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AB259689

Anti-CD134 / OX40L receptor antibody [EPR22229-112] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal CD134 / OX40L receptor antibody. Carrier free. Suitable for sELISA and reacts with Mouse, Rat samples.

View Alternative Names

CD134, Ox40, Txgp1, Tnfrsf4, Tumor necrosis factor receptor superfamily member 4, OX40 antigen, OX40L receptor

2 Images
Sandwich ELISA - Anti-CD134 / OX40L receptor antibody [EPR22229-112] - BSA and Azide free (Capture) (AB259689)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-CD134 / OX40L receptor antibody [EPR22229-112] - BSA and Azide free (Capture) (AB259689)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab255725).

Sandwich ELISA - Anti-CD134 / OX40L receptor antibody [EPR22229-112] - BSA and Azide free (Capture) (AB259689)
  • sELISA

Unknown

Sandwich ELISA - Anti-CD134 / OX40L receptor antibody [EPR22229-112] - BSA and Azide free (Capture) (AB259689)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab253218).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22229-112

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab259689 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse OX40. The recommended pair for sandwich ELISA is:
Capture: ab259689, Mouse OX40 Capture Antibody (unconjugated)
Detector: ab259690, Mouse OX40 Detector Antibody (unconjugated)
The reference range value is 62.5 - 4000 pg/ml.

Or alternatively, for Rat, the recommended pair for sandwich ELISA is:
Capture: ab259689, Rat OX40 Capture Antibody (unconjugated)
Detector: ab259768, Rat OX40 Detector Antibody (unconjugated)
The reference range value is 62.5 - 4000 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD134 also known as OX40 or TNFRSF4 is a receptor that plays an essential role in immune response regulation. This receptor is part of the tumor necrosis factor receptor (TNFR) superfamily and has a molecular mass of approximately 50 kDa. CD134 is expressed on activated T cells particularly CD4+ T cells and is present on various other immune cells under specific conditions. The receptor binds to the OX40L its ligand which is expressed on antigen-presenting cells such as dendritic cells and B cells.
Biological function summary

CD134 influences T cell proliferation survival and memory formation being an important component of the immune system's adaptive response. OX40L engagement with CD134 enhances the activation and survival of T cells promoting long-term immunity. This interaction does not form part of a larger complex but functions as a direct signaling mechanism that augments T cell receptor (TCR) signaling leading to increased cytokine production and sustained immune responses essential for controlling infections and cancer.

Pathways

CD134 signaling intersects with the nuclear factor-kappa B (NF-κB) and phosphoinositide 3-kinase (PI3K) pathways. These pathways play significant roles in regulating immune responses and cell survival. Through the NF-κB pathway CD134 influences gene transcription related to immune activation. It also connects with other immune regulatory proteins such as CD28 which further potentiates T cell responses and enhances effector functions.

CD134 has connections to autoimmune diseases and cancers. Overactivity of the CD134-OX40L pathway can lead to exacerbated autoimmune conditions such as lupus. This receptor has also been implicated in tumor immunity where its activation enhances the anti-tumor response of T cells. CD134 interacts with CTLA-4 another immune checkpoint protein playing a role in balancing immune activation and regulation within these disease contexts.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for TNFSF4/OX40L/GP34. Is a costimulatory molecule implicated in long-term T-cell immunity (By similarity).
See full target information Tnfrsf4
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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