AB276130

Anti-CD133 antibody [BLR093G]

Recombinant
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(3 Publications)

Rabbit Recombinant Monoclonal CD133 antibody. Suitable for IHC-P, ICC, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human PROM1 aa 350-400.

View Alternative Names

CD133, PROML1, MSTP061, PROM1, Prominin-1, Antigen AC133, Prominin-like protein 1

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [BLR093G] (AB276130)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [BLR093G] (AB276130)

Paraffin embedded human colon carcinoma tissue stained for CD133 using ab276130 at 1/500 dilution in immunohistochemical analysis.

Supplier Data

Immunocytochemistry - Anti-CD133 antibody [BLR093G] (AB276130)

OVCAR-4 cells stained for CD133 using ab276130 at 1/500 dilution in immunocytochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [BLR093G] (AB276130)

Paraffin embedded human colon carcinoma tissue stained for CD133 using ab276130 at 1/500 dilution in immunohistochemical analysis.

Supplier Data

Immunocytochemistry - Anti-CD133 antibody [BLR093G] (AB276130)

SW620 cells stained for CD133 using ab276130 at 1/500 dilution in immunocytochemical analysis

Supplier Data

Western blot - Anti-CD133 antibody [BLR093G] (AB276130)

Chemiluminescence with an exposure time of 3 minutes.

Lower Panel : Rabbit anti-COPB2 antibody.

All lanes:

Western blot - Anti-CD133 antibody [BLR093G] (ab276130) at 1/1000 dilution

Lane 1:

HEK-293T whole cell lysate at 50 µg

Lane 2:

SW620 whole cell lysate at 50 µg

Lane 3:

GaMG whole cell lysate at 50 µg

Lane 4:

OVCAR-4 whole cell lysate at 50 µg

Lane 5:

U-2 OS whole cell lysate at 50 µg

Lane 6:

HepG2 whole cell lysate at 50 µg

Lane 7:

A549 whole cell lysate at 50 µg

Lane 8:

MCF7 whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

Predicted band size: 97 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR093G

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC, IHC-P

applications

Immunogen

Synthetic Peptide within Human PROM1 aa 350-400. The exact immunogen used to generate this antibody is proprietary information.

O43490

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/100 - 1/500", "ICC-species-notes": "Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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Product details

This product is sold under License from Bethyl Laboratories, Inc.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 8.2 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD133 also known as Prominin-1 is a transmembrane glycoprotein with a molecular weight of approximately 120 kDa. This protein stands out due to its expression in various stem and progenitor cells including those of the neural hematopoietic and epithelial lineages. CD133 is recognized for its distinct five transmembrane domains and two large extracellular loops. Researchers commonly use CD133 as a cell surface marker to identify stem and progenitor cells. The significance of CD133 extends to immunohistochemistry (IHC) and Western blot applications where it is a popular target for detection and analysis.

Biological function summary

CD133 is involved in maintaining the cellular architecture and signaling processes. It plays a role in cellular differentiation and proliferation while acting as a part of larger protein complexes on the cell surface. This protein is notable as it contributes significantly to the regulation of stem cell properties supporting the growth and repair in various tissues. Anti-CD133 antibodies are valuable tools in studying these functions due to their specificity in targeting this marker.

Pathways

CD133 participates in cellular signaling and transport pathways vital for intercellular communication and homeostasis. It interacts within pathways related to cell polarity and asymmetric division both essential for tissue development and maintenance. CD133 is associated with proteins like CD133/1 and CD133/2 which further highlights its involvement in these complex biological networks and on pathways such as the Wnt signaling pathway which regulates gene expression associated with cell proliferation and differentiation.

Several studies indicate a strong relationship between CD133 expression and certain cancers such as glioblastoma and colorectal cancer. In these cases CD133 serves not only as a marker but also as an indicator of the presence of cancer stem cells which are capable of tumor initiation and maintenance. Furthermore researchers have identified connections between CD133 and other proteins such as c-Met (cMab) within these pathological contexts suggesting interactions that could contribute to disease progression and offering potential targets for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May play a role in cell differentiation, proliferation and apoptosis (PubMed : 24556617). Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner (PubMed : 20818439).

See full target information PROM1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Biological research 58:10 PubMed39980072

2025

Calcium electroporation induces stress response through upregulation of HSP27, HSP70, aspartate β-hydroxylase, and CD133 in human colon cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Szewczyk,Nina Rembiałkowska,Jolanta Saczko,Małgorzata Daczewska,Vitalij Novickij,Julita Kulbacka

Scientific reports 14:16498 PubMed39020017

2024

The role of lipid peroxidation in epithelial-mesenchymal transition of retinal pigment epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Wang You,Kunihiro Azuma,Toshiro Iwagawa,Sumiko Watanabe,Makoto Aihara,Tomoyasu Shiraya,Takashi Ueta

Frontiers in cell and developmental biology 9:659731 PubMed34631691

2021

FOXO4 Inhibits the Migration and Metastasis of Colorectal Cancer by Regulating the /β-Catenin Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Sun,Lin Wang,Xuehu Xu,Puqing Han,Jinghao Wu,Xuan Tian,Mingsong Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD133 antibody [BLR093G]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [BLR093G] (AB276130)

Immunocytochemistry - Anti-CD133 antibody [BLR093G] (AB276130)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [BLR093G] (AB276130)

Immunocytochemistry - Anti-CD133 antibody [BLR093G] (AB276130)

Western blot - Anti-CD133 antibody [BLR093G] (AB276130)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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Primary Antibodies

Primary Antibodies

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