Anti-CD133 antibody - Stem Cell Marker is a rabbit polyclonal antibody that is used to detect CD133 in Flow cytometry, ICC, ICC/IF, IHC-Fr, IHC-P, IP, Western blot. Suitable for Human, Mouse, Rat samples.
- Stem Cell Marker
- Cited in over 260 publications
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Publications
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- International journal of molecular sciences 24:2023Can CD133 Be Regarded as a Prognostic Biomarker in Oncology: Pros and Cons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlisa Gisina,Yan Kim,Konstantin Yarygin,Alexey LupatovPubMed 38139228
- Nature communications 14:78632023SPINK1-induced tumor plasticity provides a therapeutic window for chemotherapy in hepatocellular carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKi-Fong Man,Lei Zhou,Huajian Yu,Ka-Hei Lam,Wei Cheng,Jun Yu,Terence K Lee,Jing-Ping Yun,Xin-Yuan Guan,Ming Liu,Stephanie MaPubMed 38030644
- Frontiers in cell and developmental biology 11:12228092023The role of hypoxia and radiation in developing a CTCs-like phenotype in murine osteosarcoma cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMartina Quartieri,Anggraeini Puspitasari,Tamara Vitacchio,Marco Durante,Walter TinganelliPubMed 38033871
- Scientific reports 13:183532023Hsa_circRNA_001676 accelerates the proliferation, migration and stemness in colorectal cancer through regulating miR-556-3p/G3BP2 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQin Hao,Miao Zhang,Yingcai Wu,Yuchen Guo,Yanling Zheng,Lijuan Wu,Li Feng,Zhenfei WangPubMed 37884630
- Nature communications 14:63412023Subventricular zone cytogenesis provides trophic support for neural repair in a mouse model of stroke.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichael R Williamson,Stephanie P Le,Ronald L Franzen,Nicole A Donlan,Jill L Rosow,Mathilda S Nicot-Cartsonis,Alexis Cervantes,Benjamin Deneen,Andrew K Dunn,Theresa A Jones,Michael R DrewPubMed 37816732
- Cancer biology & medicine 20:2023The inducible secreting TLR5 agonist, CBLB502, enhances the anti-tumor activity of CAR133-NK92 cells in colorectal cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaohui Wang,Wei Qiu,Haoyu Liu,Min He,Wei He,Zhan Li,Zhiqiang Wu,Xiang Xu,Ping ChenPubMed 37731205
- Frontiers in oncology 13:11513212023The role of BHLHE40 in clinical features and prognosis value of PDAC by comprehensive analysis and validation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChao Liu,Jiang Du,Jianwei Zheng,Ruizhe Zhang,Jialin Zhu,Bofan Xing,Lin Dong,Qianqian Zhou,Xiaofeng Yao,Song Gao,Yu Wang,Yu Ren,Xuan ZhouPubMed 37377917
- BMC complementary medicine and therapies 23:1832023Structural modification of resveratrol analogue exhibits anticancer activity against lung cancer stem cells via suppression of Akt signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSunisa Thongsom,Satapat Racha,Korrakod Petsri,Zin Zin Ei,Kittichate Visuttijai,Sohsuke Moriue,Masashi Yokoya,Pithi ChanvorachotePubMed 37270520
- Environmental toxicology 38:2002-20102023TRIM29 facilitates proliferation and malignancy of cholangiocarcinoma cells by activating MAPK and β-catenin pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Yang,Wenqing Bao,Haiyan Diao,Shaofei Yang,Lin Qiu,Chunjing Xu,Bin ZhaoPubMed 37219039
- Journal for immunotherapy of cancer 11:2023Intratumoral tertiary lymphoid structure (TLS) maturation is influenced by draining lymph nodes of lung cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMiao He,Qihua He,Xiuyu Cai,Jun Liu,Hongshen Deng,Feng Li,Ran Zhong,Yi Lu,Haoxin Peng,Xiangrong Wu,Zisheng Chen,Shen Lao,Caichen Li,Jianfu Li,Jianxing He,Wenhua LiangPubMed 37072348
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| ICC | IP | Flow Cyt | WB | IHC-P | ICC/IF | IHC-Fr | |
|---|---|---|---|---|---|---|---|
| Human | Expected | Expected | Expected | Tested | Expected | Expected | Expected |
| Mouse | Expected | Expected | Expected | Expected | Expected | Expected | Expected |
| Rat | Expected | Expected | Expected | Predicted | Expected | Expected | Expected |
ICC
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/30 | Notes - |
Species Mouse | Dilution info 1/30 | Notes - |
Species Rat | Dilution info 1/30 | Notes - |
Flow Cyt
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1-2 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-Fr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
May play a role in cell differentiation, proliferation and apoptosis (PubMed:24556617). Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner (PubMed:20818439).
Alternative names
CD133, PROML1, MSTP061, PROM1, Prominin-1, Antigen AC133, Prominin-like protein 1
Recommended products
Anti-CD133 antibody - Stem Cell Marker is a rabbit polyclonal antibody that is used to detect CD133 in Flow cytometry, ICC, ICC/IF, IHC-Fr, IHC-P, IP, Western blot. Suitable for Human, Mouse, Rat samples.
- Stem Cell Marker
- Cited in over 260 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. You may also be interested in our alternative recombinant antibody, ab284389.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-CD133 antibody - Stem Cell Marker (ab19898) is a rabbit polyclonal antibody and is validated for use in Flow Cyt, ICC, ICC/IF, IHC-Fr, IHC-P, IP, WB in human, mouse samples.
Anti-CD133 antibody - Stem Cell Marker (ab19898) has been cited over 264 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-CD133 antibody - Stem Cell Marker (ab19898) has high sensitivity and specificity.
Anti-CD133 antibody - Stem Cell Marker (ab19898) has 26 independent reviews from customers.
Anti-CD133 antibody - Stem Cell Marker (ab19898) specifically detects CD133 (UniProt ID: O43490; Molecular weight: 95kDa) and is sold in a 100 µg selling size.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD133 also known as Prominin-1 is a transmembrane glycoprotein with a molecular weight of approximately 120 kDa. This protein stands out due to its expression in various stem and progenitor cells including those of the neural hematopoietic and epithelial lineages. CD133 is recognized for its distinct five transmembrane domains and two large extracellular loops. Researchers commonly use CD133 as a cell surface marker to identify stem and progenitor cells. The significance of CD133 extends to immunohistochemistry (IHC) and Western blot applications where it is a popular target for detection and analysis.
- Biological function summary
CD133 is involved in maintaining the cellular architecture and signaling processes. It plays a role in cellular differentiation and proliferation while acting as a part of larger protein complexes on the cell surface. This protein is notable as it contributes significantly to the regulation of stem cell properties supporting the growth and repair in various tissues. Anti-CD133 antibodies are valuable tools in studying these functions due to their specificity in targeting this marker.
- Pathways
CD133 participates in cellular signaling and transport pathways vital for intercellular communication and homeostasis. It interacts within pathways related to cell polarity and asymmetric division both essential for tissue development and maintenance. CD133 is associated with proteins like CD133/1 and CD133/2 which further highlights its involvement in these complex biological networks and on pathways such as the Wnt signaling pathway which regulates gene expression associated with cell proliferation and differentiation.
- Associated diseases and disorders
Several studies indicate a strong relationship between CD133 expression and certain cancers such as glioblastoma and colorectal cancer. In these cases CD133 serves not only as a marker but also as an indicator of the presence of cancer stem cells which are capable of tumor initiation and maintenance. Furthermore researchers have identified connections between CD133 and other proteins such as c-Met (cMab) within these pathological contexts suggesting interactions that could contribute to disease progression and offering potential targets for therapeutic intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
Western blot - Anti-CD133 antibody - Stem Cell Marker (ab19898)
Lanes 1 - 2: Merged signal (red and green). Green - ab19898 observed at 97 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.ab19898 was shown to react with CD133 in western blot. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab19898 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at 1 μg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-CD133 antibody - Stem Cell Marker (ab19898) at 1 µg/mL
Lane 1: Caco 2 cell lysate at 20 µg
Lane 2: HeLa cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 97 kDa
Observed band size: 97 kDa
Immunoprecipitation - Anti-CD133 antibody - Stem Cell Marker (ab19898)
Immunoprecipitation side-by-side comparison with the recombinant multiclonal antibody Anti-CD133 antibody [RM1002] - Stem Cell Marker ab278053
This immunoprecipitation image is a comparison between ab19898 and the alternative recombinant multiclonal antibody Anti-CD133 antibody [RM1002] - Stem Cell Marker ab278053.
Left side - Recombinant multiclonal to CD133 - Anti-CD133 antibody [RM1002] - Stem Cell Marker ab278053
CD133 was immunoprecipitated from 0.35 mg Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate with Anti-CD133 antibody [RM1002] - Stem Cell Marker ab278053 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-CD133 antibody [RM1002] - Stem Cell Marker ab278053 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Caco-2 whole cell lysate 10 μg.
Lane 2: Anti-CD133 antibody [RM1002] - Stem Cell Marker ab278053 IP in Caco-2 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CD133 antibody [RM1002] - Stem Cell Marker ab278053 in Caco-2 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
Right side - Polyclonal antibody to [CD133] - ab19898
Same testing conditions as Anti-CD133 antibody [RM1002] - Stem Cell Marker ab278053.
Why choose a recombinant antibody?
Research with confidence - consistent and reproducible results with every batch
Long-term and scalable supply - powered by recombinant technology for fast production
Success from the first experiment - confirmed specificity through extensive validation
Ethical standards compliant - production is animal-freeAll lanes: Immunoprecipitation - Anti-CD133 antibody - Stem Cell Marker (ab19898)
Predicted band size: 97 kDa
Western blot - Anti-CD133 antibody - Stem Cell Marker (ab19898)
Western blot side-by-side comparison with the recombinant multiclonal antibody Anti-CD133 antibody [RM1002] - Stem Cell Marker ab278053
This Western blot image is a comparison between ab19898 and the alternative recombinant multiclonal antibody Anti-CD133 antibody [RM1002] - Stem Cell Marker ab278053.
Left side - Recombinant multiclonal to CD133 - Anti-CD133 antibody [RM1002] - Stem Cell Marker ab278053
All lanes : Anti-CD133 antibody [RM1002] (Anti-CD133 antibody [RM1002] - Stem Cell Marker ab278053) at 1/1000 dilution
Lane 1 : Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HEK-293T (Human embryonic kidney epithelial cell) whole cell lysate
Lane 3 : NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate
Lane 4 : HT-29 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 μg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 97 kDa
Observed band size: 120 kDa
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 26 seconds.
Right side - Polyclonal antibody to [CD133] - ab19898
Same testing conditions as Anti-CD133 antibody [RM1002] - Stem Cell Marker ab278053.
Why choose a recombinant antibody?
Research with confidence - consistent and reproducible results with every batch
Long-term and scalable supply - powered by recombinant technology for fast production
Success from the first experiment - confirmed specificity through extensive validation
Ethical standards compliant - production is animal-freeAll lanes: Western blot - Anti-CD133 antibody - Stem Cell Marker (ab19898)
Predicted band size: 97 kDa
Western blot - Anti-CD133 antibody - Stem Cell Marker (ab19898)
ab19898 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
All lanes: Western blot - Anti-CD133 antibody - Stem Cell Marker (ab19898) at 1 µg/mL
Lane 1: MEL-1 (Human embryonic stem cell, male cell line) Whole Cell Lysate (ab27198) at 10 µg
Lane 2: Western blot - HT-29 whole cell lysate (HT-29 whole cell lysate ab3952) at 10 µg
Lane 3: Caco-2 whole cell lysate (ab3950) at 10 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 97 kDa
Observed band size: 110 kDa
Western blot - Anti-CD133 antibody - Stem Cell Marker (ab19898)
Anti-CD133 antibody ab19898 detects a band corresponding to the expected size of CD133 in Human Embryonic Stem Cell Lysate using Western Blotting. It is likely that the band runs higher than the predicted MW of CD133 due to glycosylation of CD133. In mouse neural stem cell and mouse embryonic stem cell lysate this ~110 kDa band was not detected but a reproducible band of ~30 kDa was seen. This could represent a cleavage product but it may also be that the ~30 kDa band represents non-specific binding by ab19898. However, ab19898 has been shown to recognise mouse neural stem cells using ICC. Blocking using the immunising peptide removed both of these bands.
All lanes: Western blot - Anti-CD133 antibody - Stem Cell Marker (ab19898) at 1 µg/mL
Lane 1: Human Embryonic Stem Cell Lysate at 20 µg
Lane 2: Human Embryonic Stem Cell Lysate at 20 µg with CD133 peptide (ab20651)
Predicted band size: 97 kDa
Immunocytochemistry/ Immunofluorescence - Anti-CD133 antibody - Stem Cell Marker (ab19898)
ab19898 staining CD133 in Caco-2 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab19898 at 1µg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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