Mouse Monoclonal CD134 / OX40L receptor antibody. Carrier free. Suitable for Flow Cyt, IHC-Fr and reacts with Rat samples.
Images
![Flow Cytometry - Anti-CD134 / OX40L receptor antibody [OX40] - BSA and Azide free (AB244589), expandable thumbnail](https://content.abcam.com/products/images/cd134-ox40l-receptor-antibody-ox40-bsa-and-azide-free-ab244589--flow-cytometry-img94447.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CD134 / OX40L receptor antibody [OX40] - BSA and Azide free (AB244589)")
Key facts
- Isotype
IgG2b
- Host species
Mouse
- Storage buffer
Constituents: PBS
- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt | IHC-Fr | |
|---|---|---|
| Rat | Tested | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 5 µg/mL | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
1 products for Alternative Version
Target data
Function
Receptor for TNFSF4/OX40L/GP34. Is a costimulatory molecule implicated in long-term T-cell immunity.(Microbial infection) Acts as a receptor for human herpesvirus 6B/HHV-6B.
Alternative names
Tumor necrosis factor receptor superfamily member 4, ACT35 antigen, OX40L receptor, TAX transcriptionally-activated glycoprotein 1 receptor, TNFRSF4, TXGP1L
Recommended products
Mouse Monoclonal CD134 / OX40L receptor antibody. Carrier free. Suitable for Flow Cyt, IHC-Fr and reacts with Rat samples.
Alternative names
Tumor necrosis factor receptor superfamily member 4, ACT35 antigen, OX40L receptor, TAX transcriptionally-activated glycoprotein 1 receptor, TNFRSF4, TXGP1L
Key facts
- Isotype
IgG2b
- Host species
Mouse
- Storage buffer
Constituents: PBS
- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
OX40
- Purification technique
Affinity purification Protein G
- Light chain type
kappa
- Concentration
- Purification notes
Purified from TCS.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Do Not Freeze
Notes
ab244589 is the carrier-free version of Anti-CD134 / OX40L receptor antibody [OX40] ab238475.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD134 also known as OX40 or TNFRSF4 is a receptor that plays an essential role in immune response regulation. This receptor is part of the tumor necrosis factor receptor (TNFR) superfamily and has a molecular mass of approximately 50 kDa. CD134 is expressed on activated T cells particularly CD4+ T cells and is present on various other immune cells under specific conditions. The receptor binds to the OX40L its ligand which is expressed on antigen-presenting cells such as dendritic cells and B cells.
- Biological function summary
CD134 influences T cell proliferation survival and memory formation being an important component of the immune system's adaptive response. OX40L engagement with CD134 enhances the activation and survival of T cells promoting long-term immunity. This interaction does not form part of a larger complex but functions as a direct signaling mechanism that augments T cell receptor (TCR) signaling leading to increased cytokine production and sustained immune responses essential for controlling infections and cancer.
- Pathways
CD134 signaling intersects with the nuclear factor-kappa B (NF-κB) and phosphoinositide 3-kinase (PI3K) pathways. These pathways play significant roles in regulating immune responses and cell survival. Through the NF-κB pathway CD134 influences gene transcription related to immune activation. It also connects with other immune regulatory proteins such as CD28 which further potentiates T cell responses and enhances effector functions.
- Associated diseases and disorders
CD134 has connections to autoimmune diseases and cancers. Overactivity of the CD134-OX40L pathway can lead to exacerbated autoimmune conditions such as lupus. This receptor has also been implicated in tumor immunity where its activation enhances the anti-tumor response of T cells. CD134 interacts with CTLA-4 another immune checkpoint protein playing a role in balancing immune activation and regulation within these disease contexts.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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Full details and terms and conditions can be found here:
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1 product image
![Flow Cytometry - Anti-CD134 / OX40L receptor antibody [OX40] - BSA and Azide free (ab244589), expandable thumbnail](https://content.abcam.com/products/images/cd134-ox40l-receptor-antibody-ox40-bsa-and-azide-free-ab244589--flow-cytometry-img94447.jpg "Flow Cytometry - Anti-CD134 / OX40L receptor antibody [OX40] - BSA and Azide free (ab244589)")
Flow Cytometry - Anti-CD134 / OX40L receptor antibody [OX40] - BSA and Azide free (ab244589)
This data was developed using the same antibody clone in a different buffer formulation (Anti-CD134 / OX40L receptor antibody [OX40] ab238475).
Lewis rat splenocytes (top) or Lewis rat splenocytes treated with 5μg/ml ConA for 3 days (bottom) were stained with Anti-CD134 / OX40L receptor antibody [OX40] ab238475 (right) or mouse IgG2b kappa (Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control ab170192) isotype (left). Splenocytes were incubated for 30 min on ice in 1x PBS containing 10 % rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (Anti-CD134 / OX40L receptor antibody [OX40] ab238475) or mouse IgG2b kappa (Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control ab170192) isotype (1x 106 in 100μl at 0.2μg/ml) for 30 min on ice.The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor® 488, pre-adsorbed) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117) was used at 1/2000 dilution for 30 min on ice.
The cells were simultaneously stained with CD4.
Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on CD3 positive T cells.
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