AB76000

Anti-CD134 / OX40L receptor antibody [EPR17Y]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(2 Publications)

Rabbit Recombinant Monoclonal CD134 / OX40L receptor antibody. Suitable for Flow Cyt, WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

CD134, TXGP1L, TNFRSF4, Tumor necrosis factor receptor superfamily member 4, ACT35 antigen, OX40L receptor, TAX transcriptionally-activated glycoprotein 1 receptor

4 Images

Flow Cytometry - Anti-CD134 / OX40L receptor antibody [EPR17Y] (AB76000)

Flow Cyt

Lab

Flow Cytometry - Anti-CD134 / OX40L receptor antibody [EPR17Y] (AB76000)

Flow cytometry analysis of Human PBMC treated with 1© : g/ml PHA for 3 days cells labelling CD134 / OX40L receptor with ab76000 at 1/1200 dilution (1.05 μg/ml) (Red). Dylight488 (ab98462) was used as the secondary antibody at 1/2000 dilution.

Unknown

Western blot - Anti-CD134 / OX40L receptor antibody [EPR17Y] (AB76000)

Blocking and diluting buffer 5% NFDM/TBST

All lanes:

Western blot - Anti-CD134 / OX40L receptor antibody [EPR17Y] (ab76000) at 1/1000 dilution

All lanes:

Human tonsil tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 29 kDa

Observed band size: 45 kDa

false

Unknown

Western blot - Anti-CD134 / OX40L receptor antibody [EPR17Y] (AB76000)

Blocking and diluting buffer 5% NFDM/TBST

All lanes:

Western blot - Anti-CD134 / OX40L receptor antibody [EPR17Y] (ab76000) at 1/5000 dilution

Lane 1:

Human lymph node tissue lysate at 10 µg

Lane 2:

Human spleen tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 29 kDa

Observed band size: 45 kDa

false

Unknown

Western blot - Anti-CD134 / OX40L receptor antibody [EPR17Y] (AB76000)

Note : the actual molecular weight is larger than the predicted due to glycoslyation.

All lanes:

Western blot - Anti-CD134 / OX40L receptor antibody [EPR17Y] (ab76000) at 1/500 dilution

Lane 1:

PBMC cell lysates, untreated at 10 µg

Lane 2:

PBMC cell lysates, treated with TPA at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 29 kDa,32 kDa

Observed band size: 45 kDa

false

Carrier free

Anti-CD134 / OX40L receptor antibody [EPR17Y] - BSA and Azide free (Detector)
Carrier free

Anti-CD134 / OX40L receptor antibody [EPR17Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17Y

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.19% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD134 also known as OX40 or TNFRSF4 is a receptor that plays an essential role in immune response regulation. This receptor is part of the tumor necrosis factor receptor (TNFR) superfamily and has a molecular mass of approximately 50 kDa. CD134 is expressed on activated T cells particularly CD4+ T cells and is present on various other immune cells under specific conditions. The receptor binds to the OX40L its ligand which is expressed on antigen-presenting cells such as dendritic cells and B cells.

Biological function summary

CD134 influences T cell proliferation survival and memory formation being an important component of the immune system's adaptive response. OX40L engagement with CD134 enhances the activation and survival of T cells promoting long-term immunity. This interaction does not form part of a larger complex but functions as a direct signaling mechanism that augments T cell receptor (TCR) signaling leading to increased cytokine production and sustained immune responses essential for controlling infections and cancer.

Pathways

CD134 signaling intersects with the nuclear factor-kappa B (NF-κB) and phosphoinositide 3-kinase (PI3K) pathways. These pathways play significant roles in regulating immune responses and cell survival. Through the NF-κB pathway CD134 influences gene transcription related to immune activation. It also connects with other immune regulatory proteins such as CD28 which further potentiates T cell responses and enhances effector functions.

CD134 has connections to autoimmune diseases and cancers. Overactivity of the CD134-OX40L pathway can lead to exacerbated autoimmune conditions such as lupus. This receptor has also been implicated in tumor immunity where its activation enhances the anti-tumor response of T cells. CD134 interacts with CTLA-4 another immune checkpoint protein playing a role in balancing immune activation and regulation within these disease contexts.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Receptor for TNFSF4/OX40L/GP34. Is a costimulatory molecule implicated in long-term T-cell immunity.. (Microbial infection) Acts as a receptor for human herpesvirus 6B/HHV-6B.

See full target information TNFRSF4

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 12:3242-3248 PubMed27882144

2016

Effect of cyclosporin A intervention on the immunological mechanisms of coronary heart disease and restenosis.

Applications

IHC-P

Species

Rabbit

Xuan Wang,Yue-Cheng Hu,Ru-Yan Zhang,Dong-Xia Jin,Yuan Jiang,He-Nan Zhang,Hong-Liang Cong

The Journal of experimental medicine 210:1743-59 PubMed23897980

2013

Inherited human OX40 deficiency underlying classic Kaposi sarcoma of childhood.

Applications

WB, Flow Cyt

Species

Human, Human

Minji Byun,Cindy S Ma,Arzu Akçay,Vincent Pedergnana,Umaimainthan Palendira,Jinjong Myoung,Danielle T Avery,Yifang Liu,Avinash Abhyankar,Lazaro Lorenzo,Monika Schmidt,Hye Kyung Lim,Olivier Cassar,Melanie Migaud,Flore Rozenberg,Nur Canpolat,Gönül Aydogan,Bernhard Fleckenstein,Jacinta Bustamante,Capucine Picard,Antoine Gessain,Emmanuelle Jouanguy,Ethel Cesarman,Martin Olivier,Philippe Gros,Laurent Abel,Michael Croft,Stuart G Tangye,Jean-Laurent Casanova

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD134 / OX40L receptor antibody [EPR17Y]

Flow Cytometry - Anti-CD134 / OX40L receptor antibody [EPR17Y] (AB76000)

Western blot - Anti-CD134 / OX40L receptor antibody [EPR17Y] (AB76000)

Western blot - Anti-CD134 / OX40L receptor antibody [EPR17Y] (AB76000)

Western blot - Anti-CD134 / OX40L receptor antibody [EPR17Y] (AB76000)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise