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AB6083

Anti-CD14 antibody [MEM-18] - Low endotoxin, Azide free

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(9 Publications)

Mouse Monoclonal CD14 antibody. Carrier free. Suitable for Flow Cyt and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human CD14.

View Alternative Names

CD14, Monocyte differentiation antigen CD14, My23 antigen, Myeloid cell-specific leucine-rich glycoprotein

1 Images
Flow Cytometry - Anti-CD14 antibody [MEM-18] - Low endotoxin, Azide free (AB6083)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-CD14 antibody [MEM-18] - Low endotoxin, Azide free (AB6083)

Human peripheral blood cells stained with ab6083 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID : 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lysed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab6083, 0.01μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4°C. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • 660 APC

    APC Anti-CD14 antibody [MEM-18]

  • 519 FITC

    FITC Anti-CD14 antibody [MEM-18]

  • Biotin

    Biotin Anti-CD14 antibody [MEM-18]

  • 723 Alexa Fluor® 700

    Alexa Fluor® 700 Anti-CD14 antibody [MEM-18]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM-18

Isotype

IgG1

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Native Full Length Protein corresponding to Human CD14.

P08571

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.01 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" }, "Monkey": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Primates": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD14 also known as cluster of differentiation 14 is a glycoprotein with a molecular mass of approximately 55 kDa. This protein is expressed mainly on the surface of macrophages monocytes and to a lesser extent on neutrophils. CD14 exists in two forms: membrane-bound (mCD14) and soluble (sCD14). It functions as a co-receptor along with TLR4 (Toll-like receptor 4) and MD-2 for the detection of bacterial lipopolysaccharide (LPS) which is an important component of the outer membrane of Gram-negative bacteria.
Biological function summary

This glycoprotein plays a significant role in the innate immune system by recognizing pathogen-associated molecular patterns (PAMPs). It is part of a receptor complex where CD14 acts alongside LPS binding protein (LPS-R) TLR4 and MD-2 to facilitate pro-inflammatory signaling in response to microbial invasion. By binding to LPS CD14 initiates and amplifies the immune response leading to the production of cytokines and the recruitment of other immune cells to the site of infection.

Pathways

CD14 interacts with the toll-like receptor signaling pathway notably involving TLR4. Upon LPS detection CD14 assists in the activation of TLR4 triggering downstream signaling cascades such as NF-κB and MAPK pathways. These pathways result in the transcription of inflammatory cytokines and are essential for the host defense mechanism. The protein complex involving CD14 further interacts with adaptor proteins like MyD88 and TRIF facilitating signal propagation and the immune system's ability to respond to pathogens.

Researchers have linked CD14 to sepsis and atherosclerosis. High levels of soluble CD14 (sCD14) are often observed in patients with sepsis indicating its participation in excessive systemic inflammation. In atherosclerosis CD14's role in recognizing LPS contributes to chronic inflammation and the development of plaques in arteries. Through these diseases CD14 connects with TLR4 emphasizing its impact on inflammatory responses and its potential as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Coreceptor for bacterial lipopolysaccharide (PubMed : 1698311, PubMed : 23264655). In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed : 20133493, PubMed : 22265692, PubMed : 23264655). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed : 8612135). Acts as a coreceptor for TLR2 : TLR6 heterodimer in response to diacylated lipopeptides and for TLR2 : TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (PubMed : 16880211). Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-) (PubMed : 23880187).
See full target information CD14
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cellular and infection microbiology 13:1125901 PubMed37009499

2023

Mechanism of resistance to phagocytosis and pulmonary persistence in mucoid .

Applications

Unspecified application

Species

Unspecified reactive species

Warren J Rowe,Deborah A Lebman,Dennis E Ohman

Journal of virology 90:6187-6199 PubMed27099316

2016

Hepatitis B Virus Surface Antigen Activates Myeloid Dendritic Cells via a Soluble CD14-Dependent Mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Nadine van Montfoort,Evelyn van der Aa,Aniek van den Bosch,Hilde Brouwers,Thomas Vanwolleghem,Harry L A Janssen,Hassan Javanbakht,Sonja I Buschow,Andrea M Woltman

Cell and tissue banking 17:137-45 PubMed26220398

2015

Cryopreserved CD90+ cells obtained from mobilized peripheral blood in sheep: a new source of mesenchymal stem cells for preclinical applications.

Applications

IF

Species

Unspecified reactive species

Carlos Landa-Solís,Julio Granados-Montiel,Anell Olivos-Meza,Carmina Ortega-Sánchez,Mónica Cruz-Lemini,Cecilia Hernández-Flores,María Eugenia Chang-González,Ricardo Gómez García,Brenda Olivos-Díaz,María Cristina Velasquillo-Martínez,Carlos Pineda,Clemente Ibarra

International immunology 23:185-93 PubMed21393634

2011

Human anti-microbial cathelicidin peptide LL-37 suppresses the LPS-induced apoptosis of endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kaori Suzuki,Taisuke Murakami,Kyoko Kuwahara-Arai,Hiroshi Tamura,Keiichi Hiramatsu,Isao Nagaoka

Clinical and vaccine immunology : CVI 16:1750-7 PubMed19793902

2009

Mycoplasma genitalium-derived lipid-associated membrane proteins activate NF-kappaB through toll-like receptors 1, 2, and 6 and CD14 in a MyD88-dependent pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jun He,Xiaoxing You,Yanhua Zeng,Minjun Yu,Lingling Zuo,Yimou Wu

Cytotechnology 54:121-33 PubMed19003027

2007

Isolation of progenitor cells from cord blood using adhesion matrices.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Maurice,Samer Srouji,Erella Livne

European journal of immunology 24:1779-84 PubMed7519994

1994

Soluble CD14 from urine copurifies with a potent inducer of cytokines.

Applications

Unspecified application

Species

Unspecified reactive species

A Sundan,T Gullstein-Jahr,M Otterlei,L Ryan,V Bazil,S D Wright,T Espevik

Molecular immunology 26:657-62 PubMed2779588

1989

Structural relationship between the soluble and membrane-bound forms of human monocyte surface glycoprotein CD14.

Applications

Unspecified application

Species

Unspecified reactive species

V Bazil,M Baudys,I Hilgert,I Stefanová,M G Low,J Zbrozek,V Horejsí

European journal of immunology 16:1583-9 PubMed3493149

1986

Biochemical characterization of a soluble form of the 53-kDa monocyte surface antigen.

Applications

Unspecified application

Species

Unspecified reactive species

V Bazil,V Horejsí,M Baudys,H Kristofová,J L Strominger,W Kostka,I Hilgert
View all publications
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