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Mouse Recombinant Monoclonal CD13 antibody. Suitable for Flow Cyt, ICC/IF and reacts with Human samples. Cited in 21 publications.


Images

Flow Cytometry - Anti-CD13 antibody [WM15] (AB7417), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CD13 antibody [WM15] (AB7417), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CD13 antibody [WM15] (AB7417), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CD13 antibody [WM15] (AB7417), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytICC/IF
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1 µg/mL
Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species
Human
Dilution info
1 µg/mL
Notes

This product also produced a positive signal in A375 when fixed with 4% formaldehyde (10min)

Associated Products

Select an associated product type

7 products for Alternative Version

7 products for Alternative Product

Target data

Function

Broad specificity aminopeptidase which plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Also involved in the processing of various peptides including peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. May also be involved the cleavage of peptides bound to major histocompatibility complex class II molecules of antigen presenting cells. May have a role in angiogenesis and promote cholesterol crystallization. May have a role in amino acid transport by acting as binding partner of amino acid transporter SLC6A19 and regulating its activity (By similarity). (Microbial infection) Acts as a receptor for human coronavirus 229E/HCoV-229E. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. (Microbial infection) Mediates as well Human cytomegalovirus (HCMV) infection.

Alternative names

CD13, APN, PEPN, ANPEP, Aminopeptidase N, AP-N, hAPN, Alanyl aminopeptidase, Aminopeptidase M, Microsomal aminopeptidase, Myeloid plasma membrane glycoprotein CD13, gp150, AP-M

Recommended products

Mouse Recombinant Monoclonal CD13 antibody. Suitable for Flow Cyt, ICC/IF and reacts with Human samples. Cited in 21 publications.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Clone number
WM15
Purification technique
Affinity purification Protein A
Epitope
The antibody recognizes an extracellular epitope.
Concentration
Loading...
Purification notes

Purified by protein-A affinity chromatography> 95% pure (by SDS-PAGE).

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product has switched fom a hybridoma to recombinant production method on 08th March 2021.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CD13 also known as aminopeptidase N (ANPEP) is a transmembrane protein with a molecular weight of approximately 150 kDa. It functions as a zinc-dependent metalloenzyme which cleaves N-terminal amino acids from peptides and proteins. CD13 protein is expressed on various cell types including myeloid cells epithelial cells endothelial cells and fibroblasts. Its presence is significantly observed in the brush border of the small intestinal mucosa and the renal proximal tubule. Researchers can study CD13 using anti-CD13 antibodies and CD13 ELISA kits.

Biological function summary

CD13 regulates peptide-mediated signaling and controls the maturation and catabolism of bioactive peptides. The enzymatic function of CD13 influences processes such as cell proliferation motility and angiogenesis. It does not operate as part of a larger complex but its activity modulates several cellular and systemic functions. CD13's role in these biological processes highlights its importance in modulating local and systemic peptide pools which contributes to its diverse physiological effects.

Pathways

CD13 plays significant roles in the renin-angiotensin system and the regulation of inflammatory responses. In the renin-angiotensin system CD13 modulates the activity of angiotensin influencing blood pressure and fluid balance. Through its enzymatic activity CD13 interacts with proteins such as ACE another critical player in this pathway. In inflammation CD13 regulates the availability of chemotactic peptides affecting leukocyte migration and adhesion. The proteins it works with in inflammatory pathways include cytokines which CD13 indirectly modulates by altering chemokine activity.

Associated diseases and disorders

Aberrant CD13 expression and activity are implicated in cancer and inflammatory diseases. In cancer overexpression of CD13 correlates with tumor growth and metastasis particularly in cancers like renal cell carcinoma and prostate cancer. It can confer an increased malignant phenotype by promoting angiogenesis and immune evasion. In inflammatory disorders dysregulation of CD13 exacerbates diseases such as rheumatoid arthritis through its impact on peptide-mediated signaling and immune cell movement. Its connection with inflammatory cytokines like IL-8 indicates its important role in mediating inflammatory responses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Flow Cytometry - Anti-CD13 antibody [WM15] (ab7417), expandable thumbnail
    This image is courtesy of an anonymous customer review

    Flow Cytometry - Anti-CD13 antibody [WM15] (ab7417)

    This image was generated from the hybridoma version of the product.

    ab7417 staining CD13 in Human Fibrosarcoma cells by flow cytometry. Cells were fixed with paraformaldehyde. The sample was incubated with primary antibody at 10μg/ml in PBS for 1 hour at 20°C. An Allophycocyanin (APC) conjugated anti-mouse monoclonal was used as a secondary antibody at 5μg/ml. Secondary antibody only (red). ab7417 and secondary antibody (blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-CD13 antibody [WM15] (ab7417), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CD13 antibody [WM15] (ab7417)

    ab7417 staining CD13 in PANC-1 cells (top panel - positive control) and HEK-293 cells (bottom panel - negative control). The cells were fixed with 4% paraformaldehyde (10 min) then permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab7417 at 0.5μg/ml concentration and Anti-beta Tubulin antibody - Loading Control ab6046 (Rabbit polyclonal to beta Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to mouse IgG (Alexa Fluor® 488) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117) at 2 μg/ml (shown in green) and a goat secondary antibody to rabbit IgG (Alexa Fluor® 594) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
    Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

  • Immunocytochemistry/ Immunofluorescence - Anti-CD13 antibody [WM15] (ab7417), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CD13 antibody [WM15] (ab7417)

    ab7417 staining CD13 in A375 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°;C with ab7417 at 1μg/ml and Anti-beta Tubulin antibody - Loading Control ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor®; 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

    Also suitable in cells fixed with 4% paraformaldehyde (10 min).

    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown

  • Immunocytochemistry/ Immunofluorescence - Anti-CD13 antibody [WM15] (ab7417), expandable thumbnail
    This image is courtesy of an anonymous customer review

    Immunocytochemistry/ Immunofluorescence - Anti-CD13 antibody [WM15] (ab7417)

    This image was generated from the hybridoma version of the product.

    ab7417 staining CD13 in HT1080 cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at 10μg/ml for 1 hour. A Texas Red Goat Anti-mouse polyclonal was used as the secondary antibody.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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