Rabbit Monoclonal CD133 antibody. Carrier free. Suitable for IP, Flow Cyt, WB, IHC-P and reacts with Human samples.
Images
![Flow Cytometry - Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (AB226484), expandable thumbnail](https://content.abcam.com/products/images/cd133-antibody-epr20980-104-bsa-and-azide-free-ab226484--flow-cytometry-img71387.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (AB226484)")
![Western blot - Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (AB226484), expandable thumbnail](https://content.abcam.com/products/images/cd133-antibody-epr20980-104-bsa-and-azide-free-ab226484--western-blot-img113127.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (AB226484)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (AB226484), expandable thumbnail](https://content.abcam.com/products/images/cd133-antibody-epr20980-104-bsa-and-azide-free-ab226484--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130399.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (AB226484)")
![Immunoprecipitation - Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (AB226484), expandable thumbnail](https://content.abcam.com/products/images/cd133-antibody-epr20980-104-bsa-and-azide-free-ab226484--immunoprecipitation-img138.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (AB226484)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (AB226484), expandable thumbnail](https://content.abcam.com/products/images/cd133-antibody-epr20980-104-bsa-and-azide-free-ab226484--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img95040.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (AB226484)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
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5 products for Alternative Version
Target data
Function
May play a role in cell differentiation, proliferation and apoptosis (PubMed:24556617). Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner (PubMed:20818439).
Alternative names
Prominin-1, Antigen AC133, Prominin-like protein 1, MSTP061, PROML1, PROM1
Recommended products
Rabbit Monoclonal CD133 antibody. Carrier free. Suitable for IP, Flow Cyt, WB, IHC-P and reacts with Human samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR20980-104
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab226484 is the carrier-free version of Anti-CD133 antibody [EPR20980-104] ab216323.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD133 also known as Prominin-1 is a transmembrane glycoprotein with a molecular weight of approximately 120 kDa. This protein stands out due to its expression in various stem and progenitor cells including those of the neural hematopoietic and epithelial lineages. CD133 is recognized for its distinct five transmembrane domains and two large extracellular loops. Researchers commonly use CD133 as a cell surface marker to identify stem and progenitor cells. The significance of CD133 extends to immunohistochemistry (IHC) and Western blot applications where it is a popular target for detection and analysis.
- Biological function summary
CD133 is involved in maintaining the cellular architecture and signaling processes. It plays a role in cellular differentiation and proliferation while acting as a part of larger protein complexes on the cell surface. This protein is notable as it contributes significantly to the regulation of stem cell properties supporting the growth and repair in various tissues. Anti-CD133 antibodies are valuable tools in studying these functions due to their specificity in targeting this marker.
- Pathways
CD133 participates in cellular signaling and transport pathways vital for intercellular communication and homeostasis. It interacts within pathways related to cell polarity and asymmetric division both essential for tissue development and maintenance. CD133 is associated with proteins like CD133/1 and CD133/2 which further highlights its involvement in these complex biological networks and on pathways such as the Wnt signaling pathway which regulates gene expression associated with cell proliferation and differentiation.
- Associated diseases and disorders
Several studies indicate a strong relationship between CD133 expression and certain cancers such as glioblastoma and colorectal cancer. In these cases CD133 serves not only as a marker but also as an indicator of the presence of cancer stem cells which are capable of tumor initiation and maintenance. Furthermore researchers have identified connections between CD133 and other proteins such as c-Met (cMab) within these pathological contexts suggesting interactions that could contribute to disease progression and offering potential targets for therapeutic intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Flow Cytometry - Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (ab226484), expandable thumbnail](https://content.abcam.com/products/images/cd133-antibody-epr20980-104-bsa-and-azide-free-ab226484--flow-cytometry-img71387.jpg "Flow Cytometry - Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (ab226484)")
Flow Cytometry - Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (ab226484)
Flow cytometric analysis of HEK-293T (human embryonic kidney epithelial cell)(Left) / Caco-2 (human colorectal adenocarcinoma epithelial cell)(Right) cell line labeling CD133 with Anti-CD133 antibody [EPR20980-104] ab216323 at 1/100 (Red) compared with Isotype Control Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (BLack) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody.
Negative control: 293T (PMID 20167130). Total viable cells were gated for the FC image.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD133 antibody [EPR20980-104] ab216323).
![Western blot - Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (ab226484), expandable thumbnail](https://content.abcam.com/products/images/cd133-antibody-epr20980-104-bsa-and-azide-free-ab226484--western-blot-img113127.jpg "Western blot - Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (ab226484)")
Western blot - Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (ab226484)
Anti-CD133 antibody [EPR20980-104] ab216323 was shown to react with CD133 in western blot. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with Anti-CD133 antibody [EPR20980-104] ab216323 overnight at 4°C at a 1 in 1000 dilution. Blots were incubated with HRP conjugated Goat anti-Rabbit (H+L) secondary antibody at 1/5000 for 1 hour at room temperature before development with ECL reagent and imaging.
All lanes: Western blot - Anti-CD133 antibody [EPR20980-104] (Anti-CD133 antibody [EPR20980-104] ab216323) at 1/1000 dilution
Lane 1: Caco-2 cell lysate at 20 µg
Lane 2: HeLa cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 97 kDa
Observed band size: 110 kDa
Exposure time: 8min
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (ab226484), expandable thumbnail](https://content.abcam.com/products/images/cd133-antibody-epr20980-104-bsa-and-azide-free-ab226484--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130399.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (ab226484)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (ab226484)
Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling CD133 with Anti-CD133 antibody [EPR20980-104] ab216323 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP), ready to use. Apical/endoluminal staining on ducts of human pancreas, and negative on Langerhans cells of the islets (PMID: 18261235) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP), ready to use.
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD133 antibody [EPR20980-104] ab216323).
![Immunoprecipitation - Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (ab226484), expandable thumbnail](https://content.abcam.com/products/images/cd133-antibody-epr20980-104-bsa-and-azide-free-ab226484--immunoprecipitation-img138.jpg "Immunoprecipitation - Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (ab226484)")
Immunoprecipitation - Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (ab226484)
CD133 was immunoprecipitated from 0.35 mg Caco-2 (human colorectal adenocarcinoma epithelial cell) whole cell lysate with Anti-CD133 antibody [EPR20980-104] ab216323 at 1/70 dilution. Western blot was performed from the immunoprecipitate using Anti-CD133 antibody [EPR20980-104] ab216323 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1000 dilution.
Lane 1: Caco-2 (human colorectal adenocarcinoma epithelial cell) whole cell lysate 10 μg (Input).
Lane 2: Anti-CD133 antibody [EPR20980-104] ab216323 IP in Caco-2 whole cell lysate (+).
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CD133 antibody [EPR20980-104] ab216323 in Caco-2 whole cell lysate (-).
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD133 antibody [EPR20980-104] ab216323).
All lanes: Immunoprecipitation - Anti-CD133 antibody [EPR20980-104] (Anti-CD133 antibody [EPR20980-104] ab216323)
Predicted band size: 97 kDa
Observed band size: 120 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (ab226484), expandable thumbnail](https://content.abcam.com/products/images/cd133-antibody-epr20980-104-bsa-and-azide-free-ab226484--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img95040.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (ab226484)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (ab226484)
Immunohistochemical analysis of paraffin-embedded human mammary gland tissue labeling CD133 with Anti-CD133 antibody [EPR20980-104] ab216323 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP), ready to use. Apical/endoluminal staining on ducts of human mammary gland (PMID: 18261235) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP), ready to use.
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD133 antibody [EPR20980-104] ab216323).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (ab226484), expandable thumbnail](https://content.abcam.com/products/images/cd133-antibody-epr20980-104-bsa-and-azide-free-ab226484--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img38530.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (ab226484)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (ab226484)
Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling CD133 with Anti-CD133 antibody [EPR20980-104] ab216323 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP), ready to use. Membranous staining on parietal layer of Bowman’s capsule of human kidney (PMID: 19092120) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP), ready to use.
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD133 antibody [EPR20980-104] ab216323).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (ab226484), expandable thumbnail](https://content.abcam.com/products/images/cd133-antibody-epr20980-104-bsa-and-azide-free-ab226484--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img418521.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (ab226484)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (ab226484)
Immunohistochemical analysis of paraffin-embedded Human pancreatic carcinoma tissue labelling CD133 with Anti-CD133 antibody [EPR20980-104] ab216323 at 1/1000 dilution (1.386 μg/ml) followed by a Goat Anti-Rabbit IgG H&L (HRP polymer) (Goat Anti-Rabbit IgG H&L (HRP polymer) ab214880) at Ready to use dilution. Positive staining on human pancreatic carcinoma. The section was incubated with Anti-CD133 antibody [EPR20980-104] ab216323 at 4°C overnight. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (HRP polymer) (Goat Anti-Rabbit IgG H&L (HRP polymer) ab214880) at Ready to use dilution.
Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD133 antibody [EPR20980-104] ab216323).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (ab226484), expandable thumbnail](https://content.abcam.com/products/images/cd133-antibody-epr20980-104-bsa-and-azide-free-ab226484--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img38530.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (ab226484)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (ab226484), expandable thumbnail](https://content.abcam.com/products/images/cd133-antibody-epr20980-104-bsa-and-azide-free-ab226484--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img418521.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [EPR20980-104] - BSA and Azide free (ab226484)")
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