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Rabbit Recombinant Monoclonal CD134 / OX40L receptor antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 1 publication.

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Images

Western blot - Anti-CD134 / OX40L receptor antibody [EPR23000-42] (AB264466), expandable thumbnail
  • Western blot - Anti-CD134 / OX40L receptor antibody [EPR23000-42] (AB264466), expandable thumbnail
  • Immunoprecipitation - Anti-CD134 / OX40L receptor antibody [EPR23000-42] (AB264466), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [EPR23000-42] (AB264466), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [EPR23000-42] (AB264466), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/30

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/50

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500

Notes

-

Tested
Tested

Species

Human

Dilution info

1/4000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

Receptor for TNFSF4/OX40L/GP34. Is a costimulatory molecule implicated in long-term T-cell immunity.(Microbial infection) Acts as a receptor for human herpesvirus 6B/HHV-6B.

Alternative names

Tumor necrosis factor receptor superfamily member 4, ACT35 antigen, OX40L receptor, TAX transcriptionally-activated glycoprotein 1 receptor, TNFRSF4, TXGP1L

Recommended products

Rabbit Recombinant Monoclonal CD134 / OX40L receptor antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 1 publication.

Alternative names

Tumor necrosis factor receptor superfamily member 4, ACT35 antigen, OX40L receptor, TAX transcriptionally-activated glycoprotein 1 receptor, TNFRSF4, TXGP1L

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR23000-42

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD134 also known as OX40 or TNFRSF4 is a receptor that plays an essential role in immune response regulation. This receptor is part of the tumor necrosis factor receptor (TNFR) superfamily and has a molecular mass of approximately 50 kDa. CD134 is expressed on activated T cells particularly CD4+ T cells and is present on various other immune cells under specific conditions. The receptor binds to the OX40L its ligand which is expressed on antigen-presenting cells such as dendritic cells and B cells.

Biological function summary

CD134 influences T cell proliferation survival and memory formation being an important component of the immune system's adaptive response. OX40L engagement with CD134 enhances the activation and survival of T cells promoting long-term immunity. This interaction does not form part of a larger complex but functions as a direct signaling mechanism that augments T cell receptor (TCR) signaling leading to increased cytokine production and sustained immune responses essential for controlling infections and cancer.

Pathways

CD134 signaling intersects with the nuclear factor-kappa B (NF-κB) and phosphoinositide 3-kinase (PI3K) pathways. These pathways play significant roles in regulating immune responses and cell survival. Through the NF-κB pathway CD134 influences gene transcription related to immune activation. It also connects with other immune regulatory proteins such as CD28 which further potentiates T cell responses and enhances effector functions.

Associated diseases and disorders

CD134 has connections to autoimmune diseases and cancers. Overactivity of the CD134-OX40L pathway can lead to exacerbated autoimmune conditions such as lupus. This receptor has also been implicated in tumor immunity where its activation enhances the anti-tumor response of T cells. CD134 interacts with CTLA-4 another immune checkpoint protein playing a role in balancing immune activation and regulation within these disease contexts.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-CD134 / OX40L receptor antibody [EPR23000-42] (ab264466), expandable thumbnail

    Western blot - Anti-CD134 / OX40L receptor antibody [EPR23000-42] (ab264466)

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This blot was developed using a higher sensitivity ECL substrate.

    The molecular weight observed is consistent with what has been described in the literature (PMID:9108415, 23897980).

    All lanes: Western blot - Anti-CD134 / OX40L receptor antibody [EPR23000-42] (ab264466) at 1/1000 dilution

    Lane 1: Human thymus tissue lysate at 10 µg

    Lane 2: Human lymph node at 10 µg

    Secondary

    All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Predicted band size: 29 kDa

    Observed band size: 50 kDa

  • Western blot - Anti-CD134 / OX40L receptor antibody [EPR23000-42] (ab264466), expandable thumbnail

    Western blot - Anti-CD134 / OX40L receptor antibody [EPR23000-42] (ab264466)

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 37 seconds.

    The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID:9108415,23897980).

    Negative control: Jurkat (PMID:9108415).

    All lanes: Western blot - Anti-CD134 / OX40L receptor antibody [EPR23000-42] (ab264466) at 1/1000 dilution

    Lane 1: Untreated human PBMC, whole cell lysate at 20 µg

    Lane 2: Human PBMC treated with 10 μg/ml phytohaemagglutinin (PHA) for 2 days, whole cell lysate at 20 µg

    Lane 3: Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/500 dilution

    Predicted band size: 29 kDa

    Observed band size: 37 kDa, 50 kDa

  • Immunoprecipitation - Anti-CD134 / OX40L receptor antibody [EPR23000-42] (ab264466), expandable thumbnail

    Immunoprecipitation - Anti-CD134 / OX40L receptor antibody [EPR23000-42] (ab264466)

    CD134 / OX40L receptor was immunoprecipitated from 0.35 mg Human thymus lysate with ab264466 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab264466 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/1000 dilution.

    Lane 1: Human thymus lysate 10ug

    Lane 2: ab264466 IP in Human thymus lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab264466 in Human thymus lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 min

    All lanes: Immunoprecipitation - Anti-CD134 / OX40L receptor antibody [EPR23000-42] (ab264466)

    Predicted band size: 29 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [EPR23000-42] (ab264466), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [EPR23000-42] (ab264466)

    Immunohistochemical analysis of paraffin-embedded human Hodgkin's lymphoma tissue labeling CD134 / OX40L receptor with ab264466 at 1/4000 dilution (0.13 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on human Hodgkin's lymphoma (PMID/8547142). Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [EPR23000-42] (ab264466), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [EPR23000-42] (ab264466)

    Immunohistochemical analysis of paraffin-embedded human thymus tissue labeling CD134 / OX40L with ab264466 at 1/4000 dilution (0.13 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on T cells of human thymus (PMID/8547142). Counterstained with Hematoxylin.

    Secondary antibody only control/ Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Flow Cytometry (Intracellular) - Anti-CD134 / OX40L receptor antibody [EPR23000-42] (ab264466), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-CD134 / OX40L receptor antibody [EPR23000-42] (ab264466)

    Intracellular flow cytometric analysis of 2% Paraformaldehyde fixed, 0.1% Tween-20 permeabilized Human peripheral blood mononuclear cell (PBMC) treated with 10 μg/ml phytohemagglutinin (PHA) for 2days labelling CD132 / OX40L receptor with ab264466 at 1/500 dilution 9Right) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) control (Left). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

    Cells were surface stained with anti-CD3 conjugated to Alexa Fluor® 647. Then fixed with 2% PFA followed by intracellularly stained with rabbit IgG (Left) or ab264466 (Right). (PMID: 9108415, 17609274).

  • Immunocytochemistry/ Immunofluorescence - Anti-CD134 / OX40L receptor antibody [EPR23000-42] (ab264466), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CD134 / OX40L receptor antibody [EPR23000-42] (ab264466)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized human PBMCs labelling CD34 / OX40L with ab264466 at 1/50 (10 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing positive staining in human PBMC treated with phytohaemagglutinin (PHA) (10 μg/ml for 2 days) (PMID: 9108415). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 ug/ml) dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [EPR23000-42] (ab264466), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [EPR23000-42] (ab264466)

    Tissue Microarrays stained for "Anti-CD134 / OX40L receptor antibody [EPR23000-42]" using "ab264466"in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). The sections were incubated with ab264466 at +4°C overnight followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

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