Anti-CD14 antibody [SP192] - BSA and Azide free

• Flow Cyt

Lab

Flow Cytometry - Anti-CD14 antibody [SP192] - BSA and Azide free (AB230903)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183322).

Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab183322 (right) or Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (left). PBMCs were incubated for 30 min on ice in 1x PBS containing 10 µg/ml human IgG and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab183322 or Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (1x 10⁶ in 100 µl at 0.008 μg/ml) for 30min on ice. The cells were simultaneously stained with CD19.

The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 for 30min on ice

Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on live cells.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [SP192] - BSA and Azide free (AB230903)

Immunohistochemical analysis of paraffin embedded human tonsil tissue labeling CD14 with ab183322 at 1/100.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab183322).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [SP192] - BSA and Azide free (AB230903)

Immunohistochemical analysis of paraffin embedded Human esophagus tissue labeling CD14 with ab183322 at 1/100.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183322).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [SP192] - BSA and Azide free (AB230903)

Immunohistochemical analysis of paraffin embedded Human liver tissue labeling CD14 with ab183322 at 1/100.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183322).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [SP192] - BSA and Azide free (AB230903)

Immunohistochemical analysis of paraffin embedded Human spleen tissue labeling CD14 with ab183322 at 1/100.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183322).

• Flow Cyt

Unknown

Flow Cytometry - Anti-CD14 antibody [SP192] - BSA and Azide free (AB230903)

Flow Cytometry analysis of human PBMC (human peripheral blood mononuclear cell) cells labeling CD14 with purified ab183322 at 1 : 200 dilution (0.805 μg/ml) - Red. A Goat anti rabbit IgG (Dylight® 488, ab98462) secondary antibody was used at 1 : 2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230903)

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [SP192] - BSA and Azide free (AB230903)

Immunohistochemical analysis of paraffin embedded Human lung tissue labeling CD14 with ab183322 at 1/100.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183322).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [SP192] - BSA and Azide free (AB230903)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling CD14 with ab183322 at 1/100 dilution (1.61 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on stromal cells in the human colon, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab183322 for 10 mins at room temperature. This image was generated using ab183322, the same clone, but with a different buffer formulation.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [SP192] - BSA and Azide free (AB230903)

Immunohistochemical analysis of paraffin embedded Human HK lymphoma tissue labeling CD14 with ab183322 at 1/100.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183322).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [SP192] - BSA and Azide free (AB230903)

Immunohistochemical analysis of paraffin embedded Human B cell lymphoma tissue labeling CD14 with ab183322 at 1/100.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183322).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [SP192] - BSA and Azide free (AB230903)

Immunohistochemical analysis of paraffin embedded Human colon tissue labeling CD14 with ab183322 at 1/100.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183322).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [SP192] - BSA and Azide free (AB230903)

Immunohistochemical analysis of paraffin embedded Human bladder tissue labeling CD14 with ab183322 at 1/100.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183322).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [SP192] - BSA and Azide free (AB230903)

Immunohistochemical analysis of paraffin embedded Human bone marrow tissue labeling CD14 with ab183322 at 1/100.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183322).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [SP192] - BSA and Azide free (AB230903)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling CD14 with ab183322 at 1 : 100 dilution (1.61 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human tonsil, performed on a Leica Biosystems BOND™ RX instrument. The section was incubated with ab183322 for 10 mins at room temperature.

This image was generated using ab183322, the same clone, but with a different buffer formulation.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [SP192] - BSA and Azide free (AB230903)

Immunohistochemical analysis of paraffin embedded Human endometrium tissue labeling CD14 with ab183322 at 1/100.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183322).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [SP192] - BSA and Azide free (AB230903)

Immunohistochemical analysis of paraffin embedded Human colon adenocarcinoma tissue labeling CD14 with ab183322 at 1/100.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183322).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [SP192] - BSA and Azide free (AB230903)

Immunohistochemical analysis of formalin-fixed paraffin-embedded human tonsil labelling CD14 with ab230903 at a concentration of 0.5µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with an OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was conducted for 32min with ULTRA cell conditioning solution (CC1 pH8.5). ab220903 anti CD14 antibody was incubated at 37°C for 16min. Sections were counterstained is with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [SP192] - BSA and Azide free (AB230903)

Immunohistochemical analysis of paraffin embedded Human thymus tissue labeling CD14 with ab183322 at 1/100.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183322).

• Flow Cyt

Lab

Flow Cytometry - Anti-CD14 antibody [SP192] - BSA and Azide free (AB230903)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183322).

Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab183322 (right) or Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (left). PBMCs were incubated for 30 mins on ice in 1x PBS containing 10 µg/ml human IgG and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab183322 or Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (1x 10⁶ in 100 µl at 0.008 μg/ml) for 30 mins on ice. The cells were simultaneously stained with CD19.

The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed was incubated at 1/4000 dilutionfor 30 mins on ice

Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on CD19-ve.