JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB231049

Anti-CD14 antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal CD14 antibody. Suitable for WB, IHC-P and reacts with Pig samples. Cited in 1 publication.
4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody (AB231049)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody (AB231049)

Formalin-fixed, paraffin-embedded pig liver tissue stained for CD14 with ab231049 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Western blot - Anti-CD14 antibody (AB231049)
  • WB

Unknown

Western blot - Anti-CD14 antibody (AB231049)

All lanes:

Western blot - Anti-CD14 antibody (ab231049) at 2 µg/mL

All lanes:

Pig lymph node

Predicted band size: 40 kDa,60 kDa

false

Western blot - Anti-CD14 antibody (AB231049)
  • WB

Supplier Data

Western blot - Anti-CD14 antibody (AB231049)

All lanes:

Western blot - Anti-CD14 antibody (ab231049) at 2 µg/mL

All lanes:

Recombinant pig CD14 protein

Predicted band size: 40 kDa

true

Western blot - Anti-CD14 antibody (AB231049)
  • WB

Unknown

Western blot - Anti-CD14 antibody (AB231049)

All lanes:

Western blot - Anti-CD14 antibody (ab231049) at 2 µg/mL

All lanes:

Pig skin

Predicted band size: 40 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Pig

Applications

WB, IHC-P

applications

style
left-column

Alternative Products

Primary Antibodies

AB183322

Anti-CD14 antibody [SP192]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [SP192] (AB183322)

  • 5
  • 5
Primary Antibodies

AB133503

Anti-CD14 antibody [EPR3652]

20ul selling size|RabMAb|Recombinant

Flow Cytometry - Anti-CD14 antibody [EPR3652] (AB133503)

  • 0
  • 0
Primary Antibodies

AB214438

Anti-CD14 antibody [EPR3653] - Low endotoxin, Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [EPR3653] - Low endotoxin, Azide free (AB214438)

  • 3
  • 1
Primary Antibodies

AB209971

Anti-CD14 antibody [EPR3652] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry - Anti-CD14 antibody [EPR3652] - BSA and Azide free (AB209971)

  • 0
  • 0
Primary Antibodies

AB133335

Anti-CD14 antibody [EPR3653]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [EPR3653] (AB133335)

  • 4
  • 1
Primary Antibodies

AB221678

Anti-CD14 antibody [EPR21847]

20ul selling size|RabMAb|Recombinant

Flow Cytometry - Anti-CD14 antibody [EPR21847] (AB221678)

  • 0
  • 0
Primary Antibodies

AB231852

Anti-CD14 antibody [EPR21847] - BSA and Azide free

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-CD14 antibody [EPR21847] - BSA and Azide free (AB231852)

  • 0
  • 0

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB134114

Anti-CD19 antibody [EPR5906]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Flow Cytometry (Intracellular) - Anti-CD19 antibody [EPR5906] (AB134114)

  • 4
  • 5
Primary Antibodies

AB955

Anti-CD68 antibody [KP1]

Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (AB955)

  • 4
  • 48
Primary Antibodies

AB52632

Anti-CD11c antibody [EP1347Y] - C-terminal

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11c antibody [EP1347Y] - C-terminal (AB52632)

  • 5
  • 18
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD14 also known as cluster of differentiation 14 is a glycoprotein with a molecular mass of approximately 55 kDa. This protein is expressed mainly on the surface of macrophages monocytes and to a lesser extent on neutrophils. CD14 exists in two forms: membrane-bound (mCD14) and soluble (sCD14). It functions as a co-receptor along with TLR4 (Toll-like receptor 4) and MD-2 for the detection of bacterial lipopolysaccharide (LPS) which is an important component of the outer membrane of Gram-negative bacteria.
Biological function summary

This glycoprotein plays a significant role in the innate immune system by recognizing pathogen-associated molecular patterns (PAMPs). It is part of a receptor complex where CD14 acts alongside LPS binding protein (LPS-R) TLR4 and MD-2 to facilitate pro-inflammatory signaling in response to microbial invasion. By binding to LPS CD14 initiates and amplifies the immune response leading to the production of cytokines and the recruitment of other immune cells to the site of infection.

Pathways

CD14 interacts with the toll-like receptor signaling pathway notably involving TLR4. Upon LPS detection CD14 assists in the activation of TLR4 triggering downstream signaling cascades such as NF-κB and MAPK pathways. These pathways result in the transcription of inflammatory cytokines and are essential for the host defense mechanism. The protein complex involving CD14 further interacts with adaptor proteins like MyD88 and TRIF facilitating signal propagation and the immune system's ability to respond to pathogens.

Researchers have linked CD14 to sepsis and atherosclerosis. High levels of soluble CD14 (sCD14) are often observed in patients with sepsis indicating its participation in excessive systemic inflammation. In atherosclerosis CD14's role in recognizing LPS contributes to chronic inflammation and the development of plaques in arteries. Through these diseases CD14 connects with TLR4 emphasizing its impact on inflammatory responses and its potential as a therapeutic target.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

See full target information CD14
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

NPJ Regenerative medicine 9:37 PubMed39639015

2024

Attenuation of skin injury by a MARCO targeting PLGA nanoparticle.

Applications

Unspecified application

Species

Unspecified reactive species

Ummiye V Onay,Dan Xu,Dauren Biyashev,Spencer T Evans,Michael M Demczuk,Tobias Neef,Joseph R Podojil,Sara Beddow,Nathan C Gianneschi,I Caroline Le Poole,Stephen D Miller,Kurt Q Lu
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com