Anti-CD133 antibody [CMab-43] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [CMab-43] - BSA and Azide free (AB264545)

Immunohistochemical analysis of paraffin-embedded human endometrium carcinoma tissue labeling CD133 with ab264538 at 1/2000 dilution followed by a ready to use secondary. Apical staining on ducts of human endometrium carcinoma. The section was incubated with ab264538 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use.

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab264538).

• IHC-P

AbReview78194****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [CMab-43] - BSA and Azide free (AB264545)

Immunohistochemistry analysis (Formalin/PFA-fixed paraffin-embedded sections) of formaldehyde fixed human cSCC tumor tissue. Stained with ab264545 at 1/1000 dilution. Secondary antibody used was HRP Peroxidase AffiniPure Goat Anti-Mouse IgG (H+L) at 1/50 dilution. Blocking was done with 3% serum for 30 minutes at room temperature. The sample was incubated with the primary antibody and Blocking buffer for 1 hour 30 minutes. Antigen retrieval method was heat mediated

This image is courtesy of an anonymous Abreview

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [CMab-43] - BSA and Azide free (AB264545)

Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling CD133 with ab264538 at 1/2000 dilution followed by a ready to use secondary. Apical staining on ducts of human pancreas. The section was incubated with ab264538 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use.

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab264538).

• WB

Lab

Western blot - Anti-CD133 antibody [CMab-43] - BSA and Azide free (AB264545)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The 128KDa band is glycosylated form which is consistent with what has been described in the literature (PMID : 28910211, 20199106, 30873590).

Exposure time : 7 seconds.

This image was produced using the same antibody clone but in a different formulation ab264538, PBS, sodium azide, glycerol and BSA.

All lanes:

Western blot - Anti-CD133 antibody [CMab-43] (<a href='/en-us/products/primary-antibodies/cd133-antibody-cmab-43-ab264538'>ab264538</a>) at 1/1000 dilution

Lane 1:

Caco-2 (human colorectal adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

HT-29 (human colorectal adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 3:

NCCIT (human pluripotent embryonic carcinoma epithelial cell), whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 97 kDa

Observed band size: 128 kDa

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