AB45870

Anti-CD14 antibody

Name: Anti-CD14 antibody (ab45870) | Abcam
Brand: Abcam Limited
SKU: AB45870
Rating: 5 (1 reviews)

(1 Review)

(13 Publications)

Goat Polyclonal CD14 antibody. Suitable for WB and reacts with Human samples. Cited in 13 publications. Immunogen corresponding to Synthetic Peptide within Human CD14 aa 50-100.

View Alternative Names

CD14, Monocyte differentiation antigen CD14, My23 antigen, Myeloid cell-specific leucine-rich glycoprotein

1 Images

Supplier Data

Western blot - Anti-CD14 antibody (AB45870)

Primary incubation 1 hour at room temperature. Detected by chemiluminescence. RIPA buffer used.

All lanes:

Western blot - Anti-CD14 antibody (ab45870) at 2 µg/mL

Lane 1:

Human lymph node lysate at 35 µg

Lane 2:

Human lymph node lysate and immunizing peptide at 35 µg

Lane 3:

Human tonsil lysate at 35 µg

Lane 4:

Human tonsil lysate and Immunizing peptide at 35 µg

Predicted band size: 40 kDa

Observed band size: 55 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human CD14 aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

P08571

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "<p>A 1 hour primary incubation is recommended for this product. The observed molecular weight corresponds to earlier findings in literature with different antibodies (Funda et al, Infect Immun. 2001 Jun;69(6):3772-81; PMID: 11349042).</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD14 also known as cluster of differentiation 14 is a glycoprotein with a molecular mass of approximately 55 kDa. This protein is expressed mainly on the surface of macrophages monocytes and to a lesser extent on neutrophils. CD14 exists in two forms: membrane-bound (mCD14) and soluble (sCD14). It functions as a co-receptor along with TLR4 (Toll-like receptor 4) and MD-2 for the detection of bacterial lipopolysaccharide (LPS) which is an important component of the outer membrane of Gram-negative bacteria.

Biological function summary

This glycoprotein plays a significant role in the innate immune system by recognizing pathogen-associated molecular patterns (PAMPs). It is part of a receptor complex where CD14 acts alongside LPS binding protein (LPS-R) TLR4 and MD-2 to facilitate pro-inflammatory signaling in response to microbial invasion. By binding to LPS CD14 initiates and amplifies the immune response leading to the production of cytokines and the recruitment of other immune cells to the site of infection.

Pathways

CD14 interacts with the toll-like receptor signaling pathway notably involving TLR4. Upon LPS detection CD14 assists in the activation of TLR4 triggering downstream signaling cascades such as NF-κB and MAPK pathways. These pathways result in the transcription of inflammatory cytokines and are essential for the host defense mechanism. The protein complex involving CD14 further interacts with adaptor proteins like MyD88 and TRIF facilitating signal propagation and the immune system's ability to respond to pathogens.

Researchers have linked CD14 to sepsis and atherosclerosis. High levels of soluble CD14 (sCD14) are often observed in patients with sepsis indicating its participation in excessive systemic inflammation. In atherosclerosis CD14's role in recognizing LPS contributes to chronic inflammation and the development of plaques in arteries. Through these diseases CD14 connects with TLR4 emphasizing its impact on inflammatory responses and its potential as a therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Coreceptor for bacterial lipopolysaccharide (PubMed : 1698311, PubMed : 23264655). In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed : 20133493, PubMed : 22265692, PubMed : 23264655). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed : 8612135). Acts as a coreceptor for TLR2 : TLR6 heterodimer in response to diacylated lipopeptides and for TLR2 : TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (PubMed : 16880211). Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-) (PubMed : 23880187).

See full target information CD14

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Cellular and molecular life sciences : CMLS 79:542 PubMed36203068

2022

Phagocytosing differentiated cell-fragments is a novel mechanism for controlling somatic stem cell differentiation within a short time frame.

Applications

Unspecified application

Species

Unspecified reactive species

Shohei Wakao,Yo Oguma,Yoshihiro Kushida,Yasumasa Kuroda,Kazuki Tatsumi,Mari Dezawa

Biomedical reports 17:82 PubMed36158321

2022

Role of apoptosis inhibitor of macrophages in patients with IgG4‑related disease/autoimmune pancreatitis and the clinical characteristics associated with this condition.

Applications

Unspecified application

Species

Unspecified reactive species

Shiroh Tanoue,Shuji Kanmura,Makoto Hinokuchi,Shiho Arima,Fumisato Sasaki,Shinichi Hashimoto,Akio Ido

Endocrine 71:242-252 PubMed33010002

2020

Myeloid-derived suppressor cells in gastroenteropancreatic neuroendocrine neoplasms.

Applications

Unspecified application

Species

Unspecified reactive species

Man Liu,Yixuan Zhang,Luohai Chen,Yuan Lin,Qiao He,Yu Zeng,Minhu Chen,Jie Chen

Oncoimmunology 9:1773204 PubMed32923132

2020

Tumor stem cells fuse with monocytes to form highly invasive tumor-hybrid cells.

Applications

Unspecified application

Species

Unspecified reactive species

Luis Augusto Aguirre,Karla Montalbán-Hernández,José Avendaño-Ortiz,Elvira Marín,Roberto Lozano,Víctor Toledano,Laura Sánchez-Maroto,Verónica Terrón,Jaime Valentín,Elisa Pulido,José Carlos Casalvilla,Carolina Rubio,Luke Diekhorst,Fernando Laso-García,Carlos Del Fresno,Ana Collazo-Lorduy,Beatriz Jiménez-Munarriz,Paloma Gómez-Campelo,Emilio Llanos-González,María Fernández-Velasco,Carlos Rodríguez-Antolín,Rebeca Pérez de Diego,Ramón Cantero-Cid,Enrique Hernádez-Jimenez,Enrique Álvarez,Rocío Rosas,Blanca Dies López-Ayllón,Javier de Castro,Stefanie K Wculek,Carolina Cubillos-Zapata,Inmaculada Ibáñez de Cáceres,Prudencio Díaz-Agero,María Gutiérrez Fernández,María Paz de Miguel,David Sancho,Leon Schulte,Rosario Perona,Cristóbal Belda-Iniesta,Lisardo Boscá,Eduardo López-Collazo

Biotechnology and bioengineering 116:2652-2661 PubMed31180145

2019

Construction of a sensitive pyrogen-testing cell model by site-specific knock-in of multiple genes.

Applications

Unspecified application

Species

Unspecified reactive species

Ruobi Hu,Hui Li,Zhen Lei,Qing Han,Xiuyan Yu,Na Zhou,Xuehui Zhang,Yiqing Mao,Xi Wang,David M Irwin,Gang Niu,Huanran Tan

Biotechnology and bioengineering 116:1269-1279 PubMed30684361

2019

Application of a TLR overexpression cell model in pyrogen detection.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Han,Ruobi Hu,Hui Li,Zhen Lei,Xuehui Zhang,Xiuyan Yu,Qun Zhang,Yiqing Mao,Xi Wang,David M Irwin,Gang Niu,Huanran Tan

Cancer immunology, immunotherapy : CII 66:129-140 PubMed27858101

2016

A novel three-dimensional heterotypic spheroid model for the assessment of the activity of cancer immunotherapy agents.

Applications

IHC-P

Species

Human

Sylvia Herter,Laura Morra,Ramona Schlenker,Jitka Sulcova,Linda Fahrni,Inja Waldhauer,Steffi Lehmann,Timo Reisländer,Irina Agarkova,Jens M Kelm,Christian Klein,Pablo Umana,Marina Bacac

Biomedical research (Tokyo, Japan) 36:143-53 PubMed25876665

2015

CD14+ follicular dendritic cells in lymphoid follicles may play a role in the pathogenesis of IgG4-related disease.

Applications

Unspecified application

Species

Unspecified reactive species

Tomomi Satoh-Nakamura,Nozomu Kurose,Takafumi Kawanami,Takuji Nakamura,Haruka Iwao-Kawanami,Akio Nakajima,Miyuki Miki,Tomoyuki Sakai,Yoshimasa Fujita,Masao Tanaka,Toshihiro Fukushima,Yasufumi Masaki

Molecular medicine reports 12:2169-74 PubMed25845433

2015

Optimized generation of survivin-specific cytotoxic T lymphocytes against lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Li,Juanjuan Ding

Science translational medicine 6:250ra112 PubMed25143362

2014

Proteasome inhibitors increase missense mutated dysferlin in patients with muscular dystrophy.

Applications

Unspecified application

Species

Human

Bilal A Azakir,Beat Erne,Sabrina Di Fulvio,Guido Stirnimann,Michael Sinnreich

View all publications

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