AB234105

Anti-CD130 (gp130) antibody [EPR21732] - BSA and Azide free

RabMAb
Recombinant
KO Validated
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(2 Publications)

Rabbit Recombinant Monoclonal CD130 (gp130) antibody. Carrier free. Suitable for WB, I-ELISA and reacts with Human, Recombinant full length protein - Human samples. Cited in 2 publications.

View Alternative Names

CD130, Interleukin-6 receptor subunit beta, IL-6 receptor subunit beta, IL-6R subunit beta, IL-6R-beta, IL-6RB, CDw130, Interleukin-6 signal transducer, Membrane glycoprotein 130, Oncostatin-M receptor subunit alpha, gp130, IL6ST

5 Images

Lab

Western blot - Anti-CD130 (gp130) antibody [EPR21732] - BSA and Azide free (AB234105)

This data was developed using the same antibody clone in a different buffer formulation (ab217671).

Lanes 1-2 : Merged signal (red and green). Green - ab217671 observed at 130 kDa. Red - loading control ab7291 observed at 50 kDa.

ab217671 Anti-CD130 (gp130) antibody [EPR21732] was shown to specifically react with CD130 (gp130) in wild-type A549 cells. Loss of signal was observed when knockout cell line ab266938 (knockout cell lysate ab257207) was used. Wild-type and CD130 (gp130) knockout samples were subjected to SDS-PAGE. ab217671 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-CD130 (gp130) antibody [EPR21732] (<a href='/en-us/products/primary-antibodies/cd130-gp130-antibody-epr21732-ab217671'>ab217671</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

IL6ST knockout A549 cell lysate at 20 µg

Lane 2:

Western blot - Human IL6ST (CD130/gp130) knockout A549 cell line (<a href='/en-us/products/cell-lines/human-il6st-cd130-gp130-knockout-a549-cell-line-ab266938'>ab266938</a>)

Predicted band size: 104 kDa

Observed band size: 130 kDa

false

Lab

Western blot - Anti-CD130 (gp130) antibody [EPR21732] - BSA and Azide free (AB234105)

This data was developed using the same antibody clone in a different buffer formulation (ab217671).

Lanes 1- 2 : Merged signal (red and green). Green - ab217671 observed at 130 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

ab217671 was shown to react with CD130 (gp130) in wild-type A549 cells in western blot. Loss of signal was observed when knockout cell line ab266939 (knockout cell lysate ab257208) was used. Wild-type A549 and IL6ST knockout A549 cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab217671 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-CD130 (gp130) antibody [EPR21732] (<a href='/en-us/products/primary-antibodies/cd130-gp130-antibody-epr21732-ab217671'>ab217671</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

IL6ST knockout A549 cell lysate at 20 µg

Lane 3:

PC3 cell lysate at 20 µg

Predicted band size: 104 kDa

Observed band size: 130 kDa

false

Lab

Western blot - Anti-CD130 (gp130) antibody [EPR21732] - BSA and Azide free (AB234105)

This data was developed using the same antibody clone in a different buffer formulation (ab217671).

Lanes 1 - 2 : Merged signal (red and green). Green - ab217671 observed at 130 kDa. Red - loading control ab8245 observed at 37 kDa.

ab217671 Anti-CD130 (gp130) antibody [EPR21732] was shown to specifically react with CD130 (gp130) in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265767 (knockout cell lysate ab257205) was used. Wild-type and CD130 (gp130) knockout samples were subjected to SDS-PAGE. ab217671 and Anti-GAPDH antibody [6C5] - Loading Control were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-CD130 (gp130) antibody [EPR21732] (<a href='/en-us/products/primary-antibodies/cd130-gp130-antibody-epr21732-ab217671'>ab217671</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

IL6ST knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human IL6ST (CD130/gp130) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-il6st-cd130-gp130-knockout-hela-cell-line-ab265767'>ab265767</a>)

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution

Predicted band size: 104 kDa

Observed band size: 130 kDa

false

Supplier Data

Western blot - Anti-CD130 (gp130) antibody [EPR21732] - BSA and Azide free (AB234105)

locking and dilution buffer : 5% NFDM/TBST.

ab217671 was shown to specifically react with CD130 (gp130) in wild-type HAP1 cells as the signal was lost in CD130 (gp130) knockout cells. Wild-type and CD130 (gp130) knockout samples were subjected to SDS-PAGE. ab217671 and ab181602 (Human anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/200000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD^® ChemiDoc™ MP instrument using the ECL technique.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab217671).

All lanes:

Western blot - Anti-CD130 (gp130) antibody [EPR21732] (<a href='/en-us/products/primary-antibodies/cd130-gp130-antibody-epr21732-ab217671'>ab217671</a>) at 1/1000 dilution

Lane 1:

Wild-type HAP1 (human chronic myelogenous leukemia cell line) whole cell lysate at 20 µg

Lane 2:

CD130 (gp130) knockout HAP1 whole cell lysate at 20 µg

Lane 3:

PC-3 (human prostate adenocarcinoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 104 kDa

Observed band size: 130 kDa

true

Exposure time: 114s

Indirect ELISA - Anti-CD130 (gp130) antibody [EPR21732] - BSA and Azide free (AB234105)

I-ELISA

Unknown

Indirect ELISA - Anti-CD130 (gp130) antibody [EPR21732] - BSA and Azide free (AB234105)

This data was developed using ab217671, the same antibody clone in a different buffer formulation.ELISA analysis of IL6ST recombinant protein at 1000 ng/mL with ab217671. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.

Unconjugated

Anti-CD130 (gp130) antibody [EPR21732]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21732

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

I-ELISA, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>We recommend that the customer optimizes the western blotting conditions.</p>", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p></p>" } } }

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Product details

ab234105 is the carrier-free version of ab217671.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD130 also known as gp130 or the IL-6 signal transducer (IL6ST) is a protein weighing around 130 kDa. This receptor is encoded by the IL6ST gene and widely expressed on the surface of cells across multiple tissues. CD130 functions mechanically as a signal transducing receptor facilitating the assembly of receptor complexes necessary for the initiation of signal transduction. It plays a major role in cytokine receptor signaling particularly interacting with interleukin-6 (IL-6) family cytokines.

Biological function summary

CD130 functions as part of the high-affinity receptor complex for certain cytokines. It partners with other receptors such as CD126 to form a complex that initiates signaling pathways after ligand binding contributing to a range of immune responses. Its role is thorough across many cellular processes including differentiation proliferation and apoptosis. In addition to immune signaling CD130 is involved in several growth factor signaling pathways.

Pathways

CD130 serves a significant role in signal transduction particularly in the Janus kinase/signal transducers and activators of transcription (JAK/STAT) pathway. It works in concert with related proteins like JAK1 and STAT3 to transmit signals from extracellular cytokines to the nucleus influencing gene expression. Additionally CD130 can also be found active in the MAPK/ERK pathway which is important for cellular response to growth signals.

CD130 plays a connection especially to inflammatory conditions and certain cancers. Aberrant signaling involving CD130 has been linked to disorders like rheumatoid arthritis where it contributes to the inflammation process. Additionally it relates to multiple myeloma a cancer where abnormal CD130 interactions with proteins like IL-6 may enhance cell survival and proliferation presenting potential targets for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Signal-transducing molecule (PubMed : 2261637). The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize IL6ST for initiating signal transmission. Binding of IL6 to IL6R induces IL6ST homodimerization and formation of a high-affinity receptor complex, which activates the intracellular JAK-MAPK and JAK-STAT3 signaling pathways (PubMed : 19915009, PubMed : 2261637, PubMed : 23294003). That causes phosphorylation of IL6ST tyrosine residues which in turn activates STAT3 (PubMed : 19915009, PubMed : 23294003, PubMed : 25731159). In parallel, the IL6 signaling pathway induces the expression of two cytokine receptor signaling inhibitors, SOCS1 and SOCS3, which inhibit JAK and terminate the activity of the IL6 signaling pathway as a negative feedback loop (By similarity). Also activates the yes-associated protein 1 (YAP) and NOTCH pathways to control inflammation-induced epithelial regeneration, independently of STAT3 (By similarity). Acts as a receptor for the neuroprotective peptide humanin as part of a complex with IL27RA/WSX1 and CNTFR (PubMed : 19386761). Mediates signals which regulate immune response, hematopoiesis, pain control and bone metabolism (By similarity). Has a role in embryonic development (By similarity). Essential for survival of motor and sensory neurons and for differentiation of astrocytes (By similarity). Required for expression of TRPA1 in nociceptive neurons (By similarity). Required for the maintenance of PTH1R expression in the osteoblast lineage and for the stimulation of PTH-induced osteoblast differentiation (By similarity). Required for normal trabecular bone mass and cortical bone composition (By similarity).. Isoform 2. Binds to the soluble IL6 : sIL6R complex (hyper-IL6), thereby blocking IL6 trans-signaling. Inhibits sIL6R-dependent acute phase response (PubMed : 11121117, PubMed : 21990364, PubMed : 30279168). Also blocks IL11 cluster signaling through IL11R (PubMed : 30279168).

See full target information IL6ST

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Bioengineered 13:13300-13311 PubMed35635079

2022

Dexmedetomidine protects H9C2 rat cardiomyocytes against hypoxia/reoxygenation injury by regulating the long non-coding RNA colon cancer-associated transcript 1/microRNA-8063/Wnt/β-catenin axis.

Applications

Unspecified application

Species

Unspecified reactive species

Chundong Liu,Rui Xu

The Journal of cell biology 220: PubMed34287648

2021

The glycoprotein GP130 governs the surface presentation of the G protein-coupled receptor APLNR.

Applications

Unspecified application

Species

Unspecified reactive species

Kilian Trillet,Kathryn A Jacobs,Gwennan André-Grégoire,An Thys,Clément Maghe,Jonathan Cruard,Stéphane Minvielle,Sara Gonzalez Diest,Guillaume Montagnac,Nicolas Bidère,Julie Gavard

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD130 (gp130) antibody [EPR21732] - BSA and Azide free

Western blot - Anti-CD130 (gp130) antibody [EPR21732] - BSA and Azide free (AB234105)

Western blot - Anti-CD130 (gp130) antibody [EPR21732] - BSA and Azide free (AB234105)

Western blot - Anti-CD130 (gp130) antibody [EPR21732] - BSA and Azide free (AB234105)

Western blot - Anti-CD130 (gp130) antibody [EPR21732] - BSA and Azide free (AB234105)

Indirect ELISA - Anti-CD130 (gp130) antibody [EPR21732] - BSA and Azide free (AB234105)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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