Rabbit Monoclonal CD134 / OX40L receptor antibody. Suitable for ICC, IP, Flow Cyt, WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human TNFRSF4.
Images
![Immunoprecipitation - Anti-CD134 / OX40L receptor antibody [BLR042F] (AB243898), expandable thumbnail](https://content.abcam.com/products/images/cd134-ox40l-receptor-antibody-blr042f-ab243898--immunoprecipitation-img154060.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-CD134 / OX40L receptor antibody [BLR042F] (AB243898)")
![Immunocytochemistry - Anti-CD134 / OX40L receptor antibody [BLR042F] (AB243898), expandable thumbnail](https://content.abcam.com/products/images/cd134-ox40l-receptor-antibody-blr042f-ab243898--immunocytochemistry-img129058.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry - Anti-CD134 / OX40L receptor antibody [BLR042F] (AB243898)")
![Western blot - Anti-CD134 / OX40L receptor antibody [BLR042F] (AB243898), expandable thumbnail](https://content.abcam.com/products/images/cd134-ox40l-receptor-antibody-blr042f-ab243898--western-blot-img100276.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CD134 / OX40L receptor antibody [BLR042F] (AB243898)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [BLR042F] (AB243898), expandable thumbnail](https://content.abcam.com/products/images/cd134-ox40l-receptor-antibody-blr042f-ab243898--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img43115.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [BLR042F] (AB243898)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [BLR042F] (AB243898), expandable thumbnail](https://content.abcam.com/products/images/cd134-ox40l-receptor-antibody-blr042f-ab243898--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img167895.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [BLR042F] (AB243898)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- Recombinant Fragment Protein within Human TNFRSF4. The exact immunogen used to generate this antibody is proprietary information. Database link P43489
Reactivity data
Select an application| ICC | IP | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Expected | Tested | Tested |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/100.00000 | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
15 products for Alternative Product
Target data
Function
Receptor for TNFSF4/OX40L/GP34. Is a costimulatory molecule implicated in long-term T-cell immunity.(Microbial infection) Acts as a receptor for human herpesvirus 6B/HHV-6B.
Alternative names
Tumor necrosis factor receptor superfamily member 4, ACT35 antigen, OX40L receptor, TAX transcriptionally-activated glycoprotein 1 receptor, TNFRSF4, TXGP1L
Recommended products
Rabbit Monoclonal CD134 / OX40L receptor antibody. Suitable for ICC, IP, Flow Cyt, WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human TNFRSF4.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human TNFRSF4. The exact immunogen used to generate this antibody is proprietary information. Database link P43489
- Clone number
BLR042F
- Concentration
- Purification notes
Recombinant antibody was purified from cell culture supernatant.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
This product is sold under License from Bethyl Laboratories, Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD134 also known as OX40 or TNFRSF4 is a receptor that plays an essential role in immune response regulation. This receptor is part of the tumor necrosis factor receptor (TNFR) superfamily and has a molecular mass of approximately 50 kDa. CD134 is expressed on activated T cells particularly CD4+ T cells and is present on various other immune cells under specific conditions. The receptor binds to the OX40L its ligand which is expressed on antigen-presenting cells such as dendritic cells and B cells.
- Biological function summary
CD134 influences T cell proliferation survival and memory formation being an important component of the immune system's adaptive response. OX40L engagement with CD134 enhances the activation and survival of T cells promoting long-term immunity. This interaction does not form part of a larger complex but functions as a direct signaling mechanism that augments T cell receptor (TCR) signaling leading to increased cytokine production and sustained immune responses essential for controlling infections and cancer.
- Pathways
CD134 signaling intersects with the nuclear factor-kappa B (NF-κB) and phosphoinositide 3-kinase (PI3K) pathways. These pathways play significant roles in regulating immune responses and cell survival. Through the NF-κB pathway CD134 influences gene transcription related to immune activation. It also connects with other immune regulatory proteins such as CD28 which further potentiates T cell responses and enhances effector functions.
- Associated diseases and disorders
CD134 has connections to autoimmune diseases and cancers. Overactivity of the CD134-OX40L pathway can lead to exacerbated autoimmune conditions such as lupus. This receptor has also been implicated in tumor immunity where its activation enhances the anti-tumor response of T cells. CD134 interacts with CTLA-4 another immune checkpoint protein playing a role in balancing immune activation and regulation within these disease contexts.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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5 product images
![Immunoprecipitation - Anti-CD134 / OX40L receptor antibody [BLR042F] (ab243898), expandable thumbnail](https://content.abcam.com/products/images/cd134-ox40l-receptor-antibody-blr042f-ab243898--immunoprecipitation-img154060.jpg "Immunoprecipitation - Anti-CD134 / OX40L receptor antibody [BLR042F] (ab243898)")
Immunoprecipitation - Anti-CD134 / OX40L receptor antibody [BLR042F] (ab243898)
CD134 / OX40L was immunoprecipitated from 1.0 mg MJ whole cell lysate with ab243898 at 20 µl per reaction. Western blot was performed on the immunoprecipitate using ab243898 at 1/1000 dilution. Lane 1: ab243898 IP in MJ whole cell lysate.
Lane 2: Control IgG in MJ whole cell lysate.
Detection: Chemiluminescence with an exposure time of 30 seconds.
All lanes: Immunoprecipitation - Anti-CD134 / OX40L receptor antibody [BLR042F] (ab243898)
Predicted band size: 29 kDa
![Immunocytochemistry - Anti-CD134 / OX40L receptor antibody [BLR042F] (ab243898), expandable thumbnail](https://content.abcam.com/products/images/cd134-ox40l-receptor-antibody-blr042f-ab243898--immunocytochemistry-img129058.jpg "Immunocytochemistry - Anti-CD134 / OX40L receptor antibody [BLR042F] (ab243898)")
Immunocytochemistry - Anti-CD134 / OX40L receptor antibody [BLR042F] (ab243898)
Formalin-fixed, paraffin-embedded MJ cells labeling CD134 / OX40L using ab243898 at 1/250 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.
![Western blot - Anti-CD134 / OX40L receptor antibody [BLR042F] (ab243898), expandable thumbnail](https://content.abcam.com/products/images/cd134-ox40l-receptor-antibody-blr042f-ab243898--western-blot-img100276.jpg "Western blot - Anti-CD134 / OX40L receptor antibody [BLR042F] (ab243898)")
Western blot - Anti-CD134 / OX40L receptor antibody [BLR042F] (ab243898)
Western blot analysis using ab243898 at 1/1000 dilution.
Lane 1: HEK-293T whole cell lysate (50 µg).
Lane 2: MCF7 whole cell lysate (50 µg).
Lane 3: HepG2 whole cell lysate (50 µg).
Lane 4: A549 whole cell lysate (50 µg).
Lane 5: SW 620 whole cell lysate (50 µg).
Lane 6: SK whole cell lysate (50 µg).
Lane 7: Jurkat whole cell lysate (50 µg).
Lane 8: TCMK-1 whole cell lysate (50 µg).
Lane 9: NIH/3T3 whole cell lysate (50 µg).
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 10 seconds.
All lanes: Western blot - Anti-CD134 / OX40L receptor antibody [BLR042F] (ab243898)
Predicted band size: 29 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [BLR042F] (ab243898), expandable thumbnail](https://content.abcam.com/products/images/cd134-ox40l-receptor-antibody-blr042f-ab243898--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img43115.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [BLR042F] (ab243898)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [BLR042F] (ab243898)
Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for CD134 / OX40L using ab243898 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [BLR042F] (ab243898), expandable thumbnail](https://content.abcam.com/products/images/cd134-ox40l-receptor-antibody-blr042f-ab243898--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img167895.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [BLR042F] (ab243898)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [BLR042F] (ab243898)
Immunohistochemical analysis of formalin-fixed, parafin-embedded human breast carcinoma tissue labeling CD134 / OX40L with ab243898 at 1/250 dilution, followed by HRP-conjugated Goat Anti-Rabbit IgG secondary antibody. Counter stained with DAPI. Opal staining.
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