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AB181470

Anti-CD14 antibody [1H5D8]

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(25 Publications)

Anti-CD14 antibody [1H5D8] (ab181470) is a mouse monoclonal antibody detecting CD14 in Western Blot, IHC-P, ICC/IF. Suitable for Human.

- Over 10 publications

View Alternative Names

CD14, Monocyte differentiation antigen CD14, My23 antigen, Myeloid cell-specific leucine-rich glycoprotein

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [1H5D8] (AB181470)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [1H5D8] (AB181470)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling CD14 with ab181470 at 1/200 dilution with DAB staining.

Immunocytochemistry/ Immunofluorescence - Anti-CD14 antibody [1H5D8] (AB181470)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD14 antibody [1H5D8] (AB181470)

Immunofluorescent analysis of HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling CD14 with ab181470 at 1/200 dilution (green). Blue : DRAQ5 fluorescent DNA dye.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [1H5D8] (AB181470)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [1H5D8] (AB181470)

Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue labeling CD14 with ab181470 at 1/200 dilution with DAB staining.

Western blot - Anti-CD14 antibody [1H5D8] (AB181470)
  • WB

Supplier Data

Western blot - Anti-CD14 antibody [1H5D8] (AB181470)

Expected MWt is 46.8 kDa.

All lanes:

Western blot - Anti-CD14 antibody [1H5D8] (ab181470) at 1/500 dilution

All lanes:

Human CD14 (aa 20-214) recombinant protein

Predicted band size: 40 kDa

false

Western blot - Anti-CD14 antibody [1H5D8] (AB181470)
  • WB

Supplier Data

Western blot - Anti-CD14 antibody [1H5D8] (AB181470)

All lanes:

Western blot - Anti-CD14 antibody [1H5D8] (ab181470) at 1/500 dilution

Lane 1:

Non-transfected HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate

Lane 2:

CD14 (aa 20-214)-hIgGFc transfected HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate

Predicted band size: 40 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

1H5D8

Isotype

IgG2a

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CD14 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

P08571

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Product details

What is this antibody validated in?
Anti-CD14 antibody [1H5D8] (ab181470) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

What is the molecular weight of CD14?
Anti-CD14 [1H5D8] (ab181470) specifically detects a band for CD14 (UniProt: P08571) at a molecular weight of 40kDa.

Trusted by the scientific community
Anti-CD14 [1H5D8] (ab181470) was first used in a scientific publication in 2013 and has been cited over 10 times in peer-reviewed journals.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified from tissue culture supernatant.
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD14 also known as cluster of differentiation 14 is a glycoprotein with a molecular mass of approximately 55 kDa. This protein is expressed mainly on the surface of macrophages monocytes and to a lesser extent on neutrophils. CD14 exists in two forms: membrane-bound (mCD14) and soluble (sCD14). It functions as a co-receptor along with TLR4 (Toll-like receptor 4) and MD-2 for the detection of bacterial lipopolysaccharide (LPS) which is an important component of the outer membrane of Gram-negative bacteria.
Biological function summary

This glycoprotein plays a significant role in the innate immune system by recognizing pathogen-associated molecular patterns (PAMPs). It is part of a receptor complex where CD14 acts alongside LPS binding protein (LPS-R) TLR4 and MD-2 to facilitate pro-inflammatory signaling in response to microbial invasion. By binding to LPS CD14 initiates and amplifies the immune response leading to the production of cytokines and the recruitment of other immune cells to the site of infection.

Pathways

CD14 interacts with the toll-like receptor signaling pathway notably involving TLR4. Upon LPS detection CD14 assists in the activation of TLR4 triggering downstream signaling cascades such as NF-κB and MAPK pathways. These pathways result in the transcription of inflammatory cytokines and are essential for the host defense mechanism. The protein complex involving CD14 further interacts with adaptor proteins like MyD88 and TRIF facilitating signal propagation and the immune system's ability to respond to pathogens.

Researchers have linked CD14 to sepsis and atherosclerosis. High levels of soluble CD14 (sCD14) are often observed in patients with sepsis indicating its participation in excessive systemic inflammation. In atherosclerosis CD14's role in recognizing LPS contributes to chronic inflammation and the development of plaques in arteries. Through these diseases CD14 connects with TLR4 emphasizing its impact on inflammatory responses and its potential as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Coreceptor for bacterial lipopolysaccharide (PubMed : 1698311, PubMed : 23264655). In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed : 20133493, PubMed : 22265692, PubMed : 23264655). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed : 8612135). Acts as a coreceptor for TLR2 : TLR6 heterodimer in response to diacylated lipopeptides and for TLR2 : TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (PubMed : 16880211). Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-) (PubMed : 23880187).
See full target information CD14
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Publications (25)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2406282 PubMed39665236

2024

Platelet-Monocyte Aggregate Instigates Inflammation and Vasculopathy in Kawasaki Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Zhang,Cuiping Jia,Manli Guo,Qian Chen,Ying Wen,Ting Wang,Yinyin Xie,Xuejiao Fan,Jingwen Gao,Timur O Yarovinsky,Renjing Liu,Zhiyong Jiang,Mengmeng Wang,Jin Zhou,Di Che,Lanyan Fu,Richard Edelson,Xiaoqiong Gu,John Hwa,Wai Ho Tang

Nature medicine 30:3150-3162 PubMed39095597

2024

Safety and reactogenicity of a controlled human infection model of sand fly-transmitted cutaneous leishmaniasis.

Applications

Unspecified application

Species

Unspecified reactive species

Vivak Parkash,Helen Ashwin,Shoumit Dey,Jovana Sadlova,Barbora Vojtkova,Katrien Van Bocxlaer,Rebecca Wiggins,David Thompson,Nidhi Sharma Dey,Charles L Jaffe,Eli Schwartz,Petr Volf,Charles J N Lacey,Alison M Layton,Paul M Kaye

Journal of cancer research and clinical oncology 150:341 PubMed38976030

2024

Effects of the subtypes of apolipoprotein E on immune inhibition and prognosis in patients with Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Bowen Gao,Peiyun Zhou,Li Wang,Zhutao Wang,Yong Yi,Xian Li,Jian Zhou,Jia Fan,Shuangjian Qiu,Yang Xu

iScience 27:109213 PubMed38439953

2024

CD169 classical monocyte as an important participant in Graves' ophthalmopathy through CXCL12-CXCR4 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Dongliang Wang,Jie Ling,RongQiang Tan,Huishi Wang,Yixin Qu,Xingyi Li,Jinshan Lin,Qikai Zhang,Qiuling Hu,Zhong Liu,Zhaojing Lu,Yuheng Lin,Li Sun,Dingqiao Wang,Ming Zhou,Zhuoxing Shi,Wuyou Gao,Huijing Ye,Xianchai Lin

Annals of the rheumatic diseases 83:926-944 PubMed38325908

2024

Unveiling inflammatory and prehypertrophic cell populations as key contributors to knee cartilage degeneration in osteoarthritis using multi-omics data integration.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Fan,Xuzhao Bian,Xiaogao Meng,Lei Li,Laiyi Fu,Yanan Zhang,Long Wang,Yan Zhang,Dalong Gao,Xiong Guo,Mikko Juhani Lammi,Guangdun Peng,Shiquan Sun

Nature biomedical engineering 8:345-360 PubMed38114742

2023

Analysis of off-tumour toxicities of T-cell-engaging bispecific antibodies via donor-matched intestinal organoids and tumouroids.

Applications

Unspecified application

Species

Unspecified reactive species

Marius F Harter,Timothy Recaldin,Regine Gerard,Blandine Avignon,Yannik Bollen,Cinzia Esposito,Karolina Guja-Jarosz,Kristina Kromer,Adrian Filip,Julien Aubert,Anneliese Schneider,Marina Bacac,Michael Bscheider,Nadine Stokar-Regenscheit,Salvatore Piscuoglio,Joep Beumer,Nikolche Gjorevski

Scientific reports 13:15170 PubMed37704707

2023

Osteoclast differentiation and dynamic mRNA expression during mice embryonic palatal bone development.

Applications

Unspecified application

Species

Unspecified reactive species

Yongzhen Lai,Yan Guo,Caiyu Liao,Chuanqing Mao,Jing Liu,Chengyan Ren,Wen Yang,Lin Luo,Weihui Chen

Journal of translational medicine 21:366 PubMed37280612

2023

Atrial epicardial adipose tissue abundantly secretes myeloperoxidase and activates atrial fibroblasts in patients with atrial fibrillation.

Applications

Unspecified application

Species

Unspecified reactive species

Eva R Meulendijks,Rushd F M Al-Shama,Makiri Kawasaki,Benedetta Fabrizi,Jolien Neefs,Robin Wesselink,Auriane C Ernault,Sander Piersma,Thang V Pham,Connie R Jimenez,Jaco C Knol,Wim J P van Boven,Antoine H G Driessen,Tim A C de Vries,Britt van der Leeden,Hans W M Niessen,Onno J de Boer,Sébastien P J Krul,Joris R de Groot

International journal of molecular sciences 24: PubMed37298206

2023

Human Monocytes Are Suitable Carriers for the Delivery of Oncolytic Herpes Simplex Virus Type 1 In Vitro and in a Chicken Embryo Chorioallantoic Membrane Model of Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Alberto Reale,Lea Krutzke,Massimiliano Cadamuro,Adriana Vitiello,Jens von Einem,Stefan Kochanek,Giorgio Palù,Cristina Parolin,Arianna Calistri

Biochimica et biophysica acta. Molecular basis of disease 1869:166707 PubMed37001702

2023

Single cell RNA-seq resolution revealed CCR1/SELL/XAF CD14 monocytes mediated vascular endothelial cell injuries in Kawasaki disease and COVID-19.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoliang Liu,Tingting Luo,Zhenxin Fan,Jiawen Li,Yue Zhang,Guoyan Lu,Mingyi Lv,Sha Lin,Ziwen Cai,Jinbao Zhang,Kaiyu Zhou,Junling Guo,Yimin Hua,Yaoyao Zhang,Yifei Li
View all publications
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