Rabbit Polyclonal CD14 antibody. Suitable for WB, IHC-P and reacts with Pig samples.
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.12% PBS
WB | IHC-P | |
---|---|---|
Pig | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Pig | Dilution info 0.5-2 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Pig | Dilution info 5-20 µg/mL | Notes - |
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CD14 antigen, CD14 molecule, CD14_HUMAN, CD_antigen=CD14, LPS-R, Mo2, Monocyte differentiation antigen CD14, Monocyte differentiation antigen CD14 urinary form, Monocyte differentiation antigen CD14, membrane-bound form, Myeloid cell-specific leucine-rich glycoprotein
Rabbit Polyclonal CD14 antibody. Suitable for WB, IHC-P and reacts with Pig samples.
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.12% PBS
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
CD14 also known as cluster of differentiation 14 is a glycoprotein with a molecular mass of approximately 55 kDa. This protein is expressed mainly on the surface of macrophages monocytes and to a lesser extent on neutrophils. CD14 exists in two forms: membrane-bound (mCD14) and soluble (sCD14). It functions as a co-receptor along with TLR4 (Toll-like receptor 4) and MD-2 for the detection of bacterial lipopolysaccharide (LPS) which is an important component of the outer membrane of Gram-negative bacteria.
This glycoprotein plays a significant role in the innate immune system by recognizing pathogen-associated molecular patterns (PAMPs). It is part of a receptor complex where CD14 acts alongside LPS binding protein (LPS-R) TLR4 and MD-2 to facilitate pro-inflammatory signaling in response to microbial invasion. By binding to LPS CD14 initiates and amplifies the immune response leading to the production of cytokines and the recruitment of other immune cells to the site of infection.
CD14 interacts with the toll-like receptor signaling pathway notably involving TLR4. Upon LPS detection CD14 assists in the activation of TLR4 triggering downstream signaling cascades such as NF-κB and MAPK pathways. These pathways result in the transcription of inflammatory cytokines and are essential for the host defense mechanism. The protein complex involving CD14 further interacts with adaptor proteins like MyD88 and TRIF facilitating signal propagation and the immune system's ability to respond to pathogens.
Researchers have linked CD14 to sepsis and atherosclerosis. High levels of soluble CD14 (sCD14) are often observed in patients with sepsis indicating its participation in excessive systemic inflammation. In atherosclerosis CD14's role in recognizing LPS contributes to chronic inflammation and the development of plaques in arteries. Through these diseases CD14 connects with TLR4 emphasizing its impact on inflammatory responses and its potential as a therapeutic target.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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Formalin-fixed, paraffin-embedded pig liver tissue stained for CD14 with ab231049 at 20 μg/ml in immunohistochemical analysis. DAB staining.
All lanes: Western blot - Anti-CD14 antibody (ab231049) at 2 µg/mL
All lanes: Recombinant pig CD14 protein
Developed using the ECL technique.
Predicted band size: 40 kDa
All lanes: Western blot - Anti-CD14 antibody (ab231049) at 2 µg/mL
All lanes: Pig lymph node
Predicted band size: 40 kDa, 60 kDa
All lanes: Western blot - Anti-CD14 antibody (ab231049) at 2 µg/mL
All lanes: Pig skin
Predicted band size: 40 kDa
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