Anti-CD133 antibody [EPR16508]

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [EPR16508] (AB222782)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling CD133 with ab222782 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ready to use). Membranous staining on parietal layer of Bowman's capsule and apical staining on renal tubules in human kidney (PMID : 19092120). Counter stained with Hematoxylin. Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ready to use).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [EPR16508] (AB222782)

Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling CD133 with ab222782 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP)(ready use). Apical/endoluminal staining of human pancreatic ducts, and negative on Langerhans cells of the islets (PMID : 18261235). Counter stained with Hematoxylin. Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP)(ready to use).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [EPR16508] (AB222782)

Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling CD133 with ab222782 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ready to use). Membranous staining on human bladder cancer tumor cells (PMID : 22899341). Counter stained with Hematoxylin. Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ready to use).

• WB

Lab

Western blot - Anti-CD133 antibody [EPR16508] (AB222782)

ab222782 was shown to react with CD133 in wild-type HAP1 cells in western blot. Loss of signal was observed when PROM1 knockout sample was used. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween^®) before incubation with ab222782 overnight at 4°C at a 1 in 2000 dilution. Blots were incubated with HRP conjugated Goat anti-Rabbit (H+L) secondary antibody at 1/5000 for 1 hour at room temperature before development with ECL reagent and imaging.

All lanes:

Western blot - Anti-CD133 antibody [EPR16508] (ab222782) at 1/2000 dilution

Lane 1:

Wild-type HAP1 cell lysate at 40 µg

Lane 2:

PROM1 knockout HAP1 cell lysate at 40 µg

Predicted band size: 97 kDa

Observed band size: 110 kDa

false

Exposure time: 8min

• WB

Lab

Western blot - Anti-CD133 antibody [EPR16508] (AB222782)

Blocking buffer : 5% NFDM/TBST.

The MW observed is consistent with the literature (PMID 20717901; PMID 18645205).

All lanes:

Western blot - Anti-CD133 antibody [EPR16508] (ab222782) at 1/2000 dilution

Lane 1:

Human fetal pancreas lysate at 20 µg

Lane 2:

Human fetal kidney lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 97 kDa

false

Exposure time: 3min

• WB

Lab

Western blot - Anti-CD133 antibody [EPR16508] (AB222782)

Blocking buffer : 5% NFDM/TBST.

The MW observed is consistent with the literature (PMID 20717901; PMID 18645205).

All lanes:

Western blot - Anti-CD133 antibody [EPR16508] (ab222782) at 1/10000 dilution

All lanes:

NCCIT (Human pluripotent embryonic carcinoma epithelial cell), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 97 kDa

false

Exposure time: 15s

• WB

Lab

Western blot - Anti-CD133 antibody [EPR16508] (AB222782)

Negative control : HeLa (PMID 9389721).

The MW observed is consistent with the literature (PMID 20717901; PMID 18645205).

Blocking buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CD133 antibody [EPR16508] (ab222782) at 1/10000 dilution

Lane 1:

Caco-2 (Human colorectal adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

HT-29 (Human colorectal adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 3:

HeLa (Human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 97 kDa

false

Exposure time: 5s

• WB

CiteAb

Western blot - Anti-CD133 antibody [EPR16508] (AB222782)

CD133 western blot using anti-CD133 antibody [EPR16508] ab222782. Publication image and figure legend from Shi, M., An, G., et al., 2023, Cancers (Basel), PubMed 37173968.

ab222782 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab222782 please see the product overview.

Correlation between UVRAG expression and therapeutic resistance. (A) Relative expression of UVRAG in HCT8, HCT8-5Fu, and HCT8-60Gy cells. (B) The sensitivity of HCT8-CON and HCT8-UVRAG cells to radiotherapy. (C) UVRAG knockdown increased 5-Fu sensitivity. (D) UVRAG overexpression decreased 5-Fu sensitivity. (E) Relative mRNA expression of UVRAG in HCT116 and HCT116-self cells. (F) Relative mRNA expression of UVRAG in HCT116 DCLK- and HCT116 DCLK+ cells. (G) Western blot of PD-L1, UVARG, ABCG2, BMI1, and SP1 after upregulating UVARG or downregulating UVRAG. The uncropped blots are shown in Figure S1. (H) Spheroid formation in UVRAG overexpressed HCT116 and its control cells. (I) Stem cell score evaluated via ssGSEA in patients with UVRAG higher expression and UVARG lower expression. Independent-samples t-test (A,E,F). Mann Whitney U test (I). * p < 0.05, ** p < 0.01.

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