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Rabbit Polyclonal CD14 antibody. Suitable for WB, IHC-P and reacts with Pig samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody (AB231049), expandable thumbnail
  • Western blot - Anti-CD14 antibody (AB231049), expandable thumbnail
  • Western blot - Anti-CD14 antibody (AB231049), expandable thumbnail
  • Western blot - Anti-CD14 antibody (AB231049), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.12% PBS

Form
Liquid
Clonality
Polyclonal

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Pig
Tested
Tested

Tested
Tested

Species
Pig
Dilution info
0.5-2 µg/mL
Notes

-

Tested
Tested

Species
Pig
Dilution info
5-20 µg/mL
Notes

-

Associated Products

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Alternative names

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Rabbit Polyclonal CD14 antibody. Suitable for WB, IHC-P and reacts with Pig samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Polyclonal
Purification technique
Affinity purification Immunogen
Concentration
Loading...
Purification notes

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CD14 also known as cluster of differentiation 14 is a glycoprotein with a molecular mass of approximately 55 kDa. This protein is expressed mainly on the surface of macrophages monocytes and to a lesser extent on neutrophils. CD14 exists in two forms: membrane-bound (mCD14) and soluble (sCD14). It functions as a co-receptor along with TLR4 (Toll-like receptor 4) and MD-2 for the detection of bacterial lipopolysaccharide (LPS) which is an important component of the outer membrane of Gram-negative bacteria.

Biological function summary

This glycoprotein plays a significant role in the innate immune system by recognizing pathogen-associated molecular patterns (PAMPs). It is part of a receptor complex where CD14 acts alongside LPS binding protein (LPS-R) TLR4 and MD-2 to facilitate pro-inflammatory signaling in response to microbial invasion. By binding to LPS CD14 initiates and amplifies the immune response leading to the production of cytokines and the recruitment of other immune cells to the site of infection.

Pathways

CD14 interacts with the toll-like receptor signaling pathway notably involving TLR4. Upon LPS detection CD14 assists in the activation of TLR4 triggering downstream signaling cascades such as NF-κB and MAPK pathways. These pathways result in the transcription of inflammatory cytokines and are essential for the host defense mechanism. The protein complex involving CD14 further interacts with adaptor proteins like MyD88 and TRIF facilitating signal propagation and the immune system's ability to respond to pathogens.

Associated diseases and disorders

Researchers have linked CD14 to sepsis and atherosclerosis. High levels of soluble CD14 (sCD14) are often observed in patients with sepsis indicating its participation in excessive systemic inflammation. In atherosclerosis CD14's role in recognizing LPS contributes to chronic inflammation and the development of plaques in arteries. Through these diseases CD14 connects with TLR4 emphasizing its impact on inflammatory responses and its potential as a therapeutic target.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody (ab231049), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody (ab231049)

    Formalin-fixed, paraffin-embedded pig liver tissue stained for CD14 with ab231049 at 20 μg/ml in immunohistochemical analysis. DAB staining.

  • Western blot - Anti-CD14 antibody (ab231049), expandable thumbnail

    Western blot - Anti-CD14 antibody (ab231049)

    All lanes: Western blot - Anti-CD14 antibody (ab231049) at 2 µg/mL

    All lanes: Recombinant pig CD14 protein

    Developed using the ECL technique.

    Predicted band size: 40 kDa

  • Western blot - Anti-CD14 antibody (ab231049), expandable thumbnail

    Western blot - Anti-CD14 antibody (ab231049)

    All lanes: Western blot - Anti-CD14 antibody (ab231049) at 2 µg/mL

    All lanes: Pig lymph node

    Predicted band size: 40 kDa, 60 kDa

  • Western blot - Anti-CD14 antibody (ab231049), expandable thumbnail

    Western blot - Anti-CD14 antibody (ab231049)

    All lanes: Western blot - Anti-CD14 antibody (ab231049) at 2 µg/mL

    All lanes: Pig skin

    Predicted band size: 40 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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