AB252559

Anti-CD137 antibody [BLR051F] - BSA free

Recombinant
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(3 Publications)

Rabbit Recombinant Monoclonal CD137 antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TNFRSF9.

View Alternative Names

CD137, ILA, TNFRSF9, Tumor necrosis factor receptor superfamily member 9, 4-1BB ligand receptor, CDw137, T-cell antigen 4-1BB homolog, T-cell antigen ILA

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD137 antibody [BLR051F] - BSA free (AB252559)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD137 antibody [BLR051F] - BSA free (AB252559)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD37 with ab252559 at a 1/200 dilution in immunohistochemical analysis. DAB staining. HRP-conjugated goat anti-rabbit IgG secondary.

Immunocytochemistry/ Immunofluorescence - Anti-CD137 antibody [BLR051F] - BSA free (AB252559)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD137 antibody [BLR051F] - BSA free (AB252559)

FFPE section of HDLM2 (Human cell line) cells labeling CD37 using ab252559 at a 1/200 dilution in ICC.

Secondary : HRP-conjugated goat anti-rabbit IgG.

Substrate : DAB.

Supplier Data

Western blot - Anti-CD137 antibody [BLR051F] - BSA free (AB252559)

Prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-CD137 antibody [BLR051F] - BSA free (ab252559) at 1/1000 dilution

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

SUP-M2 (Human cell line) whole cell lysate at 50 µg

Lane 5:

HDML2 (Human cell line) whole cell lysate at 50 µg

Lane 6:

My-La-CD4+ (Human cell line) whole cell lysate at 50 µg

Lane 7:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

Predicted band size: 28 kDa

false

Exposure time: 3min

Supplier Data

Immunoprecipitation - Anti-CD137 antibody [BLR051F] - BSA free (AB252559)

CD37 was immunoprecipitated from HDLM2 (Human cell line) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab252559 used for IP at 6 μl per reaction. For WB 1/1000 dilution.

Lane 1 : ab252559 IP in HDLM2 whole cell lysate.
Lane 2 : Control IgG IP in HDLM2 whole cell lysate.
Chemiluminescence detection : 30 seconds.

All lanes:

Immunoprecipitation - Anti-CD137 antibody [BLR051F] - BSA free (ab252559)

Predicted band size: 28 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR051F

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, ICC/IF, IP

applications

Immunogen

Recombinant Fragment Protein within Human TNFRSF9. The exact immunogen used to generate this antibody is proprietary information.

Q07011

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "6 μl/1 mg lysate", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections." } } }

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Product details

This product is sold under License from Bethyl Laboratories, Inc.

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Properties and storage information

Form

Liquid

Purification notes

Purified from cell culture supernatant.

Storage buffer

pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 99% Borate buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD137 also known as 4-1BB or TNFRSF9 is a protein that functions as a costimulatory receptor in the immune system. It belongs to the tumor necrosis factor receptor (TNFR) superfamily. CD137 has a mass of approximately 30 kDa. Expression of CD137 occurs mainly on activated T lymphocytes including both CD8+ and CD4+ T cells as well as natural killer (NK) cells dendritic cells and monocytes. The CD137 ligand (CD137L) is the counterpart that engages this receptor transmitting signals important for immune response regulation.

Biological function summary

CD137 plays an important role in T cell activation and survival. It is a member of a receptor-ligand system that enhances immune cell functions. CD137 is part of a receptor complex that when bound by its ligand CD137L promotes cell proliferation and cytokine production. It also supports the development of memory T cells contributing to the body's ability to mount long-term immune responses.

Pathways

CD137 takes part in the NF-kB signaling and MAPK pathways. These pathways are vital for regulating immune responses and inflammation. CD137 signaling through these pathways involves interaction with proteins such as TRAF1 and TRAF2 which help mediate downstream effects critical for cell survival and proliferation. These interactions enhance the body's defense against pathogens and tumors.

CD137 is associated with cancer immunotherapy and autoimmune diseases. In cancer CD137 enhances the anti-tumor activity of immune cells making it a target for immunotherapy strategies. For autoimmune diseases abnormal CD137 expression can lead to excessive immune responses contributing to disease pathogenesis. The protein is also connected to TNF-alpha through its involvement in inflammation suggesting potential intervention points for modulating immune response in related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for TNFSF9/4-1BBL. Conveys a signal that enhances CD8(+) T-cell survival, cytotoxicity, and mitochondrial activity, thereby promoting immunity against viruses and tumors (Probable).

See full target information TNFRSF9

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

BMC biology 23:107 PubMed40275312

2025

Single-nucleus RNA sequencing defines adipose tissue subpopulations that contribute to Tibetan pig cold adaptation.

Applications

Unspecified application

Species

Unspecified reactive species

Jiali Liu,Yao Jiang,Tianxia Liu,Chuanhe Chen,Linya Chui,Along Cui,Xueping Zhang,Xiao Wang,Yu Wang,Chunhuai Yang,Ying Zhang,Tianwen Wu,Shulin Yang,Jiaojiao Huang,Cong Tao,Jianguo Zhao,Yanfang Wang

Immunity, inflammation and disease 12:e70005 PubMed39267471

2024

B7-H3 promotes nasopharyngeal carcinoma progression by regulating CD8+ T cell exhaustion.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaoen Ma,Gui Chen,Hao Li,Saixuan Yang,Yali Xu,Bolin Pan,Wuping Lai,Guangui Chen,Wenjing Liao,Xiaowen Zhang

Journal of cachexia, sarcopenia and muscle 14:1003-1018 PubMed36864250

2023

Nicotinamide Phosphoribosyltransferase-elevated NAD biosynthesis prevents muscle disuse atrophy by reversing mitochondrial dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Yao Zhang,Yingming Wang,Shuai Lu,Rui Zhong,Zhilin Liu,Qichun Zhao,Chongyang Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD137 antibody [BLR051F] - BSA free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD137 antibody [BLR051F] - BSA free (AB252559)

Immunocytochemistry/ Immunofluorescence - Anti-CD137 antibody [BLR051F] - BSA free (AB252559)

Western blot - Anti-CD137 antibody [BLR051F] - BSA free (AB252559)

Immunoprecipitation - Anti-CD137 antibody [BLR051F] - BSA free (AB252559)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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