Rabbit Recombinant Monoclonal CD137 antibody. Carrier free. Suitable for IP, Flow Cyt, WB, ICC/IF and reacts with Mouse, Recombinant fragment - Mouse, Transfected cell line - Mouse samples.
Images
![Flow Cytometry - Anti-CD137 antibody [EPR23218-111] - BSA and Azide free (AB270527), expandable thumbnail](https://content.abcam.com/products/images/cd137-antibody-epr23218-111-bsa-and-azide-free-ab270527--flow-cytometry-img17031.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CD137 antibody [EPR23218-111] - BSA and Azide free (AB270527)")
![Immunocytochemistry/ Immunofluorescence - Anti-CD137 antibody [EPR23218-111] - BSA and Azide free (AB270527), expandable thumbnail](https://content.abcam.com/products/images/cd137-antibody-epr23218-111-bsa-and-azide-free-ab270527--immunocytochemistry-immunofluorescence-img97683.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CD137 antibody [EPR23218-111] - BSA and Azide free (AB270527)")
![Immunoprecipitation - Anti-CD137 antibody [EPR23218-111] - BSA and Azide free (AB270527), expandable thumbnail](https://content.abcam.com/products/images/cd137-antibody-epr23218-111-bsa-and-azide-free-ab270527--immunoprecipitation-img12029.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-CD137 antibody [EPR23218-111] - BSA and Azide free (AB270527)")
![Immunocytochemistry/ Immunofluorescence - Anti-CD137 antibody [EPR23218-111] - BSA and Azide free (AB270527), expandable thumbnail](https://content.abcam.com/products/images/cd137-antibody-epr23218-111-bsa-and-azide-free-ab270527--immunocytochemistry-immunofluorescence-img91774.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CD137 antibody [EPR23218-111] - BSA and Azide free (AB270527)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Tested | Tested | Tested | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Recombinant fragment - Mouse | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
| Transfected cell line - Mouse | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Mouse, Human, Rat, Transfected cell line - Mouse | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Mouse, Human, Rat, Transfected cell line - Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Recombinant fragment - Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Transfected cell line - Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Transfected cell line - Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Mouse, Human, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant fragment - Mouse, Transfected cell line - Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
1 product for Alternative Version
Target data
Function
Receptor for TNFSF9/4-1BBL. Conveys a signal that enhances CD8(+) T-cell survival, cytotoxicity, and mitochondrial activity, thereby promoting immunity against viruses and tumors (By similarity).
Alternative names
CD137, Cd137, Ila, Ly63, Tnfrsf9, Tumor necrosis factor receptor superfamily member 9, 4-1BB ligand receptor, T-cell antigen 4-1BB
Recommended products
Rabbit Recombinant Monoclonal CD137 antibody. Carrier free. Suitable for IP, Flow Cyt, WB, ICC/IF and reacts with Mouse, Recombinant fragment - Mouse, Transfected cell line - Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR23218-111
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab270527 is the carrier-free version of Anti-CD137 antibody [EPR23218-111] ab243648.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD137 also known as 4-1BB or TNFRSF9 is a protein that functions as a costimulatory receptor in the immune system. It belongs to the tumor necrosis factor receptor (TNFR) superfamily. CD137 has a mass of approximately 30 kDa. Expression of CD137 occurs mainly on activated T lymphocytes including both CD8+ and CD4+ T cells as well as natural killer (NK) cells dendritic cells and monocytes. The CD137 ligand (CD137L) is the counterpart that engages this receptor transmitting signals important for immune response regulation.
- Biological function summary
CD137 plays an important role in T cell activation and survival. It is a member of a receptor-ligand system that enhances immune cell functions. CD137 is part of a receptor complex that when bound by its ligand CD137L promotes cell proliferation and cytokine production. It also supports the development of memory T cells contributing to the body's ability to mount long-term immune responses.
- Pathways
CD137 takes part in the NF-kB signaling and MAPK pathways. These pathways are vital for regulating immune responses and inflammation. CD137 signaling through these pathways involves interaction with proteins such as TRAF1 and TRAF2 which help mediate downstream effects critical for cell survival and proliferation. These interactions enhance the body's defense against pathogens and tumors.
- Associated diseases and disorders
CD137 is associated with cancer immunotherapy and autoimmune diseases. In cancer CD137 enhances the anti-tumor activity of immune cells making it a target for immunotherapy strategies. For autoimmune diseases abnormal CD137 expression can lead to excessive immune responses contributing to disease pathogenesis. The protein is also connected to TNF-alpha through its involvement in inflammation suggesting potential intervention points for modulating immune response in related diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Flow Cytometry - Anti-CD137 antibody [EPR23218-111] - BSA and Azide free (ab270527), expandable thumbnail](https://content.abcam.com/products/images/cd137-antibody-epr23218-111-bsa-and-azide-free-ab270527--flow-cytometry-img17031.jpg "Flow Cytometry - Anti-CD137 antibody [EPR23218-111] - BSA and Azide free (ab270527)")
Flow Cytometry - Anti-CD137 antibody [EPR23218-111] - BSA and Azide free (ab270527)
Flow cytometric analysis of Mouse splenocytes (treated with 2.5ug/ml concanavalin A for 72h) cells labelling CD137 with Anti-CD137 antibody [EPR23218-111] ab243648 at 1/500 (Right) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) isotype control (Left).
Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
Cells were stained with rabbit IgG (Left) or Anti-CD137 antibody [EPR23218-111] ab243648 (Right), then stained with anti-CD25 conjugated to BV421.
Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD137 antibody [EPR23218-111] ab243648).
![Immunocytochemistry/ Immunofluorescence - Anti-CD137 antibody [EPR23218-111] - BSA and Azide free (ab270527), expandable thumbnail](https://content.abcam.com/products/images/cd137-antibody-epr23218-111-bsa-and-azide-free-ab270527--immunocytochemistry-immunofluorescence-img97683.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CD137 antibody [EPR23218-111] - BSA and Azide free (ab270527)")
Immunocytochemistry/ Immunofluorescence - Anti-CD137 antibody [EPR23218-111] - BSA and Azide free (ab270527)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T cells labelling CD137 with Anti-CD137 antibody [EPR23218-111] ab243648 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution (Red). Confocal image showing cytoplasmic and membranous staining in HEK-293T cells transfected with a GFP-tagged CD137 expression vector - the GPF tag is shown in Green. The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD137 antibody [EPR23218-111] ab243648).
![Immunoprecipitation - Anti-CD137 antibody [EPR23218-111] - BSA and Azide free (ab270527), expandable thumbnail](https://content.abcam.com/products/images/cd137-antibody-epr23218-111-bsa-and-azide-free-ab270527--immunoprecipitation-img12029.jpg "Immunoprecipitation - Anti-CD137 antibody [EPR23218-111] - BSA and Azide free (ab270527)")
Immunoprecipitation - Anti-CD137 antibody [EPR23218-111] - BSA and Azide free (ab270527)
CD137 was immunoprecipitated from 0.35 mg mouse splenocyte (treated with 2.5 μg/ml Concanavalin A for 72 hours) whole cell lysate with Anti-CD137 antibody [EPR23218-111] ab243648 at 1/30 dilution. Western blot was performed on the immunoprecipitate using Anti-CD137 antibody [EPR23218-111] ab243648 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Mouse splenocyte (treated with 2.5 μg/ml Concanavalin A for 72 hours) whole cell lysate 10 μg
Lane 2: Anti-CD137 antibody [EPR23218-111] ab243648 IP in Mouse splenocyte (treated as above) whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CD137 antibody [EPR23218-111] ab243648 in Mouse splenocyte (treated as above) whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 76 seconds.
CD137 is a type I transmembrane glycoprotein and has both monomeric and dimeric forms on the surface of activated T cells (PMID: 30355497).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD137 antibody [EPR23218-111] ab243648).
All lanes: Immunoprecipitation - Anti-CD137 antibody [EPR23218-111] (Anti-CD137 antibody [EPR23218-111] ab243648)
Predicted band size: 28 kDa, 63 kDa
Observed band size: 21-24 kDa, 36-40 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-CD137 antibody [EPR23218-111] - BSA and Azide free (ab270527), expandable thumbnail](https://content.abcam.com/products/images/cd137-antibody-epr23218-111-bsa-and-azide-free-ab270527--immunocytochemistry-immunofluorescence-img91774.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CD137 antibody [EPR23218-111] - BSA and Azide free (ab270527)")
Immunocytochemistry/ Immunofluorescence - Anti-CD137 antibody [EPR23218-111] - BSA and Azide free (ab270527)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse splenocytes cells labelling CD137 with Anti-CD137 antibody [EPR23218-111] ab243648 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic and membranous staining in mouse splenocytes treated with Concanavalin A (2.5 ug/ml) for 72 h. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD137 antibody [EPR23218-111] ab243648).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com