Rabbit Polyclonal CD146 antibody. C-terminal. Suitable for WB, IHC-P, ICC/IF and reacts with Rat, Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MCAM.
IgG
Rabbit
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
WB | IHC-P | ICC/IF | |
---|---|---|---|
Human | Tested | Expected | Tested |
Mouse | Expected | Tested | Expected |
Rat | Tested | Expected | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info 1/500 - 1/3000 | Notes - |
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/100 - 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Select an associated product type
Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as a surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.
MUC18, MCAM, Cell surface glycoprotein MUC18, Cell surface glycoprotein P1H12, Melanoma cell adhesion molecule, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, S-endo 1 endothelial-associated antigen
Rabbit Polyclonal CD146 antibody. C-terminal. Suitable for WB, IHC-P, ICC/IF and reacts with Rat, Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MCAM.
IgG
Rabbit
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
CD146 also known as MCAM or MUC18 is a cell adhesion molecule that belongs to the immunoglobulin superfamily. With a mass of approximately 113 kDa CD146 is expressed mainly on the surface of endothelial cells but it can also be found on smooth muscle cells some T-cells and in certain cancerous tissues. This protein plays a role in cell-cell adhesion contributing to the integrity and signaling functions within tissue microenvironments.
CD146 influences the migration and organization of cells within the vascular system. It serves as a component of cell adhesion complexes facilitating interactions between endothelial cells and other cell types. Additionally CD146 contributes to angiogenesis the process by which new blood vessels form from existing vasculature which is critical during tissue development and repair. This function makes it an important element in maintaining normal physiological processes.
CD146 participates in the Wnt/β-catenin and MAPK signaling pathways which are vital in cell proliferation and differentiation. Through the Wnt/β-catenin pathway CD146 interacts with other proteins like Frizzled receptors to regulate gene transcription. In the MAPK pathway it is linked with signaling cascades that involve proteins such as ERK leading to cellular responses necessary for growth and response to external stimuli.
CD146 has been implicated in melanoma progression and cardiovascular diseases. Its overexpression in melanoma associates it with increased tumor growth and metastasis often involving interactions with other adhesion molecules like integrins. In cardiovascular diseases CD146 relates to atherosclerosis as its expression on endothelial cells can promote inflammatory responses. Understanding these connections provides insights into potential therapeutic targets for treating these conditions.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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Terms & Conditions.
Paraffin-embedded J5 xenograft stained for CD146 using ab228540 at 1/500 dilution in immunohistochemical analysis.
4% paraformaldehyde-fixed HUVEC (human umbilical vein endothelial cell line) cells stained for CD146 (green) using ab228540 at 1/500 dilution in ICC/IF.
Nuclear counter stain: Hoechst 33342 (blue).
7.5% SDS-PAGE gel.
All lanes: Western blot - Anti-CD146 antibody - C-terminal (ab228540) at 1/1000 dilution
All lanes: Rat liver extract at 50 µg
Predicted band size: 72 kDa
5% SDS-PAGE gel.
All lanes: Western blot - Anti-CD146 antibody - C-terminal (ab228540) at 1/1000 dilution
All lanes: HCT 116 (human colorectal carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 72 kDa
Paraffin-embedded mouse skin tissue stained for CD146 using ab228540 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded mouse colon tissue stained for CD146 using ab228540 at 1/500 dilution in immunohistochemical analysis.
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