Mouse Monoclonal CD14 antibody. Carrier free. Suitable for Flow Cyt and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human CD14.
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![Flow Cytometry - Anti-CD14 antibody [MEM-18] - Low endotoxin, Azide free (AB6083), expandable thumbnail](https://content.abcam.com/products/images/cd14-antibody-mem-18-low-endotoxin-azide-free-ab6083--flow-cytometry-img74669.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CD14 antibody [MEM-18] - Low endotoxin, Azide free (AB6083)")
Publications
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- Frontiers in cellular and infection microbiology 13:11259012023Mechanism of resistance to phagocytosis and pulmonary persistence in mucoid .Applications:Unspecified applicationReactive species:Unspecified reactive speciesWarren J Rowe,Deborah A Lebman,Dennis E OhmanPubMed 37009499
- Journal of virology 90:6187-61992016Hepatitis B Virus Surface Antigen Activates Myeloid Dendritic Cells via a Soluble CD14-Dependent Mechanism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNadine van Montfoort et. al.PubMed 27099316
- Cell and tissue banking 17:137-452015Cryopreserved CD90+ cells obtained from mobilized peripheral blood in sheep: a new source of mesenchymal stem cells for preclinical applications.Applications:IFReactive species:Unspecified reactive speciesCarlos Landa-Solís et. al.PubMed 26220398
- International immunology 23:185-932011Human anti-microbial cathelicidin peptide LL-37 suppresses the LPS-induced apoptosis of endothelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKaori Suzuki,Taisuke Murakami,Kyoko Kuwahara-Arai,Hiroshi Tamura,Keiichi Hiramatsu,Isao NagaokaPubMed 21393634
- Clinical and vaccine immunology : CVI 16:1750-72009Mycoplasma genitalium-derived lipid-associated membrane proteins activate NF-kappaB through toll-like receptors 1, 2, and 6 and CD14 in a MyD88-dependent pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun He,Xiaoxing You,Yanhua Zeng,Minjun Yu,Lingling Zuo,Yimou WuPubMed 19793902
- Cytotechnology 54:121-332007Isolation of progenitor cells from cord blood using adhesion matrices.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSarah Maurice et. al.PubMed 19003027
- European journal of immunology 24:1779-841994Soluble CD14 from urine copurifies with a potent inducer of cytokines.Applications:Unspecified applicationReactive species:Unspecified reactive speciesA Sundan,T Gullstein-Jahr,M Otterlei,L Ryan,V Bazil,S D Wright,T EspevikPubMed 7519994
- Molecular immunology 26:657-621989Structural relationship between the soluble and membrane-bound forms of human monocyte surface glycoprotein CD14.Applications:Unspecified applicationReactive species:Unspecified reactive speciesV Bazil,M Baudys,I Hilgert,I Stefanová,M G Low,J Zbrozek,V HorejsíPubMed 2779588
- European journal of immunology 16:1583-91986Biochemical characterization of a soluble form of the 53-kDa monocyte surface antigen.Applications:Unspecified applicationReactive species:Unspecified reactive speciesV Bazil,V Horejsí,M Baudys,H Kristofová,J L Strominger,W Kostka,I HilgertPubMed 3493149
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Native Full Length Protein corresponding to Human CD14. Database link P08571
Reactivity data
Select an application| Flow Cyt | |
|---|---|
| Human | Tested |
| Monkey | Predicted |
| Primates | Predicted |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.01 µg for 106 Cells | Notes ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Monkey, Primates | Dilution info - | Notes - |
Associated Products
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Target data
Function
Coreceptor for bacterial lipopolysaccharide (PubMed:1698311, PubMed:23264655). In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed:20133493, PubMed:22265692, PubMed:23264655). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:8612135). Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (PubMed:16880211). Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-) (PubMed:23880187).
Alternative names
CD14, Monocyte differentiation antigen CD14, My23 antigen, Myeloid cell-specific leucine-rich glycoprotein
Recommended products
Mouse Monoclonal CD14 antibody. Carrier free. Suitable for Flow Cyt and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human CD14.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Native Full Length Protein corresponding to Human CD14. Database link P08571
- Carrier free
- Yes
- Clone number
- MEM-18
- Purity
- Tissue culture supernatant
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD14 also known as cluster of differentiation 14 is a glycoprotein with a molecular mass of approximately 55 kDa. This protein is expressed mainly on the surface of macrophages monocytes and to a lesser extent on neutrophils. CD14 exists in two forms: membrane-bound (mCD14) and soluble (sCD14). It functions as a co-receptor along with TLR4 (Toll-like receptor 4) and MD-2 for the detection of bacterial lipopolysaccharide (LPS) which is an important component of the outer membrane of Gram-negative bacteria.
- Biological function summary
This glycoprotein plays a significant role in the innate immune system by recognizing pathogen-associated molecular patterns (PAMPs). It is part of a receptor complex where CD14 acts alongside LPS binding protein (LPS-R) TLR4 and MD-2 to facilitate pro-inflammatory signaling in response to microbial invasion. By binding to LPS CD14 initiates and amplifies the immune response leading to the production of cytokines and the recruitment of other immune cells to the site of infection.
- Pathways
CD14 interacts with the toll-like receptor signaling pathway notably involving TLR4. Upon LPS detection CD14 assists in the activation of TLR4 triggering downstream signaling cascades such as NF-κB and MAPK pathways. These pathways result in the transcription of inflammatory cytokines and are essential for the host defense mechanism. The protein complex involving CD14 further interacts with adaptor proteins like MyD88 and TRIF facilitating signal propagation and the immune system's ability to respond to pathogens.
- Associated diseases and disorders
Researchers have linked CD14 to sepsis and atherosclerosis. High levels of soluble CD14 (sCD14) are often observed in patients with sepsis indicating its participation in excessive systemic inflammation. In atherosclerosis CD14's role in recognizing LPS contributes to chronic inflammation and the development of plaques in arteries. Through these diseases CD14 connects with TLR4 emphasizing its impact on inflammatory responses and its potential as a therapeutic target.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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1 product image
![Flow Cytometry - Anti-CD14 antibody [MEM-18] - Low endotoxin, Azide free (ab6083), expandable thumbnail](https://content.abcam.com/products/images/cd14-antibody-mem-18-low-endotoxin-azide-free-ab6083--flow-cytometry-img74669.jpg "Flow Cytometry - Anti-CD14 antibody [MEM-18] - Low endotoxin, Azide free (ab6083)")
Flow Cytometry - Anti-CD14 antibody [MEM-18] - Low endotoxin, Azide free (ab6083)
Human peripheral blood cells stained with ab6083 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab6083, 0.01μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) at 1/2000 dilution for 30 min at 4°C. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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