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Rabbit Recombinant Multiclonal CD133 antibody. Stem Cell marker. Suitable for IP, Flow Cyt, WB, IHC-P and reacts with Human samples. Cited in 6 publications.

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Images

Flow Cytometry - Anti-CD133 antibody [RM1002] - Stem Cell Marker (AB278053), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-CD133 antibody [RM1002] - Stem Cell Marker (AB278053), expandable thumbnail
  • Flow Cytometry - Anti-CD133 antibody [RM1002] - Stem Cell Marker (AB278053), expandable thumbnail
  • Western blot - Anti-CD133 antibody [RM1002] - Stem Cell Marker (AB278053), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [RM1002] - Stem Cell Marker (AB278053), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Multiclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPFlow CytWBIHC-FrIHC-P
Human
Not recommended
Tested
Tested
Tested
Not recommended
Tested
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
-
Notes

-

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

May play a role in cell differentiation, proliferation and apoptosis (PubMed:24556617). Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner (PubMed:20818439).

Alternative names

CD133, PROML1, MSTP061, PROM1, Prominin-1, Antigen AC133, Prominin-like protein 1

Recommended products

Rabbit Recombinant Multiclonal CD133 antibody. Stem Cell marker. Suitable for IP, Flow Cyt, WB, IHC-P and reacts with Human samples. Cited in 6 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Multiclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
RM1002
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant multiclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CD133 also known as Prominin-1 is a transmembrane glycoprotein with a molecular weight of approximately 120 kDa. This protein stands out due to its expression in various stem and progenitor cells including those of the neural hematopoietic and epithelial lineages. CD133 is recognized for its distinct five transmembrane domains and two large extracellular loops. Researchers commonly use CD133 as a cell surface marker to identify stem and progenitor cells. The significance of CD133 extends to immunohistochemistry (IHC) and Western blot applications where it is a popular target for detection and analysis.

Biological function summary

CD133 is involved in maintaining the cellular architecture and signaling processes. It plays a role in cellular differentiation and proliferation while acting as a part of larger protein complexes on the cell surface. This protein is notable as it contributes significantly to the regulation of stem cell properties supporting the growth and repair in various tissues. Anti-CD133 antibodies are valuable tools in studying these functions due to their specificity in targeting this marker.

Pathways

CD133 participates in cellular signaling and transport pathways vital for intercellular communication and homeostasis. It interacts within pathways related to cell polarity and asymmetric division both essential for tissue development and maintenance. CD133 is associated with proteins like CD133/1 and CD133/2 which further highlights its involvement in these complex biological networks and on pathways such as the Wnt signaling pathway which regulates gene expression associated with cell proliferation and differentiation.

Associated diseases and disorders

Several studies indicate a strong relationship between CD133 expression and certain cancers such as glioblastoma and colorectal cancer. In these cases CD133 serves not only as a marker but also as an indicator of the presence of cancer stem cells which are capable of tumor initiation and maintenance. Furthermore researchers have identified connections between CD133 and other proteins such as c-Met (cMab) within these pathological contexts suggesting interactions that could contribute to disease progression and offering potential targets for therapeutic intervention.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Flow Cytometry - Anti-CD133 antibody [RM1002] - Stem Cell Marker (ab278053), expandable thumbnail

    Flow Cytometry - Anti-CD133 antibody [RM1002] - Stem Cell Marker (ab278053)

    Flow cytometric analysis of HEK-293T (Human embryonic kidney epithelial cell, Left panel) / Caco-2 (Human colorectal adenocarcinoma epithelial cell, Right panel) using ab278053 at 1/500 dilution (0.1µg) (red). The isotype control used was the Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730), black line and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Secondary antibody used was the Goat anti-rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution.

    Negative control: HEK-293T. (PMID 20167130)
    Gated on viable cells.

  • Immunohistochemistry (Frozen sections) - Anti-CD133 antibody [RM1002] - Stem Cell Marker (ab278053), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-CD133 antibody [RM1002] - Stem Cell Marker (ab278053)

    IHC image of CD133 staining in a section of frozen normal human tonsil* performed on a Leica BOND™ system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab278053, 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Flow Cytometry - Anti-CD133 antibody [RM1002] - Stem Cell Marker (ab278053), expandable thumbnail

    Flow Cytometry - Anti-CD133 antibody [RM1002] - Stem Cell Marker (ab278053)

    Flow cytometric analysis of HEK-293T (Human embryonic kidney epithelial cell, Left panel) / Caco-2 (Human colorectal adenocarcinoma epithelial cell, Right panel ) using ab278053 at 1/500 dilution (0.1μg) (Red). Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti mouse IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

    Negative control: HEK-293T. (PMID 20167130)
    Gated on viable cells.

  • Western blot - Anti-CD133 antibody [RM1002] - Stem Cell Marker (ab278053), expandable thumbnail

    Western blot - Anti-CD133 antibody [RM1002] - Stem Cell Marker (ab278053)

    ab278053 was shown to react with PROM1 in wild-type HAP1 cells in western blot. Loss of signal was observed when PROM1 knockout sample was used. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab278053 overnight at 4°C at a 1 in 1000 dilution. Blots were incubated with HRP conjugated Goat anti-Rabbit (H+L) secondary antibody at 1/5000 for 1 hour at room temperature before development with Optiblot ECL reagent (Anti-PKC delta (phospho S299) antibody [EPNCI119] ab133456) and imaging.

    All lanes: Western blot - Anti-CD133 antibody [RM1002] - Stem Cell Marker (ab278053) at 1/1000 dilution

    Lane 1: Wild-type HAP1 cell lysate at 40 µg

    Lane 2: PROM1 knockout HAP1 cell lysate at 40 µg

    Secondary

    All lanes: HRP conjugated Goat anti-Rabbit (H+L) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size: 97 kDa

    Observed band size: 120 kDa

    Exposure time: 20s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [RM1002] - Stem Cell Marker (ab278053), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [RM1002] - Stem Cell Marker (ab278053)

    Immunohistochemical analysis of paraffin-embedded human mammary gland tissue labeling CD133 with ab278053 at 1/1000 (0.5 μg/ml) dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Apical staining on human mammary gland (PMID: 18261235). The section was incubated with ab278053 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [RM1002] - Stem Cell Marker (ab278053), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [RM1002] - Stem Cell Marker (ab278053)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling CD133 with ab278053 at 1/1000 (0.5 μg/ml) dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on Bowman's capsule of human kidney (PMID: 19875807). The section was incubated with ab278053 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunoprecipitation - Anti-CD133 antibody [RM1002] - Stem Cell Marker (ab278053), expandable thumbnail

    Immunoprecipitation - Anti-CD133 antibody [RM1002] - Stem Cell Marker (ab278053)

    CD133 was immunoprecipitated from 0.35 mg Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate with ab278053 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab278053 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Caco-2 whole cell lysate 10 μg.

    Lane 2: ab278053 IP in Caco-2 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab278053 in Caco-2 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

    All lanes: Immunoprecipitation - Anti-CD133 antibody [RM1002] - Stem Cell Marker (ab278053)

    Predicted band size: 97 kDa

    Observed band size: 120 kDa

  • Western blot - Anti-CD133 antibody [RM1002] - Stem Cell Marker (ab278053), expandable thumbnail

    Western blot - Anti-CD133 antibody [RM1002] - Stem Cell Marker (ab278053)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Negative control: HEK-293T (PMID 20167130).

    The MW observed is consistent with the literature (PMID 20717901; PMID 18645205).

    All lanes: Western blot - Anti-CD133 antibody [RM1002] - Stem Cell Marker (ab278053) at 1/1000 dilution

    Lane 1: Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: HEK-293T (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 3: NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 4: HT-29 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 97 kDa

    Observed band size: 120 kDa

    Exposure time: 26s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [RM1002] - Stem Cell Marker (ab278053), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [RM1002] - Stem Cell Marker (ab278053)

    Immunohistochemical analysis of paraffin-embedded human pancreatic carcinoma tissue labelling CD133 with ab278053 at 1:1000 dilution (0.5 μg/ml), followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human pancreatic carcinoma is observed. The section was incubated with ab278053 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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