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Mouse Recombinant Monoclonal CD134 / OX40L receptor antibody. Suitable for Flow Cyt, Protein Array, ICC/IF, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human TNFRSF4 aa 50-250.

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Images

Flow Cytometry - Anti-CD134 / OX40L receptor antibody [OX40/3108] (AB270727), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [OX40/3108] (AB270727), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CD134 / OX40L receptor antibody [OX40/3108] (AB270727), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [OX40/3108] (AB270727), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [OX40/3108] (AB270727), expandable thumbnail

Key facts

Isotype

IgG2c

Host species

Mouse

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human TNFRSF4 aa 50-250. The exact immunogen used to generate this antibody is proprietary information. Database link P43489

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytProtein ArrayICC/IFIHC-P
Human
Tested
Expected
Tested
Tested
Chimpanzee
Predicted
Predicted
Predicted
Predicted
Recombinant full length protein - Human
Not recommended
Tested
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info
1.00000-2.00000 µg for 106 Cells
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Chimpanzee

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Recombinant full length protein - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Recombinant full length protein - Human

Dilution info

-

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Chimpanzee

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info
1.00000-2.00000 µg/mL
Notes

-

Predicted
Predicted

Species

Chimpanzee

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Recombinant full length protein - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info
1.00000-2.00000 µg/mL
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Chimpanzee

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Recombinant full length protein - Human

Dilution info

-

Notes

-

Target data

Function

Receptor for TNFSF4/OX40L/GP34. Is a costimulatory molecule implicated in long-term T-cell immunity.(Microbial infection) Acts as a receptor for human herpesvirus 6B/HHV-6B.

Alternative names

Recommended products

Mouse Recombinant Monoclonal CD134 / OX40L receptor antibody. Suitable for Flow Cyt, Protein Array, ICC/IF, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human TNFRSF4 aa 50-250.

Key facts

Isotype

IgG2c

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Recombinant Fragment Protein within Human TNFRSF4 aa 50-250. The exact immunogen used to generate this antibody is proprietary information. Database link P43489
Clone number

OX40/3108

Purification technique

Affinity purification Protein A/G

Light chain type

kappa

Concentration
Loading...
Purification notes

Purified from Bioreactor Concentrate.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD134 also known as OX40 or TNFRSF4 is a receptor that plays an essential role in immune response regulation. This receptor is part of the tumor necrosis factor receptor (TNFR) superfamily and has a molecular mass of approximately 50 kDa. CD134 is expressed on activated T cells particularly CD4+ T cells and is present on various other immune cells under specific conditions. The receptor binds to the OX40L its ligand which is expressed on antigen-presenting cells such as dendritic cells and B cells.

Biological function summary

CD134 influences T cell proliferation survival and memory formation being an important component of the immune system's adaptive response. OX40L engagement with CD134 enhances the activation and survival of T cells promoting long-term immunity. This interaction does not form part of a larger complex but functions as a direct signaling mechanism that augments T cell receptor (TCR) signaling leading to increased cytokine production and sustained immune responses essential for controlling infections and cancer.

Pathways

CD134 signaling intersects with the nuclear factor-kappa B (NF-κB) and phosphoinositide 3-kinase (PI3K) pathways. These pathways play significant roles in regulating immune responses and cell survival. Through the NF-κB pathway CD134 influences gene transcription related to immune activation. It also connects with other immune regulatory proteins such as CD28 which further potentiates T cell responses and enhances effector functions.

Associated diseases and disorders

CD134 has connections to autoimmune diseases and cancers. Overactivity of the CD134-OX40L pathway can lead to exacerbated autoimmune conditions such as lupus. This receptor has also been implicated in tumor immunity where its activation enhances the anti-tumor response of T cells. CD134 interacts with CTLA-4 another immune checkpoint protein playing a role in balancing immune activation and regulation within these disease contexts.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Flow Cytometry - Anti-CD134 / OX40L receptor antibody [OX40/3108] (ab270727), expandable thumbnail

    Flow Cytometry - Anti-CD134 / OX40L receptor antibody [OX40/3108] (ab270727)

    Flow cytometry analysis of human MOLT4 cells labeling CD134/OX40L (Blue) using ab270727 at 2 μg/ml, followed by goat anti-mouse IgG conjugated to CF488. Isotype control (Red).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [OX40/3108] (ab270727), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [OX40/3108] (ab270727)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD134/OX40L using ab270727 at 2 μg/ml in immunohistochemical analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-CD134 / OX40L receptor antibody [OX40/3108] (ab270727), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CD134 / OX40L receptor antibody [OX40/3108] (ab270727)

    Immunofluorescence analysis of human MOLT4 cells labeling CD134/OX40L (Green) using ab270727 at 2 μg/ml, followed by goat anti-mouse IgG conjugated to CF488. Nuclei are stained with Reddot (Red).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [OX40/3108] (ab270727), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [OX40/3108] (ab270727)

    Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for CD134/OX40L using ab270727 at 2 μg/ml in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [OX40/3108] (ab270727), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [OX40/3108] (ab270727)

    Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for CD134/OX40L using ab270727 at 2 μg/ml in immunohistochemical analysis.

  • Protein Array - Anti-CD134 / OX40L receptor antibody [OX40/3108] (ab270727), expandable thumbnail

    Protein Array - Anti-CD134 / OX40L receptor antibody [OX40/3108] (ab270727)

    Analysis of Protein Array containing more than 19,000 full-length human proteins using ab270727.

    Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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