Anti-CD133 antibody [EPR24348-38] (ab271092)

Rabbit Recombinant Monoclonal CD133 antibody. Suitable for IP, Flow Cyt (Intra), WB, IHC-P, IHC-Fr and reacts with Mouse samples. Cited in 2 publications.

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Images

Flow Cytometry (Intracellular) - Anti-CD133 antibody [EPR24348-38] (AB271092), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	ICC/IF	IP	Flow Cyt (Intra)	WB	IHC-P	IHC-Fr
Human	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended
Mouse	Not recommended	Tested	Tested	Tested	Tested	Tested
Rat	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat, Human, Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/30	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/50	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/2000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/100	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat, Human	Dilution info -	Notes -

Target data

See full target information Prom1

Function

May play a role in cell differentiation, proliferation and apoptosis. Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis (PubMed:19228982). Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner.

Alternative names

CD133, Prom, Proml1, Prom1, Prominin-1, Antigen AC133 homolog, Prominin-like protein 1

Recommended products

Rabbit Recombinant Monoclonal CD133 antibody. Suitable for IP, Flow Cyt (Intra), WB, IHC-P, IHC-Fr and reacts with Mouse samples. Cited in 2 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR24348-38
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD133 also known as Prominin-1 is a transmembrane glycoprotein with a molecular weight of approximately 120 kDa. This protein stands out due to its expression in various stem and progenitor cells including those of the neural hematopoietic and epithelial lineages. CD133 is recognized for its distinct five transmembrane domains and two large extracellular loops. Researchers commonly use CD133 as a cell surface marker to identify stem and progenitor cells. The significance of CD133 extends to immunohistochemistry (IHC) and Western blot applications where it is a popular target for detection and analysis.

Biological function summary

CD133 is involved in maintaining the cellular architecture and signaling processes. It plays a role in cellular differentiation and proliferation while acting as a part of larger protein complexes on the cell surface. This protein is notable as it contributes significantly to the regulation of stem cell properties supporting the growth and repair in various tissues. Anti-CD133 antibodies are valuable tools in studying these functions due to their specificity in targeting this marker.

Pathways

CD133 participates in cellular signaling and transport pathways vital for intercellular communication and homeostasis. It interacts within pathways related to cell polarity and asymmetric division both essential for tissue development and maintenance. CD133 is associated with proteins like CD133/1 and CD133/2 which further highlights its involvement in these complex biological networks and on pathways such as the Wnt signaling pathway which regulates gene expression associated with cell proliferation and differentiation.

Associated diseases and disorders

Several studies indicate a strong relationship between CD133 expression and certain cancers such as glioblastoma and colorectal cancer. In these cases CD133 serves not only as a marker but also as an indicator of the presence of cancer stem cells which are capable of tumor initiation and maintenance. Furthermore researchers have identified connections between CD133 and other proteins such as c-Met (cMab) within these pathological contexts suggesting interactions that could contribute to disease progression and offering potential targets for therapeutic intervention.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

13 product images

Flow Cytometry (Intracellular) - Anti-CD133 antibody [EPR24348-38] (ab271092)
Flow Cytometry analysis of Mouse bone marrow cells labelling CD133 with ab271092 at 1/50 (right). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 was used as the secondary antibody. Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) was used as the isotype control (left). Gated on viable cells.
Flow Cytometry (Intracellular) - Anti-CD133 antibody [EPR24348-38] (ab271092)
Flow Cytometry analysis of Mouse bone marrow cells labelling CD133 with ab271092 at 1/50 (right). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat Anti-Rabbit IgG (Alexa Fluor® 647) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed ab150083) at 1/2000 was used as the secondary antibody. Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) was used as the isotype control (left). Cells were stained with rabbit IgG or ab271092. Then stained with Lineage cocktail conjugated to FITC, anti-Sca-1 conjugated to PE/Cy7, anti-CD117 conjugated to APC/Cy7 and anti-CD16/32 conjugated to BV510.
Gated on viable MP cells. Positive staining on CD16/32+ MP cells is consistent with literature. (PMID: 23509298).
Western blot - Anti-CD133 antibody [EPR24348-38] (ab271092)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
All lanes: Western blot - Anti-CD133 antibody [EPR24348-38] (ab271092) at 1/1000 dilution
Lane 1: Mouse kidney tissue lysate
Lane 2: Mouse kidney tissue lysate treated with PNGase F
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 97 kDa
Observed band size: 120 kDa, 97 kDa
Exposure time: 10s
Flow Cytometry (Intracellular) - Anti-CD133 antibody [EPR24348-38] (ab271092)
Flow Cytometry analysis of Mouse bone marrow cells labelling CD133 with ab271092 at 1/50 (right). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 was used as the secondary antibody. Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) was used as the isotype control (left). Cells were co-stained with anti-CD117 conjugated to BV421. Gated on viable cells.
Western blot - Anti-CD133 antibody [EPR24348-38] (ab271092)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: mouse heart (PMID:17575139).
Exposure time: Lanes 1-2: 10 seconds; Lane 3: 70 seconds.
All lanes: Western blot - Anti-CD133 antibody [EPR24348-38] (ab271092) at 1/1000 dilution
Lane 1: Mouse kidney tissue lysate at 20 µg
Lane 2: Mouse heart tissue lysate at 20 µg
Lane 3: Mouse E14.5 brain tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 97 kDa
Observed band size: 120 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [EPR24348-38] (ab271092)
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labelling CD133 with ab271092 at 1/2000 (0.23 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the biliary ducts of mouse liver. The section was incubated with ab271092 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [EPR24348-38] (ab271092)
Immunohistochemical analysis of paraffin-embedded Mouse pancreas tissue labelling CD133 with ab271092 at 1/2000 (0.23 ug/ml) followed by a ready to use LeicaDS9800(Bond™ Polymer Refine Detection). Positive staining on mouse pancreatic ducts. The section was incubated with ab271092 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [EPR24348-38] (ab271092)
Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labelling CD133 with ab271092 at 1/2000 (0.23 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse kidney. The section was incubated with ab271092 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [EPR24348-38] (ab271092)
Immunohistochemical analysis of paraffin-embedded Mouse retina tissue labelling CD133 with ab271092 at 1/2000 (0.23 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the cone and rod cells of mouse retina. The section was incubated with ab271092 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunoprecipitation - Anti-CD133 antibody [EPR24348-38] (ab271092)
CD133 was immunoprecipitated from 0.35 mg Mouse E14.5 brain tissue lysate 10 ug with ab271092 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab271092 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Mouse E14.5 brain tissue lysate 10 ug
Lane 2: ab271092 IP in Mouse E14.5 brain tissue lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab271092 in Mouse E14.5 brain tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds
All lanes: Immunoprecipitation - Anti-CD133 antibody [EPR24348-38] (ab271092)
Predicted band size: 97 kDa
Observed band size: 120 kDa
Immunohistochemistry (Frozen sections) - Anti-CD133 antibody [EPR24348-38] (ab271092)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse E14.5 embryonic kidney tissue labeling CD133 with ab271092 at 1/100 (4.59 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on the apical membrane of mouse E14.5 embryonic kidney is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunohistochemistry (Frozen sections) - Anti-CD133 antibody [EPR24348-38] (ab271092)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse E14.5 embryonic cortex tissue labeling CD133 with ab271092 at 1/100 (4.59 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on the apical membrane of mouse E14.5 embryonic cortex is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunohistochemistry (Frozen sections) - Anti-CD133 antibody [EPR24348-38] (ab271092)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse E14.5 embryonic lung tissue labeling CD133 with ab271092 at 1/100 (4.59 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on the apical membrane of lung buds is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).