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Rabbit Recombinant Monoclonal CD14 antibody. Suitable for Flow Cyt, ICC, IHC-P, IP, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CD14 aa 1 to C-terminus.

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Images

Western blot - Anti-CD14 antibody [BLR187J] - BSA free (AB314062), expandable thumbnail
  • Immunoprecipitation - Anti-CD14 antibody [BLR187J] - BSA free (AB314062), expandable thumbnail
  • Immunocytochemistry - Anti-CD14 antibody [BLR187J] - BSA free (AB314062), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [BLR187J] - BSA free (AB314062), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [BLR187J] - BSA free (AB314062), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human CD14 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P08571

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytICCIHC-PIPWB
Human
Tested
Tested
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

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Tested
Tested

Species
Human
Dilution info
-
Notes

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Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Target data

Function

Coreceptor for bacterial lipopolysaccharide (PubMed:1698311, PubMed:23264655). In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed:20133493, PubMed:22265692, PubMed:23264655). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:8612135). Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (PubMed:16880211). Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-) (PubMed:23880187).

Alternative names

CD14, Monocyte differentiation antigen CD14, My23 antigen, Myeloid cell-specific leucine-rich glycoprotein

Recommended products

Rabbit Recombinant Monoclonal CD14 antibody. Suitable for Flow Cyt, ICC, IHC-P, IP, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CD14 aa 1 to C-terminus.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Human CD14 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P08571
Clone number
BLR187J
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
Up to 12 months
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CD14 also known as cluster of differentiation 14 is a glycoprotein with a molecular mass of approximately 55 kDa. This protein is expressed mainly on the surface of macrophages monocytes and to a lesser extent on neutrophils. CD14 exists in two forms: membrane-bound (mCD14) and soluble (sCD14). It functions as a co-receptor along with TLR4 (Toll-like receptor 4) and MD-2 for the detection of bacterial lipopolysaccharide (LPS) which is an important component of the outer membrane of Gram-negative bacteria.

Biological function summary

This glycoprotein plays a significant role in the innate immune system by recognizing pathogen-associated molecular patterns (PAMPs). It is part of a receptor complex where CD14 acts alongside LPS binding protein (LPS-R) TLR4 and MD-2 to facilitate pro-inflammatory signaling in response to microbial invasion. By binding to LPS CD14 initiates and amplifies the immune response leading to the production of cytokines and the recruitment of other immune cells to the site of infection.

Pathways

CD14 interacts with the toll-like receptor signaling pathway notably involving TLR4. Upon LPS detection CD14 assists in the activation of TLR4 triggering downstream signaling cascades such as NF-κB and MAPK pathways. These pathways result in the transcription of inflammatory cytokines and are essential for the host defense mechanism. The protein complex involving CD14 further interacts with adaptor proteins like MyD88 and TRIF facilitating signal propagation and the immune system's ability to respond to pathogens.

Associated diseases and disorders

Researchers have linked CD14 to sepsis and atherosclerosis. High levels of soluble CD14 (sCD14) are often observed in patients with sepsis indicating its participation in excessive systemic inflammation. In atherosclerosis CD14's role in recognizing LPS contributes to chronic inflammation and the development of plaques in arteries. Through these diseases CD14 connects with TLR4 emphasizing its impact on inflammatory responses and its potential as a therapeutic target.

Product promise

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6 product images

  • Western blot - Anti-CD14 antibody [BLR187J] - BSA free (ab314062), expandable thumbnail

    Western blot - Anti-CD14 antibody [BLR187J] - BSA free (ab314062)

    Samples: Whole cell lysate (50 µg) from THP-1 +LPS, HEK293T, MUTZ-3, HeLa, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD14 recombinant monoclonal antibody [BLR187J] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody

    All lanes: Western blot - Anti-CD14 antibody [BLR187J] - BSA free (ab314062) at 1/1000 dilution

    Lane 1: THP-1 +LPS Whole cell lysate at 50 µg

    Lane 2: HEK293T Whole cell lysate at 50 µg

    Lane 3: MUTZ-3 Whole cell lysate at 50 µg

    Lane 4: HeLa Whole cell lysate at 50 µg

    Lane 5: Jurkat Whole cell lysate at 50 µg

    Secondary

    All lanes: HRP-conjugated goat anti-rabbit IgG

  • Immunoprecipitation - Anti-CD14 antibody [BLR187J] - BSA free (ab314062), expandable thumbnail

    Immunoprecipitation - Anti-CD14 antibody [BLR187J] - BSA free (ab314062)

    Samples:Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MUTZ-3 cells prepared using NETN lysis buffer. Antibody:Rabbit antiCD14 recombinant monoclonal antibody [BLR187J] used at 20 µl/mg lysate. For blotting immunoprecipitated CD14, was used at 1:1000. Chemiluminescence with an exposure time of 3 minutes.

  • Immunocytochemistry - Anti-CD14 antibody [BLR187J] - BSA free (ab314062), expandable thumbnail

    Immunocytochemistry - Anti-CD14 antibody [BLR187J] - BSA free (ab314062)

    Detection of human CD14 in MUTZ-3 cells by ICC. Antibody:Rabbit anti-CD14 recombinant monoclonal antibody [BLR187J]. Secondary:HRPconjugated goat anti-rabbit IgG . Substrate: DAB

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [BLR187J] - BSA free (ab314062), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [BLR187J] - BSA free (ab314062)

    Detection of human CD14 in tonsil by IHC. Antibody: Rabbit anti-CD14 recombinant monoclonal antibody [BLR187J]. Secondary:HRP-conjugated goat anti-rabbit IgG. Substrate:DAB

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [BLR187J] - BSA free (ab314062), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [BLR187J] - BSA free (ab314062)

    Detection of human CD14 in colon carcinoma TIL by IHC. Antibody:Rabbit anti-CD14 recombinant monoclonal antibody [BLR187J] (A700-187 lot 1). Secondary:HRPconjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB

  • Flow Cytometry - Anti-CD14 antibody [BLR187J] - BSA free (ab314062), expandable thumbnail

    Flow Cytometry - Anti-CD14 antibody [BLR187J] - BSA free (ab314062)

    Detection of human CD14 (right) in PBMCs by flow cytometry. Antibody: Rabbit anti-CD14 recombinant monoclonal [BLR187J] or isotype control (left). Secondary: 488-conjugated goat antirabbit IgG. Analysis performed on lymphocyte and monocyte gated population.

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Product protocols

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