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AB228540

Anti-CD146 antibody - C-terminal

4

(2 Reviews)

|

(4 Publications)

Rabbit Polyclonal CD146 antibody. C-terminal. Suitable for WB, IHC-P, ICC/IF and reacts with Rat, Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MCAM.

View Alternative Names

CD146, MUC18, MCAM, Cell surface glycoprotein MUC18, Cell surface glycoprotein P1H12, Melanoma cell adhesion molecule, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, S-endo 1 endothelial-associated antigen

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody - C-terminal (AB228540)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody - C-terminal (AB228540)

Paraffin-embedded J5 xenograft stained for CD146 using ab228540 at 1/500 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-CD146 antibody - C-terminal (AB228540)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD146 antibody - C-terminal (AB228540)

4% paraformaldehyde-fixed HUVEC (human umbilical vein endothelial cell line) cells stained for CD146 (green) using ab228540 at 1/500 dilution in ICC/IF.

Nuclear counter stain : Hoechst 33342 (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody - C-terminal (AB228540)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody - C-terminal (AB228540)

Paraffin-embedded mouse skin tissue stained for CD146 using ab228540 at 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody - C-terminal (AB228540)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody - C-terminal (AB228540)

Paraffin-embedded mouse colon tissue stained for CD146 using ab228540 at 1/500 dilution in immunohistochemical analysis.

Western blot - Anti-CD146 antibody - C-terminal (AB228540)
  • WB

Supplier Data

Western blot - Anti-CD146 antibody - C-terminal (AB228540)

5% SDS-PAGE gel.

All lanes:

Western blot - Anti-CD146 antibody - C-terminal (ab228540) at 1/1000 dilution

All lanes:

HCT 116 (human colorectal carcinoma cell line) whole cell lysate at 30 µg

Predicted band size: 72 kDa

false

Western blot - Anti-CD146 antibody - C-terminal (AB228540)
  • WB

Supplier Data

Western blot - Anti-CD146 antibody - C-terminal (AB228540)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-CD146 antibody - C-terminal (ab228540) at 1/1000 dilution

All lanes:

Rat liver extract at 50 µg

Predicted band size: 72 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human MCAM. The exact immunogen used to generate this antibody is proprietary information.

P43121

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD146 also known as MCAM or MUC18 is a cell adhesion molecule that belongs to the immunoglobulin superfamily. With a mass of approximately 113 kDa CD146 is expressed mainly on the surface of endothelial cells but it can also be found on smooth muscle cells some T-cells and in certain cancerous tissues. This protein plays a role in cell-cell adhesion contributing to the integrity and signaling functions within tissue microenvironments.
Biological function summary

CD146 influences the migration and organization of cells within the vascular system. It serves as a component of cell adhesion complexes facilitating interactions between endothelial cells and other cell types. Additionally CD146 contributes to angiogenesis the process by which new blood vessels form from existing vasculature which is critical during tissue development and repair. This function makes it an important element in maintaining normal physiological processes.

Pathways

CD146 participates in the Wnt/β-catenin and MAPK signaling pathways which are vital in cell proliferation and differentiation. Through the Wnt/β-catenin pathway CD146 interacts with other proteins like Frizzled receptors to regulate gene transcription. In the MAPK pathway it is linked with signaling cascades that involve proteins such as ERK leading to cellular responses necessary for growth and response to external stimuli.

CD146 has been implicated in melanoma progression and cardiovascular diseases. Its overexpression in melanoma associates it with increased tumor growth and metastasis often involving interactions with other adhesion molecules like integrins. In cardiovascular diseases CD146 relates to atherosclerosis as its expression on endothelial cells can promote inflammatory responses. Understanding these connections provides insights into potential therapeutic targets for treating these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as a surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.
See full target information MCAM
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Signal transduction and targeted therapy 10:280 PubMed40887473

2025

Focal adhesion kinase/Src family kinase axis-mediated tyrosine phosphorylation of metabolic enzymes facilitates tumor metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Chen,Jing Zhang,Yuheng Zhu,Yanmeng Zhu,Jingyuan Pang,Qingnan Wu,Yan Wang,Qimin Zhan

Science advances 11:eadw9952 PubMed40864703

2025

Mitochondrial ROS drive foam cell formation via STAT5 signaling in atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Boccuni,Frieda Marka,Manuel Salzmann,Alessia Schirripa,Elisabeth Ableitner,Magdalena Siller,Mira Brekalo,Patrick Haider,Stefan Stojkovic,Christoph Neumayer,Tiit Örd,Karoline Kollmann,Alice Assinger,Thomas Decker,Thomas Köcher,Michael B Fischer,Marion Mußbacher,Andreas Bergthaler,Christian Hengstenberg,Bruno K Podesser,Minna U Kaikkonen,Johann Wojta,Philipp J Hohensinner

Stem cell research & therapy 15:75 PubMed38475906

2024

Menstrual blood-derived mesenchymal stem cells combined with collagen I gel as a regenerative therapeutic strategy for degenerated disc after discectomy in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Li Yu,Honghao Wu,Shumei Zeng,Xiaojian Hu,Yuxu Wu,Jinhong Zhou,Li Yuan,Qingqing Zhang,Charlie Xiang,Zhiyun Feng

Frontiers in physiology 11:572605 PubMed33551826

2021

Long Non-coding RNA MIAT Knockdown Prevents the Formation of Intracranial Aneurysm by Downregulating ENC1 via MYC.

Applications

Unspecified application

Species

Unspecified reactive species

Xinguo Li,Hang Zhao,Jihui Liu,Jing Tong
View all publications
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Product promise

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