Rabbit Recombinant Monoclonal CD134 / OX40L receptor antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples.
Images
![Immunoprecipitation - Anti-CD134 / OX40L receptor antibody [EPR23000-42] - BSA and Azide free (AB264470), expandable thumbnail](https://content.abcam.com/products/images/cd134-ox40l-receptor-antibody-epr23000-42-bsa-and-azide-free-ab264470--immunoprecipitation-img3592.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-CD134 / OX40L receptor antibody [EPR23000-42] - BSA and Azide free (AB264470)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [EPR23000-42] - BSA and Azide free (AB264470), expandable thumbnail](https://content.abcam.com/products/images/cd134-ox40l-receptor-antibody-epr23000-42-bsa-and-azide-free-ab264470--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104649.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [EPR23000-42] - BSA and Azide free (AB264470)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [EPR23000-42] - BSA and Azide free (AB264470), expandable thumbnail](https://content.abcam.com/products/images/cd134-ox40l-receptor-antibody-epr23000-42-bsa-and-azide-free-ab264470--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img84042.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [EPR23000-42] - BSA and Azide free (AB264470)")
![Flow Cytometry (Intracellular) - Anti-CD134 / OX40L receptor antibody [EPR23000-42] - BSA and Azide free (AB264470), expandable thumbnail](https://content.abcam.com/products/images/cd134-ox40l-receptor-antibody-epr23000-42-bsa-and-azide-free-ab264470--flow-cytometry-intracellular-img17655.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-CD134 / OX40L receptor antibody [EPR23000-42] - BSA and Azide free (AB264470)")
![Immunocytochemistry/ Immunofluorescence - Anti-CD134 / OX40L receptor antibody [EPR23000-42] - BSA and Azide free (AB264470), expandable thumbnail](https://content.abcam.com/products/images/cd134-ox40l-receptor-antibody-epr23000-42-bsa-and-azide-free-ab264470--immunocytochemistry-immunofluorescence-img91873.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CD134 / OX40L receptor antibody [EPR23000-42] - BSA and Azide free (AB264470)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|---|
| Human | Tested | Expected | Tested | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
6 products for Alternative Product
8 products for Alternative Version
Target data
Function
Receptor for TNFSF4/OX40L/GP34. Is a costimulatory molecule implicated in long-term T-cell immunity.(Microbial infection) Acts as a receptor for human herpesvirus 6B/HHV-6B.
Alternative names
TXGP1L, TNFRSF4, TXGP1L, Tumor necrosis factor receptor superfamily member 4, ACT35 antigen, OX40L receptor, TAX transcriptionally-activated glycoprotein 1 receptor
Recommended products
Rabbit Recombinant Monoclonal CD134 / OX40L receptor antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR23000-42
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab264470 is the carrier-free version of Anti-CD134 / OX40L receptor antibody [EPR23000-42] ab264466.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD134 also known as OX40 or TNFRSF4 is a receptor that plays an essential role in immune response regulation. This receptor is part of the tumor necrosis factor receptor (TNFR) superfamily and has a molecular mass of approximately 50 kDa. CD134 is expressed on activated T cells particularly CD4+ T cells and is present on various other immune cells under specific conditions. The receptor binds to the OX40L its ligand which is expressed on antigen-presenting cells such as dendritic cells and B cells.
- Biological function summary
CD134 influences T cell proliferation survival and memory formation being an important component of the immune system's adaptive response. OX40L engagement with CD134 enhances the activation and survival of T cells promoting long-term immunity. This interaction does not form part of a larger complex but functions as a direct signaling mechanism that augments T cell receptor (TCR) signaling leading to increased cytokine production and sustained immune responses essential for controlling infections and cancer.
- Pathways
CD134 signaling intersects with the nuclear factor-kappa B (NF-κB) and phosphoinositide 3-kinase (PI3K) pathways. These pathways play significant roles in regulating immune responses and cell survival. Through the NF-κB pathway CD134 influences gene transcription related to immune activation. It also connects with other immune regulatory proteins such as CD28 which further potentiates T cell responses and enhances effector functions.
- Associated diseases and disorders
CD134 has connections to autoimmune diseases and cancers. Overactivity of the CD134-OX40L pathway can lead to exacerbated autoimmune conditions such as lupus. This receptor has also been implicated in tumor immunity where its activation enhances the anti-tumor response of T cells. CD134 interacts with CTLA-4 another immune checkpoint protein playing a role in balancing immune activation and regulation within these disease contexts.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunoprecipitation - Anti-CD134 / OX40L receptor antibody [EPR23000-42] - BSA and Azide free (ab264470), expandable thumbnail](https://content.abcam.com/products/images/cd134-ox40l-receptor-antibody-epr23000-42-bsa-and-azide-free-ab264470--immunoprecipitation-img3592.jpg "Immunoprecipitation - Anti-CD134 / OX40L receptor antibody [EPR23000-42] - BSA and Azide free (ab264470)")
Immunoprecipitation - Anti-CD134 / OX40L receptor antibody [EPR23000-42] - BSA and Azide free (ab264470)
CD134 / OX40L receptor was immunoprecipitated from 0.35 mg Human thymus lysate with Anti-CD134 / OX40L receptor antibody [EPR23000-42] ab264466 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-CD134 / OX40L receptor antibody [EPR23000-42] ab264466 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/1000 dilution.
Lane 1: Human thymus lysate 10ug
Lane 2: Anti-CD134 / OX40L receptor antibody [EPR23000-42] ab264466 IP in Human thymus lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CD134 / OX40L receptor antibody [EPR23000-42] ab264466 in Human thymus lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 min.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD134 / OX40L receptor antibody [EPR23000-42] ab264466).
All lanes: Immunoprecipitation - Anti-CD134 / OX40L receptor antibody [EPR23000-42] (Anti-CD134 / OX40L receptor antibody [EPR23000-42] ab264466)
Predicted band size: 29 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [EPR23000-42] - BSA and Azide free (ab264470), expandable thumbnail](https://content.abcam.com/products/images/cd134-ox40l-receptor-antibody-epr23000-42-bsa-and-azide-free-ab264470--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104649.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [EPR23000-42] - BSA and Azide free (ab264470)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [EPR23000-42] - BSA and Azide free (ab264470)
Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymphoma tissue labeling CD134 / OX40L receptor with Anti-CD134 / OX40L receptor antibody [EPR23000-42] ab264466 at 1/4000 dilution (0.13 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on human Hodgkin's lymphoma (PMID/8547142) Counterstained with Hematoxylin.
Secondary antibody only control/ Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD134 / OX40L receptor antibody [EPR23000-42] ab264466).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [EPR23000-42] - BSA and Azide free (ab264470), expandable thumbnail](https://content.abcam.com/products/images/cd134-ox40l-receptor-antibody-epr23000-42-bsa-and-azide-free-ab264470--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img84042.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [EPR23000-42] - BSA and Azide free (ab264470)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [EPR23000-42] - BSA and Azide free (ab264470)
Immunohistochemical analysis of paraffin-embedded Human thymus tissue labeling CD134 / OX40L with Anti-CD134 / OX40L receptor antibody [EPR23000-42] ab264466 at 1/4000 dilution (0.13 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on T cells of human thymus (PMID/8547142) Counterstained with Hematoxylin.
Secondary antibody only control/ Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD134 / OX40L receptor antibody [EPR23000-42] ab264466).
![Flow Cytometry (Intracellular) - Anti-CD134 / OX40L receptor antibody [EPR23000-42] - BSA and Azide free (ab264470), expandable thumbnail](https://content.abcam.com/products/images/cd134-ox40l-receptor-antibody-epr23000-42-bsa-and-azide-free-ab264470--flow-cytometry-intracellular-img17655.jpg "Flow Cytometry (Intracellular) - Anti-CD134 / OX40L receptor antibody [EPR23000-42] - BSA and Azide free (ab264470)")
Flow Cytometry (Intracellular) - Anti-CD134 / OX40L receptor antibody [EPR23000-42] - BSA and Azide free (ab264470)
Intracellular flow cytometric analysis of 2% Paraformaldehyde fixed, 0.1% Tween-20 permeabilized Human peripheral blood mononuclear cell (PBMC) treated with 10 μg/ml phytohemagglutinin (PHA) for 2days labelling CD132 / OX40L receptor with Anti-CD134 / OX40L receptor antibody [EPR23000-42] ab264466 at 1/500 dilution 9Right) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) control (Left). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
Cells were surface stained with anti-CD3 conjugated to Alexa Fluor® 647. Then fixed with 2% PFA followed by intracellularly stained with rabbit IgG (Left) or Anti-CD134 / OX40L receptor antibody [EPR23000-42] ab264466 (Right). (PMID: 9108415, 17609274).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD134 / OX40L receptor antibody [EPR23000-42] ab264466).
![Immunocytochemistry/ Immunofluorescence - Anti-CD134 / OX40L receptor antibody [EPR23000-42] - BSA and Azide free (ab264470), expandable thumbnail](https://content.abcam.com/products/images/cd134-ox40l-receptor-antibody-epr23000-42-bsa-and-azide-free-ab264470--immunocytochemistry-immunofluorescence-img91873.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CD134 / OX40L receptor antibody [EPR23000-42] - BSA and Azide free (ab264470)")
Immunocytochemistry/ Immunofluorescence - Anti-CD134 / OX40L receptor antibody [EPR23000-42] - BSA and Azide free (ab264470)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized human PBMCs labelling CD34 / OX40L with Anti-CD134 / OX40L receptor antibody [EPR23000-42] ab264466 at 1/50 (10 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing positive staining in human PBMC treated with phytohaemagglutinin (PHA) (10 μg/ml for 2 days) (PMID: 9108415). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 ug/ml) dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD134 / OX40L receptor antibody [EPR23000-42] ab264466).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [EPR23000-42] - BSA and Azide free (ab264470), expandable thumbnail](https://content.abcam.com/products/images/cd134-ox40l-receptor-antibody-epr23000-42-bsa-and-azide-free-ab264470--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423103.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [EPR23000-42] - BSA and Azide free (ab264470)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [EPR23000-42] - BSA and Azide free (ab264470)
Tissue Microarrays stained for "Anti-CD134 / OX40L receptor antibody [EPR23000-42]" using "Anti-CD134 / OX40L receptor antibody [EPR23000-42] ab264466"in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). The sections were incubated with Anti-CD134 / OX40L receptor antibody [EPR23000-42] ab264466 at +4°C overnight followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [EPR23000-42] - BSA and Azide free (ab264470), expandable thumbnail](https://content.abcam.com/products/images/cd134-ox40l-receptor-antibody-epr23000-42-bsa-and-azide-free-ab264470--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423103.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [EPR23000-42] - BSA and Azide free (ab264470)")
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