AB106285

Anti-CD14 antibody

Name: Anti-CD14 antibody (ab106285) | Abcam
Brand: Abcam Limited
SKU: AB106285
Price: 535 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(6 Publications)

Rabbit Polyclonal CD14 antibody. Suitable for WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CD14 aa 1-300.

View Alternative Names

CD14, Monocyte differentiation antigen CD14, My23 antigen, Myeloid cell-specific leucine-rich glycoprotein

1 Images

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Western blot - Anti-CD14 antibody (AB106285)

All lanes:

Western blot - Anti-CD14 antibody (ab106285) at 1/1000 dilution

Lane 1:

Human fetal thymus lysate

Lane 2:

Hep2 cell lysate

Predicted band size: 40 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Recombinant Fragment Protein within Human CD14 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

P08571

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.02% Sodium azide Constituents: 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD14 also known as cluster of differentiation 14 is a glycoprotein with a molecular mass of approximately 55 kDa. This protein is expressed mainly on the surface of macrophages monocytes and to a lesser extent on neutrophils. CD14 exists in two forms: membrane-bound (mCD14) and soluble (sCD14). It functions as a co-receptor along with TLR4 (Toll-like receptor 4) and MD-2 for the detection of bacterial lipopolysaccharide (LPS) which is an important component of the outer membrane of Gram-negative bacteria.

Biological function summary

This glycoprotein plays a significant role in the innate immune system by recognizing pathogen-associated molecular patterns (PAMPs). It is part of a receptor complex where CD14 acts alongside LPS binding protein (LPS-R) TLR4 and MD-2 to facilitate pro-inflammatory signaling in response to microbial invasion. By binding to LPS CD14 initiates and amplifies the immune response leading to the production of cytokines and the recruitment of other immune cells to the site of infection.

Pathways

CD14 interacts with the toll-like receptor signaling pathway notably involving TLR4. Upon LPS detection CD14 assists in the activation of TLR4 triggering downstream signaling cascades such as NF-κB and MAPK pathways. These pathways result in the transcription of inflammatory cytokines and are essential for the host defense mechanism. The protein complex involving CD14 further interacts with adaptor proteins like MyD88 and TRIF facilitating signal propagation and the immune system's ability to respond to pathogens.

Researchers have linked CD14 to sepsis and atherosclerosis. High levels of soluble CD14 (sCD14) are often observed in patients with sepsis indicating its participation in excessive systemic inflammation. In atherosclerosis CD14's role in recognizing LPS contributes to chronic inflammation and the development of plaques in arteries. Through these diseases CD14 connects with TLR4 emphasizing its impact on inflammatory responses and its potential as a therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Coreceptor for bacterial lipopolysaccharide (PubMed : 1698311, PubMed : 23264655). In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed : 20133493, PubMed : 22265692, PubMed : 23264655). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed : 8612135). Acts as a coreceptor for TLR2 : TLR6 heterodimer in response to diacylated lipopeptides and for TLR2 : TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (PubMed : 16880211). Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-) (PubMed : 23880187).

See full target information CD14

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in genetics 13:989459 PubMed36159969

2022

Bioinformatic analysis and experimental validation identified DNA methylation-Related biomarkers and immune-cell infiltration of atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Congjian Xu,Di Sun,Changmin Wei,Hao Chang

The Journal of clinical investigation 130:4624-4636 PubMed32516138

2020

Peripheral host T cells survive hematopoietic stem cell transplantation and promote graft-versus-host disease.

Applications

Unspecified application

Species

Unspecified reactive species

Sherrie J Divito,Anders T Aasebø,Tiago R Matos,Pei-Chen Hsieh,Matthew Collin,Christopher P Elco,John T O'Malley,Espen S Bækkevold,Henrik Reims,Tobias Gedde-Dahl,Michael Hagerstrom,Jude Hilaire,John W Lian,Edgar L Milford,Geraldine S Pinkus,Vincent T Ho,Robert J Soiffer,Haesook T Kim,Martin C Mihm,Jerome Ritz,Indira Guleria,Corey S Cutler,Rachael A Clark,Frode L Jahnsen,Thomas S Kupper

PloS one 13:e0206217 PubMed30485272

2018

Deficiency of the pattern-recognition receptor CD14 protects against joint pathology and functional decline in a murine model of osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Nisha Sambamurthy,Cheng Zhou,Vu Nguyen,Ryan Smalley,Kurt D Hankenson,George R Dodge,Carla R Scanzello

Molecular therapy. Nucleic acids 13:590-595 PubMed30439647

2018

Epistatic Association of CD14 and NOTCH2 Genetic Polymorphisms with Biliary Atresia in a Southern Chinese Population.

Applications

Unspecified application

Species

Unspecified reactive species

Zefeng Lin,Xiaoli Xie,Huiting Lin,Ming Fu,Liang Su,Yanlu Tong,Hongjiao Chen,Hezhen Wang,Jinglu Zhao,Huimin Xia,Yan Zhang,Ruizhong Zhang

Annals of transplantation 22:656-662 PubMed29097651

2017

A New Concept of the Old Inhibitor NSC 74859 in Alleviating Cardiac Allograft Rejection and Extending Allograft Survival in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yiquan Lai,Feng Kuang,Zhonggui Shan,Huaqing Liu

Autophagy 9:459-75 PubMed23380905

2013

ATG proteins mediate efferocytosis and suppress inflammation in mammary involution.

Applications

Unspecified application

Species

Unspecified reactive species

Irina Teplova,Fred Lozy,Sandy Price,Sukhwinder Singh,Nicola Barnard,Robert D Cardiff,Raymond B Birge,Vassiliki Karantza

View all publications

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