Rabbit Recombinant Monoclonal CD134 / OX40L receptor antibody. Suitable for IP, Flow Cyt, WB, IHC-P and reacts with Rat, Mouse samples. Cited in 3 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
IP | Flow Cyt | WB | ICC/IF | IHC-Fr | IHC-P | |
---|---|---|---|---|---|---|
Mouse | Expected | Tested | Tested | Not recommended | Not recommended | Tested |
Rat | Tested | Tested | Tested | Not recommended | Not recommended | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info 1/30 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/500 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Select an associated product type
Receptor for TNFSF4/OX40L/GP34. Is a costimulatory molecule implicated in long-term T-cell immunity (By similarity).
Ox40, Txgp1, Tnfrsf4, Tumor necrosis factor receptor superfamily member 4, OX40 antigen, OX40L receptor
Rabbit Recombinant Monoclonal CD134 / OX40L receptor antibody. Suitable for IP, Flow Cyt, WB, IHC-P and reacts with Rat, Mouse samples. Cited in 3 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR22229-5
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Flow cytometric analysis of mouse primary splenocytes treated with 2.5μg/ml concanavalin A for 72h labeling CD134 / OX40L with ab229021 at 1/500 dilution (Right) compared to cells stained with Alexa Fluor® 647-conjugated CD3 and rabbit IgG (Left). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/2000 dilution.
Data shown were gated on viable cells.
Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling CD134 / OX40L using ab229021 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on T cells in rat spleen (PMID: 15259024; PMID: 10233901). Counterstained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins.
The section was incubated with ab229021 for 10 mins at 37°C. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.
CD134 / OX40L was immunoprecipitated from 0.35 mg rat splenocytes treated with 2.5μg/ml concanavalin A for 72h lysate using ab229021 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab229021 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at a 1/5000 dilution.
Lane 1: Rat splenocytes treated with 2.5μg/ml concanavalin A for 72h lysate 10 μg (input)
Lane 2: ab229021 IP in Rat splenocytes treated with 2.5μg/ml concanavalin A for 72h lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab229021 in Rat splenocytes treated with 2.5μg/ml concanavalin A for 72h lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 20 seconds.
All lanes: Immunoprecipitation - Anti-CD134 / OX40L receptor antibody [EPR22229-5] (ab229021)
Predicted band size: 29 kDa
Flow cytometric analysis of rat primary splenocytes treated with 2.5μg/ml concanavalin A for 72h labeling CD134 / OX40L with ab229021 at 1/500 dilution (Right) compared to cells stained with Alexa Fluor® 647-conjugated CD3 and rabbit IgG (Left). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/2000 dilution.
Data shown were gated on viable cells.
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD134 / OX40L using ab229021 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on T cells in periarterial lymphatic sheath of mouse spleen (PMID: 15259024; PMID: 10233901). Counterstained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins.
The section was incubated with ab229021 for 10 mins at 37°C. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
CD134 / OX40L is a glycosylated protein and its expression is increased after concanavalin A treatment. PMID 23897980; PMID 17609274
All lanes: Western blot - Anti-CD134 / OX40L receptor antibody [EPR22229-5] (ab229021) at 1/1000 dilution
Lane 1: Untreated mouse splenocytes lysate at 20 µg
Lane 2: Mouse splenocytes treated with 2.5ug/ml concanavalin A for 72h lysate at 20 µg
Lane 3: Untreated rat splenocytes lysate at 20 µg
Lane 4: Rat splenocytes treated with 2.5ug/ml concanavalin A for 72h lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 29 kDa
Observed band size: 50 kDa
Exposure time: 3min
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