AB75769

Anti-CD146 antibody [EPR3208]

Name: Anti-CD146 antibody [EPR3208] KO tested (ab75769) | Abcam
Brand: Abcam Limited
SKU: AB75769
Price: 360 USD
Availability: InStock
Rating: 4 (41 reviews)

RabMAb
Recombinant
KO Validated
What is this?

(41 Reviews)

(186 Publications)

Anti-CD146 antibody [EPR3208] (ab75769) is a rabbit monoclonal antibody detecting CD146 in Western Blot, Flow Cytometry (Intra), IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.

- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency
- Over 140 publications
- Trusted since 2009

View Alternative Names

CD146, MUC18, MCAM, Cell surface glycoprotein MUC18, Cell surface glycoprotein P1H12, Melanoma cell adhesion molecule, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, S-endo 1 endothelial-associated antigen

19 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [EPR3208] (AB75769)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [EPR3208] (AB75769)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of glioma vessels tissue labelling CD146 with unpurified ab75769.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-CD146 antibody [EPR3208] (AB75769)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-CD146 antibody [EPR3208] (AB75769)

Intracellular Flow Cytometry analysis ofHUVEC cells labelling CD146 with purified ab75769 at 1/50 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-CD146 antibody [EPR3208] (AB75769)

Intracellular Flow Cytometry analysis of A375 (human malignant melanoma) cells labeling CD146 with unpurified ab75769 at 1/20 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr^®488) at 1/2000 dilution was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [EPR3208] (AB75769)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling CD146 with purified ab75769 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [EPR3208] (AB75769)

Immunohistochemical analysis of formalin fixed paraffin embedded human tonsil labelling CD146 with ab75769 at a concentration of 0.1µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH8.5 for 32mins. ab75769 Anti-CD146 antibody [EPR3208] was incubated for 16mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual).

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [EPR3208] (AB75769)

Immunohistochemical analysis of formalin fixed paraffin embedded human liver hepatocellular carcinoma labelling CD146 with ab75769 at a concentration of 0.1µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH8.5 for 32mins. ab75769 Anti-CD146 antibody [EPR3208] was incubated for 16mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual).

IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [EPR3208] (AB75769)

Immunohistochemistry experiments were used to compare symptomatic carotid plaques (SC) and asymptomatic carotid plaques (AsC)

Asymptomatic lesions presented higher CD146⁺ pericyte infiltration, p<0.001. Representative images are on the left with corresponding quantification on the right.

ab75769 used at 1/200 dilution.

(After Figure 2 of Davaine et al)

Image from Davaine J.M. et al. PLoS One. 2014 Sep 26;9(9):e107642. doi: 10.1371/journal.pone.0107642. eCollection 2014.

Unknown

Western blot - Anti-CD146 antibody [EPR3208] (AB75769)

Blocking buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-CD146 antibody [EPR3208] (ab75769) at 1/10000 dilution

All lanes:

HUVEC cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/1000 dilution

Predicted band size: 72 kDa

false

Unknown

Western blot - Anti-CD146 antibody [EPR3208] (AB75769)

Blocking buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-CD146 antibody [EPR3208] (ab75769) at 1/10000 dilution

Lane 1:

A375 cell lysate at 20 µg

Lane 2:

Human fetal artery lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/1000 dilution

Predicted band size: 72 kDa

false

Lab

Western blot - Anti-CD146 antibody [EPR3208] (AB75769)

Lane 1 : Wild-type HAP1 whole cell lysate (40 μg)
Lane 2 : CD146 knockout HAP1 whole cell lysate (40 μg)
Lane 3 : A375 whole cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab75769 observed at 120-72 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab75769 was shown to specifically react with CD146 in wild-type HAP1 cells as signal was lost in CD146 knockout cells. Wild-type and CD146 knockout samples were subjected to SDS-PAGE. ab75769 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-CD146 antibody [EPR3208] (ab75769)

Predicted band size: 72 kDa

false

Lab

Western blot - Anti-CD146 antibody [EPR3208] (AB75769)

Lanes 1- 2 : Merged signal (red and green). Green - ab75769 observed at 120 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

ab75769 was shown to react with CD146 in wild-type HeLa cells in western blot. The band observed in CRISPR/Cas9 edited cell line ab261790 (CRISPR/Cas9 edited cell lysate ab256985) lane below 120kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HeLa and MCAM CRISPR/Cas9 edited HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab75769 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-CD146 antibody [EPR3208] (ab75769) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

MCAM CRISPR/Cas9 edited HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human MCAM (CD146) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-mcam-cd146-knockout-hela-cell-line-ab261790'>ab261790</a>)

Predicted band size: 72 kDa

Observed band size: 120 kDa

false

Lab

Western blot - Anti-CD146 antibody [EPR3208] (AB75769)

Lanes 1- 2 : Merged signal (red and green). Green - ab75769 observed at 120 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

ab75769 was shown to react with CD146 in wild-type HeLa cells in western blot. The band observed in knockout cell line ab261790 (knockout cell lysate ab256985) lane below 120kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HeLa and MCAM knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab75769 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-CD146 antibody [EPR3208] (ab75769) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

MCAM knockout HeLa cell lysate at 20 µg

Predicted band size: 72 kDa

Observed band size: 120 kDa

false

Unknown

Western blot - Anti-CD146 antibody [EPR3208] (AB75769)

Blocking buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-CD146 antibody [EPR3208] (ab75769) at 1/10000 dilution

All lanes:

Rat placenta lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/1000 dilution

Predicted band size: 72 kDa

false

Unknown

Western blot - Anti-CD146 antibody [EPR3208] (AB75769)

Blocking buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-CD146 antibody [EPR3208] (ab75769) at 1/10000 dilution

All lanes:

B16-F0 cell lysate at 20 µg/mL

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/1000 dilution

Predicted band size: 72 kDa

false

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [EPR3208] (AB75769)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of urinary bladder transitional carcinoma vessels tissue labelling CD146 with unpurified ab75769.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [EPR3208] (AB75769)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal spleen tissue labelling CD146 with unpurified ab75769.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [EPR3208] (AB75769)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of breast carcinoma vessels tissue labelling CD146 with unpurified ab75769.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [EPR3208] (AB75769)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of melanoma tissue labelling CD146 with unpurified ab75769 at 1/250. A HRP/AP polymerized secondary antibody was used.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [EPR3208] (AB75769)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal tonsil tissue labelling CD146 with unpurified ab75769.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Carrier free

Anti-CD146 antibody [EPR3208] - BSA and Azide free
675 PerCP

PerCP Anti-CD146 antibody [EPR3208]
Biotin

Biotin Anti-CD146 antibody [EPR3208]
578 PE

PE Anti-CD146 antibody [EPR3208]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-CD146 antibody [EPR3208]
660 APC

APC Anti-CD146 antibody [EPR3208]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-CD146 antibody [EPR3208]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-CD146 antibody [EPR3208]
HRP

HRP Anti-CD146 antibody [EPR3208]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-CD146 antibody [EPR3208]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3208

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB, IHC-P, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "For unpurified use at 1/10000 - 1/50000.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/20 - 1/80", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "For unpurified use at 1/10000 - 1/50000.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "For unpurified use at 1/10000 - 1/50000.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Product details

Anti-CD146 antibody [EPR3208] (ab75769) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P, WB in human, mouse, rat samples.

Anti-CD146 antibody [EPR3208] (ab75769) has been cited over 149 times in peer reviewed journals and is trusted by the scientific community.

Abcam's high quality manufacturing and validation processes ensure Anti-CD146 antibody [EPR3208] (ab75769) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.

The specificity of Anti-CD146 antibody [EPR3208] (ab75769) has been confirmed by testing in knockout samples.

Anti-CD146 antibody [EPR3208] (ab75769) has 40 independent reviews from customers.

Anti-CD146 antibody [EPR3208] (ab75769) specifically detects CD146 (UniProt ID: P43121; Molecular weight: 69kDa) and is sold in 100 µL and 1 mL selling sizes.

Conjugation-ready, carrier free format available for antibody clone EPR3208 - ab210072.

Antibody clone EPR3208 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, PE, PerCP, Biotin, APC, HRP, Alexa Fluor® 647, Alexa Fluor® 555 (ab196448, ab209298, ab216361, ab217072, ab303023, ab303024, ab305213, ab307390).

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD146 also known as MCAM or MUC18 is a cell adhesion molecule that belongs to the immunoglobulin superfamily. With a mass of approximately 113 kDa CD146 is expressed mainly on the surface of endothelial cells but it can also be found on smooth muscle cells some T-cells and in certain cancerous tissues. This protein plays a role in cell-cell adhesion contributing to the integrity and signaling functions within tissue microenvironments.

Biological function summary

CD146 influences the migration and organization of cells within the vascular system. It serves as a component of cell adhesion complexes facilitating interactions between endothelial cells and other cell types. Additionally CD146 contributes to angiogenesis the process by which new blood vessels form from existing vasculature which is critical during tissue development and repair. This function makes it an important element in maintaining normal physiological processes.

Pathways

CD146 participates in the Wnt/β-catenin and MAPK signaling pathways which are vital in cell proliferation and differentiation. Through the Wnt/β-catenin pathway CD146 interacts with other proteins like Frizzled receptors to regulate gene transcription. In the MAPK pathway it is linked with signaling cascades that involve proteins such as ERK leading to cellular responses necessary for growth and response to external stimuli.

CD146 has been implicated in melanoma progression and cardiovascular diseases. Its overexpression in melanoma associates it with increased tumor growth and metastasis often involving interactions with other adhesion molecules like integrins. In cardiovascular diseases CD146 relates to atherosclerosis as its expression on endothelial cells can promote inflammatory responses. Understanding these connections provides insights into potential therapeutic targets for treating these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as a surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.

See full target information MCAM

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Publications (186)

Recent publications for all applications. Explore the full list and refine your search

Signal transduction and targeted therapy 10:331 PubMed41052971

2025

Rapid amyloid-β clearance and cognitive recovery through multivalent modulation of blood-brain barrier transport.

Applications

Unspecified application

Species

Unspecified reactive species

Junyang Chen,Pan Xiang,Aroa Duro-Castano,Huawei Cai,Bin Guo,Xiqin Liu,Yifan Yu,Su Lui,Kui Luo,Bowen Ke,Lorena Ruiz-Pérez,Qiyong Gong,Xiaohe Tian,Giuseppe Battaglia

Biochemistry and biophysics reports 44:102221 PubMed40949421

2025

A modified protocol for the isolation and culture of human umbilical vein endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Anmol Sandhu,Anannya Parvathi,Jennifer Jane McGhee,Salim Ismail,I-Ping Loh,Bert van der Werf,Jie Zhang,Trevor Sherwin

Journal of inflammation research 18:11055-11071 PubMed40831515

2025

Single-Cell RNA Sequencing Reveals Pericytes Acquire a Fibrotic Phenotype and Promote Mesenteric Adipose Tissue Fibrosis in Crohn's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Da Zhang,Anqi Yu,Mengjia He,Qian Zhou,Shibo Sun,Lan Bai,Fang Xie

Journal of nanobiotechnology 23:547 PubMed40722154

2025

A cell-free SHED lysate-hydrogel system for oral ulcer healing with anti-inflammatory and pro-angiogenic effects.

Applications

Unspecified application

Species

Unspecified reactive species

Na Dong,Chen Zhang,Qiao Zhang,Sa Bao,Yunfan Hu,Haichao Xu,Peng Dai,Caiyan Li,Shengcun Li,Ping Wu,Junpeng Xu,Xiaojun Cai,Zhouguang Wang,Lihua Luo

Nature communications 16:5448 PubMed40593535

2025

Epitenon-derived progenitors drive fibrosis and regeneration after flexor tendon injury in a spatially-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Anne E C Nichols,Lauren Benoodt,Emmanuella Adjei-Sowah,Kyle Jerreld,Alexander Kollar,Constantinos Ketonis,Alayna E Loiselle

Cancers 17: PubMed40563628

2025

Inhibition of PCSK9 Attenuates Liver Endothelial Cell Activation Induced by Colorectal Cancer Stem Cells During Liver Metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Ander Martin,Daniela Gerovska,Marcos J Arauzo-Bravo,Maitane Duarte García-Escudero,Helena García García,Iratxe Bañares,Naroa Fontal,Geraldine Siegfried,Serge Evrad,Simon Pernot,Abdel-Majid Khatib,Iker Badiola

NPJ precision oncology 9:104 PubMed40221534

2025

Single-cell and spatial transcriptomics reveals the key role of MCAM tip-like endothelial cells in osteosarcoma metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Haijun Tang,Yangjie Cai,Mingxiu Yang,Shengping Tang,Qian Huang,Hening Li,Shangyu Liu,Hongcai Teng,Tianyu Xie,Maolin He,Yun Liu,Shijie Liao

Cell death & disease 16:150 PubMed40032820

2025

CD146 regulates the stemness and chemoresistance of hepatocellular carcinoma via JAG2-NOTCH signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Bing Yan,QiuYu Lu,TianMing Gao,KunQing Xiao,QianNi Zong,HongWei Lv,GuiShuai Lv,Liang Wang,ChunYing Liu,Wen Yang,GuoQing Jiang

Hepatology communications 8: PubMed39992088

2025

MCAM is a prognostic biomarker in patients with liver cirrhosis and HCC.

Applications

Unspecified application

Species

Unspecified reactive species

Eva Stockinger,Hendrik Luxenburger,Dominik Bettinger,Christopher Berlin,David Obwegs, Sagar,Lukas Sturm,Mikhail Gromak,Simon Johannes Gairing,Friedrich Foerster,Christian Labenz,Sabine MacNelly,Tobias Boettler,Philipp Holzner,Peter Bronsert,Bertram Bengsch,Robert Thimme,Maike Hofmann,Natascha Roehlen

Stem cell research & therapy 16:70 PubMed39940043

2025

Pericyte-derived extracellular vesicles improve vascular barrier function in sepsis via the Angpt1/PI3K/AKT pathway and pericyte recruitment: an in vivo and in vitro study.

Applications

Unspecified application

Species

Unspecified reactive species

Zi-Sen Zhang,Ao Yang,Xi Luo,He-Nan Zhou,Yi-Yan Liu,Dai-Qin Bao,Jie Zhang,Jia-Tao Zang,Qing-Hui Li,Tao Li,Liang-Ming Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD146 antibody [EPR3208]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [EPR3208] (AB75769)

Flow Cytometry (Intracellular) - Anti-CD146 antibody [EPR3208] (AB75769)

Flow Cytometry (Intracellular) - Anti-CD146 antibody [EPR3208] (AB75769)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [EPR3208] (AB75769)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [EPR3208] (AB75769)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [EPR3208] (AB75769)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [EPR3208] (AB75769)

Western blot - Anti-CD146 antibody [EPR3208] (AB75769)

Western blot - Anti-CD146 antibody [EPR3208] (AB75769)

Western blot - Anti-CD146 antibody [EPR3208] (AB75769)

Western blot - Anti-CD146 antibody [EPR3208] (AB75769)

Western blot - Anti-CD146 antibody [EPR3208] (AB75769)

Western blot - Anti-CD146 antibody [EPR3208] (AB75769)

Western blot - Anti-CD146 antibody [EPR3208] (AB75769)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [EPR3208] (AB75769)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [EPR3208] (AB75769)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [EPR3208] (AB75769)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [EPR3208] (AB75769)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [EPR3208] (AB75769)

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Key facts

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Carrier free

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Immunogen

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Primary Antibodies

Primary Antibodies

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Reactivity data

Product details

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Appropriate short-term storage duration

Appropriate short-term storage conditions

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