AB314062

Anti-CD14 antibody [BLR187J] - BSA free

Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal CD14 antibody. Suitable for Flow Cyt, ICC, IHC-P, IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CD14 aa 1 to C-terminus.

View Alternative Names

CD14, Monocyte differentiation antigen CD14, My23 antigen, Myeloid cell-specific leucine-rich glycoprotein

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [BLR187J] - BSA free (AB314062)

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [BLR187J] - BSA free (AB314062)

Detection of human CD14 in tonsil by IHC. Antibody : Rabbit anti-CD14 recombinant monoclonal antibody [BLR187J]. Secondary : HRP-conjugated goat anti-rabbit IgG. Substrate : DAB

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [BLR187J] - BSA free (AB314062)

Detection of human CD14 in colon carcinoma TIL by IHC. Antibody : Rabbit anti-CD14 recombinant monoclonal antibody [BLR187J] (A700-187 lot 1). Secondary : HRPconjugated goat anti-rabbit IgG (A120-501P). Substrate : DAB

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Immunocytochemistry - Anti-CD14 antibody [BLR187J] - BSA free (AB314062)

Detection of human CD14 in MUTZ-3 cells by ICC. Antibody : Rabbit anti-CD14 recombinant monoclonal antibody [BLR187J]. Secondary : HRPconjugated goat anti-rabbit IgG . Substrate : DAB

Flow Cytometry - Anti-CD14 antibody [BLR187J] - BSA free (AB314062)

Flow Cyt

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Flow Cytometry - Anti-CD14 antibody [BLR187J] - BSA free (AB314062)

Detection of human CD14 (right) in PBMCs by flow cytometry. Antibody : Rabbit anti-CD14 recombinant monoclonal [BLR187J] or isotype control (left). Secondary : 488-conjugated goat antirabbit IgG. Analysis performed on lymphocyte and monocyte gated population.

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Immunoprecipitation - Anti-CD14 antibody [BLR187J] - BSA free (AB314062)

Samples : Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MUTZ-3 cells prepared using NETN lysis buffer. Antibody : Rabbit antiCD14 recombinant monoclonal antibody [BLR187J] used at 20 µl/mg lysate. For blotting immunoprecipitated CD14, was used at 1 : 1000. Chemiluminescence with an exposure time of 3 minutes.

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Western blot - Anti-CD14 antibody [BLR187J] - BSA free (AB314062)

Samples : Whole cell lysate (50 µg) from THP-1 +LPS, HEK293T, MUTZ-3, HeLa, and Jurkat cells prepared using NETN lysis buffer. Antibody : Rabbit anti-CD14 recombinant monoclonal antibody [BLR187J] used at 1 : 1000. Secondary : HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 minutes. Lower Panel : Rabbit anti-COPB2 antibody

All lanes:

Western blot - Anti-CD14 antibody [BLR187J] - BSA free (ab314062) at 1/1000 dilution

Lane 1:

THP-1 +LPS Whole cell lysate at 50 µg

Lane 2:

HEK293T Whole cell lysate at 50 µg

Lane 3:

MUTZ-3 Whole cell lysate at 50 µg

Lane 4:

HeLa Whole cell lysate at 50 µg

Lane 5:

Jurkat Whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR187J

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, IHC-P, ICC, WB, Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human CD14 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P08571

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD14 also known as cluster of differentiation 14 is a glycoprotein with a molecular mass of approximately 55 kDa. This protein is expressed mainly on the surface of macrophages monocytes and to a lesser extent on neutrophils. CD14 exists in two forms: membrane-bound (mCD14) and soluble (sCD14). It functions as a co-receptor along with TLR4 (Toll-like receptor 4) and MD-2 for the detection of bacterial lipopolysaccharide (LPS) which is an important component of the outer membrane of Gram-negative bacteria.

Biological function summary

This glycoprotein plays a significant role in the innate immune system by recognizing pathogen-associated molecular patterns (PAMPs). It is part of a receptor complex where CD14 acts alongside LPS binding protein (LPS-R) TLR4 and MD-2 to facilitate pro-inflammatory signaling in response to microbial invasion. By binding to LPS CD14 initiates and amplifies the immune response leading to the production of cytokines and the recruitment of other immune cells to the site of infection.

Pathways

CD14 interacts with the toll-like receptor signaling pathway notably involving TLR4. Upon LPS detection CD14 assists in the activation of TLR4 triggering downstream signaling cascades such as NF-κB and MAPK pathways. These pathways result in the transcription of inflammatory cytokines and are essential for the host defense mechanism. The protein complex involving CD14 further interacts with adaptor proteins like MyD88 and TRIF facilitating signal propagation and the immune system's ability to respond to pathogens.

Researchers have linked CD14 to sepsis and atherosclerosis. High levels of soluble CD14 (sCD14) are often observed in patients with sepsis indicating its participation in excessive systemic inflammation. In atherosclerosis CD14's role in recognizing LPS contributes to chronic inflammation and the development of plaques in arteries. Through these diseases CD14 connects with TLR4 emphasizing its impact on inflammatory responses and its potential as a therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Coreceptor for bacterial lipopolysaccharide (PubMed : 1698311, PubMed : 23264655). In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed : 20133493, PubMed : 22265692, PubMed : 23264655). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed : 8612135). Acts as a coreceptor for TLR2 : TLR6 heterodimer in response to diacylated lipopeptides and for TLR2 : TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (PubMed : 16880211). Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-) (PubMed : 23880187).

See full target information CD14

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 30:456 PubMed40762017

2025

Acute myeloid leukemia with plasmacytoid dendritic cell proliferation: A case report and literature review.

Applications

Unspecified application

Species

Unspecified reactive species

Zhixin Pei,Yi Zhang,Han Xu,Pei Pei,Zhengyang Zhang,Hongxia Wang,Bei Zhang,Junjun Bai,Yingxin Zhao,Jingjing Gu,Zhiyu Fang,Miaomiao Liu,Qinglin Song

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD14 antibody [BLR187J] - BSA free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [BLR187J] - BSA free (AB314062)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [BLR187J] - BSA free (AB314062)

Immunocytochemistry - Anti-CD14 antibody [BLR187J] - BSA free (AB314062)

Flow Cytometry - Anti-CD14 antibody [BLR187J] - BSA free (AB314062)

Immunoprecipitation - Anti-CD14 antibody [BLR187J] - BSA free (AB314062)

Western blot - Anti-CD14 antibody [BLR187J] - BSA free (AB314062)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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