AB244589

Anti-CD134 / OX40L receptor antibody [OX40] - BSA and Azide free

Name: Anti-CD134 / OX40L receptor antibody [OX40] - BSA and Azide free (ab244589) | Abcam
Brand: Abcam Limited
SKU: AB244589
Price: 905 USD
Availability: InStock

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Mouse Monoclonal CD134 / OX40L receptor antibody. Carrier free. Suitable for Flow Cyt, IHC-Fr and reacts with Rat samples.

View Alternative Names

CD134, TXGP1L, TNFRSF4, Tumor necrosis factor receptor superfamily member 4, ACT35 antigen, OX40L receptor, TAX transcriptionally-activated glycoprotein 1 receptor

1 Images

Flow Cytometry - Anti-CD134 / OX40L receptor antibody [OX40] - BSA and Azide free (AB244589)

Flow Cyt

Lab

Flow Cytometry - Anti-CD134 / OX40L receptor antibody [OX40] - BSA and Azide free (AB244589)

This data was developed using the same antibody clone in a different buffer formulation (ab238475).

Lewis rat splenocytes (top) or Lewis rat splenocytes treated with 5μg/ml ConA for 3 days (bottom) were stained with ab238475 (right) or mouse IgG2b kappa (ab170192) isotype (left). Splenocytes were incubated for 30 min on ice in 1x PBS containing 10 % rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab238475) or mouse IgG2b kappa (ab170192) isotype (1x 10⁶ in 100μl at 0.2μg/ml) for 30 min on ice.The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor^® 488, pre-adsorbed) (ab150117) was used at 1/2000 dilution for 30 min on ice.

The cells were simultaneously stained with CD4.

Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on CD3 positive T cells.

519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-CD134 / OX40L receptor antibody [OX40]
Unconjugated

Anti-CD134 / OX40L receptor antibody [OX40]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OX40

Isotype

IgG2b

Light chain type

kappa

Carrier free

Yes

Reacts with

Rat

Applications

Flow Cyt, IHC-Fr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "5 µg/mL", "IHCFr-species-notes": "<p></p>" } } }

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Product details

ab244589 is the carrier-free version of ab238475.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

Purified from TCS.

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD134 also known as OX40 or TNFRSF4 is a receptor that plays an essential role in immune response regulation. This receptor is part of the tumor necrosis factor receptor (TNFR) superfamily and has a molecular mass of approximately 50 kDa. CD134 is expressed on activated T cells particularly CD4+ T cells and is present on various other immune cells under specific conditions. The receptor binds to the OX40L its ligand which is expressed on antigen-presenting cells such as dendritic cells and B cells.

Biological function summary

CD134 influences T cell proliferation survival and memory formation being an important component of the immune system's adaptive response. OX40L engagement with CD134 enhances the activation and survival of T cells promoting long-term immunity. This interaction does not form part of a larger complex but functions as a direct signaling mechanism that augments T cell receptor (TCR) signaling leading to increased cytokine production and sustained immune responses essential for controlling infections and cancer.

Pathways

CD134 signaling intersects with the nuclear factor-kappa B (NF-κB) and phosphoinositide 3-kinase (PI3K) pathways. These pathways play significant roles in regulating immune responses and cell survival. Through the NF-κB pathway CD134 influences gene transcription related to immune activation. It also connects with other immune regulatory proteins such as CD28 which further potentiates T cell responses and enhances effector functions.

CD134 has connections to autoimmune diseases and cancers. Overactivity of the CD134-OX40L pathway can lead to exacerbated autoimmune conditions such as lupus. This receptor has also been implicated in tumor immunity where its activation enhances the anti-tumor response of T cells. CD134 interacts with CTLA-4 another immune checkpoint protein playing a role in balancing immune activation and regulation within these disease contexts.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for TNFSF4/OX40L/GP34. Is a costimulatory molecule implicated in long-term T-cell immunity.. (Microbial infection) Acts as a receptor for human herpesvirus 6B/HHV-6B.

See full target information TNFRSF4

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD134 / OX40L receptor antibody [OX40] - BSA and Azide free

Flow Cytometry - Anti-CD134 / OX40L receptor antibody [OX40] - BSA and Azide free (AB244589)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Product promise