AB209256

Anti-CD137 antibody [EPR20238]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal CD137 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CD137, ILA, TNFRSF9, Tumor necrosis factor receptor superfamily member 9, 4-1BB ligand receptor, CDw137, T-cell antigen 4-1BB homolog, T-cell antigen ILA

4 Images

Supplier Data

Western blot - Anti-CD137 antibody [EPR20238] (AB209256)

Blocking/Dilution buffer : 5% NFDM/TBST.

The MW observed is consistent with the literature : PMID 10528184; PMID 17560335.

All lanes:

Western blot - Anti-CD137 antibody [EPR20238] (ab209256) at 1/1000 dilution

Lane 1:

Human lymphoma lysate at 20 µg

Lane 2:

Human fetal spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 28 kDa

Observed band size: 36 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-CD137 antibody [EPR20238] (AB209256)

Blocking/Dilution buffer : 5% NFDM/TBST.

The MW observed is consistent with the literature : PMID 10528184; PMID 17560335.

All lanes:

Western blot - Anti-CD137 antibody [EPR20238] (ab209256) at 1/1000 dilution

All lanes:

H9 (Human cutaneous T Lymphocyte cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 28 kDa

Observed band size: 36 kDa,40 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-CD137 antibody [EPR20238] (AB209256)

Blocking/Dilution buffer : 5% NFDM/TBST.

The expression level of CD137 can be induced by TPA and Ionomycin [PMID 14648705].

The MW observed is consistent with the literature : PMID 10528184; PMID 17560335.

All lanes:

Western blot - Anti-CD137 antibody [EPR20238] (ab209256) at 1/1000 dilution

Lane 1:

Whole cell lysate from untreated Jurkat (Human T cell leukemia cell line from peripheral blood) at 10 µg

Lane 2:

Whole cell lysate from Jurkat cells treated with 10ng/ml Phorbol-12-myristate-13-acetate (TPA) and 1μM Ionomycin (<a href='/en-us/products/biochemicals/ionomycin-ca2-salt-ca2-ionophore-ab120116'>ab120116</a>) for 48 hours at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 28 kDa

Observed band size: 36 kDa,40 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-CD137 antibody [EPR20238] (AB209256)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CD137 antibody [EPR20238] (ab209256) at 1/5000 dilution

All lanes:

His-tagged CD137 Fc chimera recombinant protein containing human CD137 (aa24-186) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 28 kDa

Observed band size: 65 kDa

false

Exposure time: 4s

Carrier free

Anti-CD137 antibody [EPR20238] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20238

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD137 also known as 4-1BB or TNFRSF9 is a protein that functions as a costimulatory receptor in the immune system. It belongs to the tumor necrosis factor receptor (TNFR) superfamily. CD137 has a mass of approximately 30 kDa. Expression of CD137 occurs mainly on activated T lymphocytes including both CD8+ and CD4+ T cells as well as natural killer (NK) cells dendritic cells and monocytes. The CD137 ligand (CD137L) is the counterpart that engages this receptor transmitting signals important for immune response regulation.

Biological function summary

CD137 plays an important role in T cell activation and survival. It is a member of a receptor-ligand system that enhances immune cell functions. CD137 is part of a receptor complex that when bound by its ligand CD137L promotes cell proliferation and cytokine production. It also supports the development of memory T cells contributing to the body's ability to mount long-term immune responses.

Pathways

CD137 takes part in the NF-kB signaling and MAPK pathways. These pathways are vital for regulating immune responses and inflammation. CD137 signaling through these pathways involves interaction with proteins such as TRAF1 and TRAF2 which help mediate downstream effects critical for cell survival and proliferation. These interactions enhance the body's defense against pathogens and tumors.

CD137 is associated with cancer immunotherapy and autoimmune diseases. In cancer CD137 enhances the anti-tumor activity of immune cells making it a target for immunotherapy strategies. For autoimmune diseases abnormal CD137 expression can lead to excessive immune responses contributing to disease pathogenesis. The protein is also connected to TNF-alpha through its involvement in inflammation suggesting potential intervention points for modulating immune response in related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for TNFSF9/4-1BBL. Conveys a signal that enhances CD8(+) T-cell survival, cytotoxicity, and mitochondrial activity, thereby promoting immunity against viruses and tumors (Probable).

See full target information TNFRSF9

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Hepatology communications 9: PubMed40366121

2025

Single-cell transcription reveals hepatocyte-to-cholangiocyte reprogramming and biliary gene profile in biliary atresia.

Applications

Unspecified application

Species

Unspecified reactive species

Lingdu Meng,Min Du,Haodong Li,Fanyang Kong,Jiajian Yang,Rui Dong,Shan Zheng,Gong Chen,Zhen Shen,Junfeng Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD137 antibody [EPR20238]

Western blot - Anti-CD137 antibody [EPR20238] (AB209256)

Western blot - Anti-CD137 antibody [EPR20238] (AB209256)

Western blot - Anti-CD137 antibody [EPR20238] (AB209256)

Western blot - Anti-CD137 antibody [EPR20238] (AB209256)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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