 influences processes such as cell proliferation motility and angiogenesis. It does not operate as part of a larger complex but its activity modulates several cellular and systemic functions. CD13's role in these biological processes highlights its importance in modulating local and systemic peptide pools which contributes to its diverse physiological effects.
- Pathways
CD13 plays significant roles in the renin-angiotensin system and the regulation of inflammatory responses. In the renin-angiotensin system CD13 modulates the activity of angiotensin influencing blood pressure and fluid balance. Through its enzymatic activity CD13 interacts with proteins such as ACE another critical player in this pathway. In inflammation CD13 regulates the availability of chemotactic peptides affecting leukocyte migration and adhesion. The proteins it works with in inflammatory pathways include cytokines which CD13 indirectly modulates by altering chemokine activity.
- Associated diseases and disorders
Aberrant CD13 expression and activity are implicated in cancer and inflammatory diseases. In cancer overexpression of CD13 correlates with tumor growth and metastasis particularly in cancers like renal cell carcinoma and prostate cancer. It can confer an increased malignant phenotype by promoting angiogenesis and immune evasion. In inflammatory disorders dysregulation of CD13 exacerbates diseases such as rheumatoid arthritis through its impact on peptide-mediated signaling and immune cell movement. Its connection with inflammatory cytokines like IL-8 indicates its important role in mediating inflammatory responses.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
16 product images
![Immunocytochemistry/ Immunofluorescence - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872), expandable thumbnail](https://content.abcam.com/products/images/cd13-antibody-epr4058-bsa-and-azide-free-ab271872--immunocytochemistry-immunofluorescence-img53227.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872)")
Immunocytochemistry/ Immunofluorescence - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872)
This data was developed using the same antibody clone in a different buffer formulation (Anti-CD13 antibody [EPR4058] ab108310). Anti-CD13 antibody [EPR4058] ab108310 staining CD13 in wild-type THP-1 cells (top panel) and ANPEP knockout THP-1 cells (bottom panel) (Human ANPEP (CD13) knockout THP-1 cell line ab273759). The cells were fixed with 4% paraformaldehyde (10 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with Anti-CD13 antibody [EPR4058] ab108310 at 1/500 dilution and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872), expandable thumbnail](https://content.abcam.com/products/images/cd13-antibody-epr4058-bsa-and-azide-free-ab271872--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img95483.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling CD13 with purified Anti-CD13 antibody [EPR4058] ab108310 at 1/1600 dilution (0.43 µg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD13 antibody [EPR4058] ab108310).
![Western blot - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872), expandable thumbnail](https://content.abcam.com/products/images/cd13-antibody-epr4058-bsa-and-azide-free-ab271872--western-blot-img101557.jpg "Western blot - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872)")
Western blot - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872)
This data was developed using the same antibody clone in a different buffer formulation (Anti-CD13 antibody [EPR4058] ab108310).
Lanes 1 - 4: Merged signal (red and green). Green - Anti-CD13 antibody [EPR4058] ab108310 observed at 160 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
Anti-CD13 antibody [EPR4058] ab108310 was shown to react with CD13 in wild-type THP-1 cells in western blot with loss of signal observed in ANPEP knockout cell line Human ANPEP (CD13) knockout THP-1 cell line ab273759 (knockout cell lysate Human ANPEP (CD13) knockout THP-1 cell lysate ab275505). Wild-type and ANPEP knockout THP-1 cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with Anti-CD13 antibody [EPR4058] ab108310 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-CD13 antibody [EPR4058] (Anti-CD13 antibody [EPR4058] ab108310) at 1/1000 dilution
Lane 1: Wild-type THP-1 cell lysate at 30 µg
Lane 2: ANPEP knockout THP-1 cell lysate at 30 µg
Lane 2: Western blot - Human ANPEP (CD13) knockout THP-1 cell line (Human ANPEP (CD13) knockout THP-1 cell line ab273759)
Lane 3: PANC-1 cell lysate at 30 µg
Lane 4: HEK-293 cell lysate at 30 µg
Performed under reducing conditions.
Predicted band size: 109 kDa
Observed band size: 160 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872), expandable thumbnail](https://content.abcam.com/products/images/cd13-antibody-epr4058-bsa-and-azide-free-ab271872--immunocytochemistry-immunofluorescence-img95276.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872)")
Immunocytochemistry/ Immunofluorescence - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872)
Confocal image showing membranous staining in THP-1 cells
Anti-CD13 antibody [EPR4058] ab108310 (purified) at 1/100 staining CD13 in the THP-1 (human monocytic leukemia monocyte) cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol. Samples were incubated with primary antibody 1/500. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 An Alexa Fluor® 488-conjugated Goat anti-Rabbit IgG at 1/1000 was used as the secondary antibody. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)1/200 was used as a counter stain and DAPI was used as a nuclear counter stain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD13 antibody [EPR4058] ab108310).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872), expandable thumbnail](https://content.abcam.com/products/images/cd13-antibody-epr4058-bsa-and-azide-free-ab271872--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img105370.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling CD13 with purified Anti-CD13 antibody [EPR4058] ab108310 at 1/1600 dilution (0.43 µg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD13 antibody [EPR4058] ab108310).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872), expandable thumbnail](https://content.abcam.com/products/images/cd13-antibody-epr4058-bsa-and-azide-free-ab271872--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img139087.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872)
Anti-CD13 antibody [EPR4058] ab108310 (unpurified), at 1/250, staining CD13 in human kidney tissue by immunohistochemistry. Heat mediated antigen retrieval was performed using citrate buffer pH 6 before commencing with IHC staining protocol.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD13 antibody [EPR4058] ab108310).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872), expandable thumbnail](https://content.abcam.com/products/images/cd13-antibody-epr4058-bsa-and-azide-free-ab271872--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133725.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872)
Anti-CD13 antibody [EPR4058] ab108310 (unpurified) showing positive staining in human normal liver tissue. Heat mediated antigen retrieval was performed using citrate buffer pH 6 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD13 antibody [EPR4058] ab108310).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872), expandable thumbnail](https://content.abcam.com/products/images/cd13-antibody-epr4058-bsa-and-azide-free-ab271872--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img90673.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872)
Anti-CD13 antibody [EPR4058] ab108310 (unpurified) showing positive staining in human hepatocellular carcinoma tissue. Heat mediated antigen retrieval was performed using citrate buffer pH 6 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD13 antibody [EPR4058] ab108310).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872), expandable thumbnail](https://content.abcam.com/products/images/cd13-antibody-epr4058-bsa-and-azide-free-ab271872--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img81765.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872)
Anti-CD13 antibody [EPR4058] ab108310 (unpurified) showing positive staining in human astrocytoma tissue. Heat mediated antigen retrieval was performed using citrate buffer pH 6 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD13 antibody [EPR4058] ab108310).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872), expandable thumbnail](https://content.abcam.com/products/images/cd13-antibody-epr4058-bsa-and-azide-free-ab271872--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img36829.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872)
Anti-CD13 antibody [EPR4058] ab108310 (unpurified) showing positive staining in human normal breast tissue. Heat mediated antigen retrieval was performed using citrate buffer pH 6 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD13 antibody [EPR4058] ab108310).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872), expandable thumbnail](https://content.abcam.com/products/images/cd13-antibody-epr4058-bsa-and-azide-free-ab271872--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img113278.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872)
Anti-CD13 antibody [EPR4058] ab108310 (unpurified) showing positive staining in human prostatic carcinoma tissue. Heat mediated antigen retrieval was performed using citrate buffer pH 6 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD13 antibody [EPR4058] ab108310).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872), expandable thumbnail](https://content.abcam.com/products/images/cd13-antibody-epr4058-bsa-and-azide-free-ab271872--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img129285.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872)
Anti-CD13 antibody [EPR4058] ab108310 (unpurified) showing positive staining in human normal tonsil tissue. Heat mediated antigen retrieval was performed using citrate buffer pH 6 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD13 antibody [EPR4058] ab108310).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872), expandable thumbnail](https://content.abcam.com/products/images/cd13-antibody-epr4058-bsa-and-azide-free-ab271872--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133970.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872)
Anti-CD13 antibody [EPR4058] ab108310 (unpurified) showing positive staining in human normal stomach tissue. Heat mediated antigen retrieval was performed using citrate buffer pH 6 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD13 antibody [EPR4058] ab108310).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872), expandable thumbnail](https://content.abcam.com/products/images/cd13-antibody-epr4058-bsa-and-azide-free-ab271872--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img3735.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872)
Anti-CD13 antibody [EPR4058] ab108310 (unpurified) showing positive staining in human normal colon tissue. Heat mediated antigen retrieval was performed using citrate buffer pH 6 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD13 antibody [EPR4058] ab108310).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872), expandable thumbnail](https://content.abcam.com/products/images/cd13-antibody-epr4058-bsa-and-azide-free-ab271872--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img90066.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872)
Anti-CD13 antibody [EPR4058] ab108310 (unpurified) showing positive staining in human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed using citrate buffer pH 6 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD13 antibody [EPR4058] ab108310).
![Western blot - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872), expandable thumbnail](https://content.abcam.com/products/images/cd13-antibody-epr4058-bsa-and-azide-free-ab271872--western-blot-img444559.jpg "Western blot - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872)")
Western blot - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872)
CD13 Western blot staining using rabbit Anti-CD13 antibody
This data was developed using Anti-CD13 antibody [EPR4058] ab108310, the same antibody clone in a different buffer formulation.
The identity of the lower MW band at approximately 75 kDa is non-specific banding.
The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Anpep/CD13-KO homozygous mice (Strain ID: T027669).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-CD13 antibody [EPR4058] (Anti-CD13 antibody [EPR4058] ab108310) at 1/1000 dilution
Lane 1: Wild-type mouse liver tissue lysate (male)
Lanes 2 - 3: Anpep/CD13 knockout liver tissue lysate (male)
Lane 4: Wild-type mouse kidney tissue lysate (male)
Lanes 5 - 6: Anpep/CD13 knockout kidney tissue lysate (male)
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 150 kDa
Exposure time: 38s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872), expandable thumbnail](https://content.abcam.com/products/images/cd13-antibody-epr4058-bsa-and-azide-free-ab271872--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img139087.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872), expandable thumbnail](https://content.abcam.com/products/images/cd13-antibody-epr4058-bsa-and-azide-free-ab271872--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133725.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872), expandable thumbnail](https://content.abcam.com/products/images/cd13-antibody-epr4058-bsa-and-azide-free-ab271872--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img90673.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872), expandable thumbnail](https://content.abcam.com/products/images/cd13-antibody-epr4058-bsa-and-azide-free-ab271872--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img81765.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872), expandable thumbnail](https://content.abcam.com/products/images/cd13-antibody-epr4058-bsa-and-azide-free-ab271872--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img36829.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872), expandable thumbnail](https://content.abcam.com/products/images/cd13-antibody-epr4058-bsa-and-azide-free-ab271872--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img113278.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872), expandable thumbnail](https://content.abcam.com/products/images/cd13-antibody-epr4058-bsa-and-azide-free-ab271872--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img129285.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872), expandable thumbnail](https://content.abcam.com/products/images/cd13-antibody-epr4058-bsa-and-azide-free-ab271872--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133970.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872), expandable thumbnail](https://content.abcam.com/products/images/cd13-antibody-epr4058-bsa-and-azide-free-ab271872--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img3735.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872), expandable thumbnail](https://content.abcam.com/products/images/cd13-antibody-epr4058-bsa-and-azide-free-ab271872--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img90066.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872)")
![Western blot - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872), expandable thumbnail](https://content.abcam.com/products/images/cd13-antibody-epr4058-bsa-and-azide-free-ab271872--western-blot-img444559.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872)")
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