Goat Polyclonal CD14 antibody. Suitable for WB and reacts with Human samples. Cited in 12 publications. Immunogen corresponding to Synthetic Peptide within Human CD14 aa 50-100.
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- Biomedical reports 17:822022Role of apoptosis inhibitor of macrophages in patients with IgG4‑related disease/autoimmune pancreatitis and the clinical characteristics associated with this condition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShiroh Tanoue,Shuji Kanmura,Makoto Hinokuchi,Shiho Arima,Fumisato Sasaki,Shinichi Hashimoto,Akio IdoPubMed 36158321
- Endocrine 71:242-2522020Myeloid-derived suppressor cells in gastroenteropancreatic neuroendocrine neoplasms.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMan Liu,Yixuan Zhang,Luohai Chen,Yuan Lin,Qiao He,Yu Zeng,Minhu Chen,Jie ChenPubMed 33010002
- Oncoimmunology 9:17732042020Tumor stem cells fuse with monocytes to form highly invasive tumor-hybrid cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLuis Augusto Aguirre et. al.PubMed 32923132
- Biotechnology and bioengineering 116:2652-26612019Construction of a sensitive pyrogen-testing cell model by site-specific knock-in of multiple genes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRuobi Hu,Hui Li,Zhen Lei,Qing Han,Xiuyan Yu,Na Zhou,Xuehui Zhang,Yiqing Mao,Xi Wang,David M Irwin,Gang Niu,Huanran TanPubMed 31180145
- Biotechnology and bioengineering 116:1269-12792019Application of a TLR overexpression cell model in pyrogen detection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQing Han et. al.PubMed 30684361
- Cancer immunology, immunotherapy : CII 66:129-1402016A novel three-dimensional heterotypic spheroid model for the assessment of the activity of cancer immunotherapy agents.Applications:IHC-PReactive species:HumanSylvia Herter,Laura Morra,Ramona Schlenker,Jitka Sulcova,Linda Fahrni,Inja Waldhauer,Steffi Lehmann,Timo Reisländer,Irina Agarkova,Jens M Kelm,Christian Klein,Pablo Umana,Marina BacacPubMed 27858101
- Biomedical research (Tokyo, Japan) 36:143-532015CD14+ follicular dendritic cells in lymphoid follicles may play a role in the pathogenesis of IgG4-related disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTomomi Satoh-Nakamura,Nozomu Kurose,Takafumi Kawanami,Takuji Nakamura,Haruka Iwao-Kawanami,Akio Nakajima,Miyuki Miki,Tomoyuki Sakai,Yoshimasa Fujita,Masao Tanaka,Toshihiro Fukushima,Yasufumi MasakiPubMed 25876665
- Molecular medicine reports 12:2169-742015Optimized generation of survivin-specific cytotoxic T lymphocytes against lung cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing Li et. al.PubMed 25845433
- Science translational medicine 6:250ra1122014Proteasome inhibitors increase missense mutated dysferlin in patients with muscular dystrophy.
- Annals of the rheumatic diseases 72:1382-92012Toll-like receptor-mediated, enhanced production of profibrotic TIMP-1 in monocytes from patients with systemic sclerosis: role of serum factors.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human CD14 aa 50-100. The exact immunogen used to generate this antibody is proprietary information. Database link P08571
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Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-3 µg/mL | Notes A 1 hour primary incubation is recommended for this product. The observed molecular weight corresponds to earlier findings in literature with different antibodies (Funda et al, Infect Immun. 2001 Jun;69(6):3772-81; PMID: 11349042). |
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Target data
Function
Coreceptor for bacterial lipopolysaccharide (PubMed:1698311, PubMed:23264655). In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed:20133493, PubMed:22265692, PubMed:23264655). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:8612135). Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (PubMed:16880211). Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-) (PubMed:23880187).
Alternative names
CD14, Monocyte differentiation antigen CD14, My23 antigen, Myeloid cell-specific leucine-rich glycoprotein
Recommended products
Goat Polyclonal CD14 antibody. Suitable for WB and reacts with Human samples. Cited in 12 publications. Immunogen corresponding to Synthetic Peptide within Human CD14 aa 50-100.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human CD14 aa 50-100. The exact immunogen used to generate this antibody is proprietary information. Database link P08571
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD14 also known as cluster of differentiation 14 is a glycoprotein with a molecular mass of approximately 55 kDa. This protein is expressed mainly on the surface of macrophages monocytes and to a lesser extent on neutrophils. CD14 exists in two forms: membrane-bound (mCD14) and soluble (sCD14). It functions as a co-receptor along with TLR4 (Toll-like receptor 4) and MD-2 for the detection of bacterial lipopolysaccharide (LPS) which is an important component of the outer membrane of Gram-negative bacteria.
- Biological function summary
This glycoprotein plays a significant role in the innate immune system by recognizing pathogen-associated molecular patterns (PAMPs). It is part of a receptor complex where CD14 acts alongside LPS binding protein (LPS-R) TLR4 and MD-2 to facilitate pro-inflammatory signaling in response to microbial invasion. By binding to LPS CD14 initiates and amplifies the immune response leading to the production of cytokines and the recruitment of other immune cells to the site of infection.
- Pathways
CD14 interacts with the toll-like receptor signaling pathway notably involving TLR4. Upon LPS detection CD14 assists in the activation of TLR4 triggering downstream signaling cascades such as NF-κB and MAPK pathways. These pathways result in the transcription of inflammatory cytokines and are essential for the host defense mechanism. The protein complex involving CD14 further interacts with adaptor proteins like MyD88 and TRIF facilitating signal propagation and the immune system's ability to respond to pathogens.
- Associated diseases and disorders
Researchers have linked CD14 to sepsis and atherosclerosis. High levels of soluble CD14 (sCD14) are often observed in patients with sepsis indicating its participation in excessive systemic inflammation. In atherosclerosis CD14's role in recognizing LPS contributes to chronic inflammation and the development of plaques in arteries. Through these diseases CD14 connects with TLR4 emphasizing its impact on inflammatory responses and its potential as a therapeutic target.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-CD14 antibody (ab45870)
Primary incubation 1 hour at room temperature. Detected by chemiluminescence. RIPA buffer used.
All lanes: Western blot - Anti-CD14 antibody (ab45870) at 2 µg/mL
Lane 1: Human lymph node lysate at 35 µg
Lane 2: Human lymph node lysate and immunizing peptide at 35 µg
Lane 3: Human tonsil lysate at 35 µg
Lane 4: Human tonsil lysate and Immunizing peptide at 35 µg
Predicted band size: 40 kDa
Observed band size: 55 kDa
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