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Rabbit Recombinant Monoclonal CD137 antibody. Suitable for IP, Flow Cyt, WB, ICC/IF and reacts with Mouse, Transfected cell line - Mouse samples. Cited in 1 publication.

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Images

Flow Cytometry - Anti-CD137 antibody [EPR23218-111] (AB243648), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CD137 antibody [EPR23218-111] (AB243648), expandable thumbnail
  • Western blot - Anti-CD137 antibody [EPR23218-111] (AB243648), expandable thumbnail
  • Immunoprecipitation - Anti-CD137 antibody [EPR23218-111] (AB243648), expandable thumbnail
  • Western blot - Anti-CD137 antibody [EPR23218-111] (AB243648), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBIHC-PICC/IF
Human
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Tested
Tested
Not recommended
Tested
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Recombinant full length protein - Human
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Transfected cell line - Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Tested

Tested
Tested

Species

Mouse

Dilution info

1/30

Notes

-

Not recommended
Not recommended

Species

Recombinant full length protein - Human, Rat, Human, Transfected cell line - Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/500

Notes

-

Not recommended
Not recommended

Species

Recombinant full length protein - Human, Rat, Human, Transfected cell line - Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Recombinant full length protein - Human, Rat, Human, Transfected cell line - Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Human, Rat, Recombinant full length protein - Human, Transfected cell line - Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/100

Notes

-

Species

Transfected cell line - Mouse

Dilution info

1/100

Notes

-

Not recommended
Not recommended

Species

Recombinant full length protein - Human, Rat, Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

Target data

Function

Receptor for TNFSF9/4-1BBL. Conveys a signal that enhances CD8(+) T-cell survival, cytotoxicity, and mitochondrial activity, thereby promoting immunity against viruses and tumors (By similarity).

Alternative names

Cd137, Ila, Ly63, Cd137, Ila, Ly63, Tnfrsf9, Tumor necrosis factor receptor superfamily member 9, 4-1BB ligand receptor, T-cell antigen 4-1BB

Recommended products

Rabbit Recombinant Monoclonal CD137 antibody. Suitable for IP, Flow Cyt, WB, ICC/IF and reacts with Mouse, Transfected cell line - Mouse samples. Cited in 1 publication.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR23218-111

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Flow Cytometry - Anti-CD137 antibody [EPR23218-111] (ab243648), expandable thumbnail

    Flow Cytometry - Anti-CD137 antibody [EPR23218-111] (ab243648)

    Flow cytometric analysis of Mouse splenocytes (treated with 2.5ug/ml concanavalin A for 72h) cells labelling CD137 with ab243648 at 1/500 (Right) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) isotype control (Left).

    Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

    Cells were stained with rabbit IgG (Left) or ab243648 (Right), then stained with anti-CD25 conjugated to BV421.

    Gated on viable cells.

  • Immunocytochemistry/ Immunofluorescence - Anti-CD137 antibody [EPR23218-111] (ab243648), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CD137 antibody [EPR23218-111] (ab243648)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T cells labelling CD137 with ab243648 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution (Red). Confocal image showing cytoplasmic and membranous staining in HEK-293T cells transfected with a GFP-tagged CD137 expression vector - the GPF tag is shown in Green. The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/1000 dilution.

  • Western blot - Anti-CD137 antibody [EPR23218-111] (ab243648), expandable thumbnail

    Western blot - Anti-CD137 antibody [EPR23218-111] (ab243648)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    CD137 is a type I transmembrane glycoprotein and has both monomeric and dimeric forms on the surface of activated T cells (PMID: 30355497).

    Exposure time: 3 minutes.

    All lanes: Western blot - Anti-CD137 antibody [EPR23218-111] (ab243648) at 1/1000 dilution

    Lane 1: Untreated mouse splenocytes, whole cell lysate at 20 µg

    Lane 2: Mouse splenocytes (treated with 2.5 μg/ml Concanavalin A for 72 hours), whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 28 kDa

    Observed band size: 21-24 kDa, 36-40 kDa

  • Immunoprecipitation - Anti-CD137 antibody [EPR23218-111] (ab243648), expandable thumbnail

    Immunoprecipitation - Anti-CD137 antibody [EPR23218-111] (ab243648)

    CD137 was immunoprecipitated from 0.35 mg mouse splenocyte (treated with 2.5 μg/ml Concanavalin A for 72 hours) whole cell lysate with ab243648 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab243648 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse splenocyte (treated with 2.5 μg/ml Concanavalin A for 72 hours) whole cell lysate 10 μg

    Lane 2: ab243648 IP in Mouse splenocyte (treated as above) whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab243648 in Mouse splenocyte (treated as above) whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 76 seconds.

    CD137 is a type I transmembrane glycoprotein and has both monomeric and dimeric forms on the surface of activated T cells (PMID: 30355497).

    All lanes: Immunoprecipitation - Anti-CD137 antibody [EPR23218-111] (ab243648)

    Predicted band size: 28 kDa, 63 kDa

    Observed band size: 21-24 kDa, 36-40 kDa

  • Western blot - Anti-CD137 antibody [EPR23218-111] (ab243648), expandable thumbnail

    Western blot - Anti-CD137 antibody [EPR23218-111] (ab243648)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 15 seconds.

    All lanes: Western blot - Anti-CD137 antibody [EPR23218-111] (ab243648) at 1/1000 dilution

    Lane 1: His-tagged mouse CD137 recombinant protein(aa24-187), 10 ng

    Lane 2: His-tagged human CD137 recombinant protein(aa24-186), 10 ng

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 28 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-CD137 antibody [EPR23218-111] (ab243648), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CD137 antibody [EPR23218-111] (ab243648)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse splenocytes cells labelling CD137 with ab243648 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic and membranous staining in mouse splenocytes treated with Concanavalin A (2.5 ug/ml) for 72 h. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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