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AB33489

Anti-CD13 antibody [R3-63]

4

(2 Reviews)

|

(8 Publications)

Rat Monoclonal CD13 antibody. Suitable for Flow Cyt, IHC-Fr, IHC-P and reacts with Mouse samples. Cited in 8 publications.

View Alternative Names

CD13, Lap-1, Lap1, Anpep, Aminopeptidase N, AP-N, mAPN, Alanyl aminopeptidase, Aminopeptidase M, Membrane protein p161, Microsomal aminopeptidase, AP-M

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [R3-63] (AB33489)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [R3-63] (AB33489)

Immunofluorescence ab33489, green in A and a Rat anti Mouse CD8, clone YTS105.18, red in B. C is the merged image with nuclei counterstained blue using DAPI. Medium power

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [R3-63] (AB33489)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [R3-63] (AB33489)

Immunofluorescence staining of a mouse lymph node cryosection with ab33489, green in A and a Rat anti Mouse CD8, clone YTS105.18, red in B. C is the merged image with nuclei counterstained blue using DAPI. High power

Immunohistochemistry (Frozen sections) - Anti-CD13 antibody [R3-63] (AB33489)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-CD13 antibody [R3-63] (AB33489)

Immunoperoxidase staining of mouse lymph node cryosection with ab33489 followed by a horseradish peroxidase Goat anti Rat IgG antibody. High power

Immunohistochemistry (Frozen sections) - Anti-CD13 antibody [R3-63] (AB33489)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-CD13 antibody [R3-63] (AB33489)

Immunoperoxidase staining of mouse lymph node cryosection with ab33489 followed by a horseradish peroxidase Goat anti Rat IgG antibody. Medium power

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [R3-63] (AB33489)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [R3-63] (AB33489)

Immunofluorescence staining of a mouse lymph node cryosection with ab33489, green in A and a Rat monoclonal anti Mouse CD8, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue using DAPI. Low power

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

R3-63

Isotype

IgG2a

Carrier free

No

Reacts with

Mouse

Applications

IHC-P, IHC-Fr, Flow Cyt

applications

Specificity

ab33489 recognizes mouse aminopeptidase N (APN), a cell surface protein homologous with human CD13.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>Use 10ul of the suggested working dilution to label 10^6 cells. <a href='/en-us/products/primary-antibodies/rat-igg2a-kappa-monoclonal-rtk2758-isotype-control-ab18450'>ab18450</a> - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Clone R3-63 has been reported to block mouse APN enzyme activity.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified IgG prepared by affinity chromatography on Protein G.
Storage buffer
pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD13 also known as aminopeptidase N (ANPEP) is a transmembrane protein with a molecular weight of approximately 150 kDa. It functions as a zinc-dependent metalloenzyme which cleaves N-terminal amino acids from peptides and proteins. CD13 protein is expressed on various cell types including myeloid cells epithelial cells endothelial cells and fibroblasts. Its presence is significantly observed in the brush border of the small intestinal mucosa and the renal proximal tubule. Researchers can study CD13 using anti-CD13 antibodies and CD13 ELISA kits.
Biological function summary

CD13 regulates peptide-mediated signaling and controls the maturation and catabolism of bioactive peptides. The enzymatic function of CD13 influences processes such as cell proliferation motility and angiogenesis. It does not operate as part of a larger complex but its activity modulates several cellular and systemic functions. CD13's role in these biological processes highlights its importance in modulating local and systemic peptide pools which contributes to its diverse physiological effects.

Pathways

CD13 plays significant roles in the renin-angiotensin system and the regulation of inflammatory responses. In the renin-angiotensin system CD13 modulates the activity of angiotensin influencing blood pressure and fluid balance. Through its enzymatic activity CD13 interacts with proteins such as ACE another critical player in this pathway. In inflammation CD13 regulates the availability of chemotactic peptides affecting leukocyte migration and adhesion. The proteins it works with in inflammatory pathways include cytokines which CD13 indirectly modulates by altering chemokine activity.

Aberrant CD13 expression and activity are implicated in cancer and inflammatory diseases. In cancer overexpression of CD13 correlates with tumor growth and metastasis particularly in cancers like renal cell carcinoma and prostate cancer. It can confer an increased malignant phenotype by promoting angiogenesis and immune evasion. In inflammatory disorders dysregulation of CD13 exacerbates diseases such as rheumatoid arthritis through its impact on peptide-mediated signaling and immune cell movement. Its connection with inflammatory cytokines like IL-8 indicates its important role in mediating inflammatory responses.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Broad specificity aminopeptidase which plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Also involved in the processing of various peptides including peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines (By similarity). May also be involved the cleavage of peptides bound to major histocompatibility complex class II molecules of antigen presenting cells (PubMed : 8691132). May have a role in angiogenesis and promote cholesterol crystallization (By similarity). May have a role in amino acid transport by acting as binding partner of amino acid transporter SLC6A19 and regulating its activity (PubMed : 22677001).
See full target information Anpep
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Science (New York, N.Y.) 372:1205-1209 PubMed34112692

2021

Release of stem cells from quiescence reveals gliogenic domains in the adult mouse brain.

Applications

Unspecified application

Species

Unspecified reactive species

Ana C Delgado,Angel R Maldonado-Soto,Violeta Silva-Vargas,Dogukan Mizrak,Thomas von Känel,Kelly R Tan,Alex Paul,Aviv Madar,Henar Cuervo,Jan Kitajewski,Chyuan-Sheng Lin,Fiona Doetsch

Environmental pollution (Barking, Essex : 1987) 278:116755 PubMed33725534

2021

Varying modalities of perinatal exposure to a pesticide cocktail elicit neurological adaptations in mice and zebrafish.

Applications

Unspecified application

Species

Unspecified reactive species

Isabel Forner-Piquer,Wendy Klement,Giuseppe Gangarossa,Emma Zub,Frederic de Bock,Marine Blaquiere,Tangui Maurice,Etienne Audinat,Adèle Faucherre,Frederic Lasserre,Sandrine Ellero-Simatos,Laurence Gamet-Payrastre,Chris Jopling,Nicola Marchi

Molecular neurobiology 58:1392-1403 PubMed33184784

2020

Reactive Fibroblasts in Response to Optic Nerve Crush Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangxiang Liu,Yuan Liu,Huiyi Jin,Mohamed M Khodeiry,Weizheng Kong,Ningli Wang,Jae K Lee,Richard K Lee

Biomedical microdevices 22:34 PubMed32377802

2020

Biomimetic aorta-gonad-Mesonephros-on-a-Chip to study human developmental hematopoiesis.

Applications

Unspecified application

Species

Unspecified reactive species

Ryohichi Sugimura,Ryo Ohta,Chihiro Mori,Alina Li,Takafumi Mano,Emi Sano,Kaori Kosugi,Tatsutoshi Nakahata,Akira Niwa,Megumu K Saito,Yu-Suke Torisawa

Scientific reports 9:16965 PubMed31740720

2019

Elemental Zn and its Binding Protein Zinc-α2-Glycoprotein are Elevated in HPV-Positive Oropharyngeal Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Kate Poropatich,Tatjana Paunesku,Alia Zander,Brian Wray,Matthew Schipma,Prarthana Dalal,Mark Agulnik,Si Chen,Barry Lai,Olga Antipova,Evan Maxey,Koshonna Brown,Michael Beau Wanzer,Demirkan Gursel,Hanli Fan,Alfred Rademaker,Gayle E Woloschak,Bharat B Mittal

Disease models & mechanisms 11: PubMed29622551

2018

Choroidal pericytes promote subretinal fibrosis after experimental photocoagulation.

Applications

Unspecified application

Species

Unspecified reactive species

Xueting Luo,Shiqi Yang,Jian Liang,Yuanqi Zhai,Mengxi Shen,Junran Sun,Yiji Feng,Xinmin Lu,Hong Zhu,Fenghua Wang,Xiaodong Sun

The Journal of neuroscience : the official journal of the Society for Neuroscience 35:4528-39 PubMed25788671

2015

Regional and stage-specific effects of prospectively purified vascular cells on the adult V-SVZ neural stem cell lineage.

Applications

Unspecified application

Species

Unspecified reactive species

Elizabeth E Crouch,Chang Liu,Violeta Silva-Vargas,Fiona Doetsch

European journal of immunology 23:2358-64 PubMed8103749

1993

A mouse aminopeptidase N is a marker for antigen-presenting cells and appears to be co-expressed with major histocompatibility complex class II molecules.

Applications

Unspecified application

Species

Unspecified reactive species

A S Hansen,O Norén,H Sjöström,O Werdelin
View all publications
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