AB245756

Anti-CD134 / OX40L receptor antibody [SP195] - BSA and Azide free

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Recombinant Monoclonal CD134 / OX40L receptor antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CD134, TXGP1L, TNFRSF4, Tumor necrosis factor receptor superfamily member 4, ACT35 antigen, OX40L receptor, TAX transcriptionally-activated glycoprotein 1 receptor

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [SP195] - BSA and Azide free (AB245756)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [SP195] - BSA and Azide free (AB245756)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling CD134 / OX40L receptor with ab228464 at 1/100 dilution (4.63 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228464)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [SP195] - BSA and Azide free (AB245756)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD134 / OX40 using ab228464 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS#44; BSA#44; and sodium azide (ab228464)

Unconjugated

Anti-CD134 / OX40L receptor antibody [SP195] - C-terminal
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-CD134 / OX40L receptor antibody [SP195] - C-terminal
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-CD134 / OX40L receptor antibody [SP195] - C-terminal
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-CD134 / OX40L receptor antibody [SP195] - C-terminal
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-CD134 / OX40L receptor antibody [SP195] - C-terminal
578 PE

PE Anti-CD134 / OX40L receptor antibody [SP195] - C-terminal
660 APC

APC Anti-CD134 / OX40L receptor antibody [SP195] - C-terminal
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-CD134 / OX40L receptor antibody [SP195] - C-terminal

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP195

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.Primary antibody incubation for 10 minutes at room temperature.</p>" } } }

style

left-column

Product details

ab245756 is the carrier-free version of ab228464.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

Purified from TCS by protein A/G.

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD134 also known as OX40 or TNFRSF4 is a receptor that plays an essential role in immune response regulation. This receptor is part of the tumor necrosis factor receptor (TNFR) superfamily and has a molecular mass of approximately 50 kDa. CD134 is expressed on activated T cells particularly CD4+ T cells and is present on various other immune cells under specific conditions. The receptor binds to the OX40L its ligand which is expressed on antigen-presenting cells such as dendritic cells and B cells.

Biological function summary

CD134 influences T cell proliferation survival and memory formation being an important component of the immune system's adaptive response. OX40L engagement with CD134 enhances the activation and survival of T cells promoting long-term immunity. This interaction does not form part of a larger complex but functions as a direct signaling mechanism that augments T cell receptor (TCR) signaling leading to increased cytokine production and sustained immune responses essential for controlling infections and cancer.

Pathways

CD134 signaling intersects with the nuclear factor-kappa B (NF-κB) and phosphoinositide 3-kinase (PI3K) pathways. These pathways play significant roles in regulating immune responses and cell survival. Through the NF-κB pathway CD134 influences gene transcription related to immune activation. It also connects with other immune regulatory proteins such as CD28 which further potentiates T cell responses and enhances effector functions.

CD134 has connections to autoimmune diseases and cancers. Overactivity of the CD134-OX40L pathway can lead to exacerbated autoimmune conditions such as lupus. This receptor has also been implicated in tumor immunity where its activation enhances the anti-tumor response of T cells. CD134 interacts with CTLA-4 another immune checkpoint protein playing a role in balancing immune activation and regulation within these disease contexts.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Receptor for TNFSF4/OX40L/GP34. Is a costimulatory molecule implicated in long-term T-cell immunity.. (Microbial infection) Acts as a receptor for human herpesvirus 6B/HHV-6B.

See full target information TNFRSF4

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

iScience 25:103785 PubMed35146396

2022

PD-L1CD8 T cells enrichment in lung cancer exerted regulatory function and tumor-promoting tolerance.

Applications

Unspecified application

Species

Unspecified reactive species

Yingxia Zheng,Li Han,Zheyi Chen,Yiyang Li,Bingqian Zhou,Rui Hu,Shiyu Chen,Haibo Xiao,Yanhui Ma,Guohua Xie,Junyao Yang,Xianting Ding,Lisong Shen

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD134 / OX40L receptor antibody [SP195] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [SP195] - BSA and Azide free (AB245756)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [SP195] - BSA and Azide free (AB245756)

Related conjugates and formulations (8)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise