Rabbit Recombinant Monoclonal CD146 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 11 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [EPR3208] - BSA and Azide free (AB210072), expandable thumbnail](https://content.abcam.com/products/images/cd146-antibody-epr3208-bsa-and-azide-free-ab210072--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img45430.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [EPR3208] - BSA and Azide free (AB210072)")
![Western blot - Anti-CD146 antibody [EPR3208] - BSA and Azide free (AB210072), expandable thumbnail](https://content.abcam.com/products/images/cd146-antibody-epr3208-bsa-and-azide-free-ab210072--western-blot-img139747.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CD146 antibody [EPR3208] - BSA and Azide free (AB210072)")
![Flow Cytometry (Intracellular) - Anti-CD146 antibody [EPR3208] - BSA and Azide free (AB210072), expandable thumbnail](https://content.abcam.com/products/images/cd146-antibody-epr3208-bsa-and-azide-free-ab210072--flow-cytometry-intracellular-img140541.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-CD146 antibody [EPR3208] - BSA and Azide free (AB210072)")
![Western blot - Anti-CD146 antibody [EPR3208] - BSA and Azide free (AB210072), expandable thumbnail](https://content.abcam.com/products/images/cd146-antibody-epr3208-bsa-and-azide-free-ab210072--western-blot-img158098.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CD146 antibody [EPR3208] - BSA and Azide free (AB210072)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [EPR3208] - BSA and Azide free (AB210072), expandable thumbnail](https://content.abcam.com/products/images/cd146-antibody-epr3208-bsa-and-azide-free-ab210072--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135956.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [EPR3208] - BSA and Azide free (AB210072)")
Publications
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- PloS one 9:e1074372014Targeting apoptosis signalling kinase-1 (ASK-1) does not prevent the development of neuropathy in streptozotocin-induced diabetic mice.
- Nature 514:585-902014Mesenchymal-endothelial transition contributes to cardiac neovascularization.
- PloS one 9:e1076422014Osteoprotegerin, pericytes and bone-like vascular calcification are associated with carotid plaque stability.
- Biomaterials 35:9660-72014Bone tissue formation with human mesenchymal stem cells and biphasic calcium phosphate ceramics: the local implication of osteoclasts and macrophages.
- Proceedings of the National Academy of Sciences of 111:E1924-322014Fetal programming of adult Leydig cell function by androgenic effects on stem/progenitor cells.Applications:IHC-PReactive species:Unspecified reactive speciesKaren R Kilcoyne et. al.PubMed 24753613
- Journal of dental research 91:58-642011Bmp2 is required for odontoblast differentiation and pulp vasculogenesis.Applications:Unspecified applicationReactive species:MouseW Yang,M A Harris,Y Cui,Y Mishina,S E Harris,J Gluhak-HeinrichPubMed 21984706
- Tissue engineering. Part A 17:2123-322011Characterization of urine-derived stem cells obtained from upper urinary tract for use in cell-based urological tissue engineering.Applications:ICC/IFReactive species:HumanShantaram Bharadwaj,Guihua Liu,Yingai Shi,Chad Markert,Karl-Erik Andersson,Anthony Atala,Yuanyuan ZhangPubMed 21513463
- Cancer science 102:648-552011Establishment of a cell line from a Japanese patient useful for generating an in vivo model of malignant pleural mesothelioma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAyuko Sato et. al.PubMed 21205078
- Biomaterials 31:8889-9012010Tissue-engineered conduit using urine-derived stem cells seeded bacterial cellulose polymer in urinary reconstruction and diversion.
- Modern pathology : an official journal of the Unit 23:1458-662010Immunocytochemistry of CD146 is useful to discriminate between malignant pleural mesothelioma and reactive mesothelium.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected | Expected |
| Rat | Expected | Tested | Expected | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
11 products for Alternative Product
9 products for Alternative Version
Target data
Function
Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.
Alternative names
Cell surface glycoprotein MUC18, Cell surface glycoprotein P1H12, Melanoma cell adhesion molecule, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, S-endo 1 endothelial-associated antigen, MCAM, MUC18
Recommended products
Rabbit Recombinant Monoclonal CD146 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 11 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR3208
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab210072 is the carrier-free version of Anti-CD146 antibody [EPR3208] ab75769.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD146 also known as MCAM or MUC18 is a cell adhesion molecule that belongs to the immunoglobulin superfamily. With a mass of approximately 113 kDa CD146 is expressed mainly on the surface of endothelial cells but it can also be found on smooth muscle cells some T-cells and in certain cancerous tissues. This protein plays a role in cell-cell adhesion contributing to the integrity and signaling functions within tissue microenvironments.
- Biological function summary
CD146 influences the migration and organization of cells within the vascular system. It serves as a component of cell adhesion complexes facilitating interactions between endothelial cells and other cell types. Additionally CD146 contributes to angiogenesis the process by which new blood vessels form from existing vasculature which is critical during tissue development and repair. This function makes it an important element in maintaining normal physiological processes.
- Pathways
CD146 participates in the Wnt/β-catenin and MAPK signaling pathways which are vital in cell proliferation and differentiation. Through the Wnt/β-catenin pathway CD146 interacts with other proteins like Frizzled receptors to regulate gene transcription. In the MAPK pathway it is linked with signaling cascades that involve proteins such as ERK leading to cellular responses necessary for growth and response to external stimuli.
- Associated diseases and disorders
CD146 has been implicated in melanoma progression and cardiovascular diseases. Its overexpression in melanoma associates it with increased tumor growth and metastasis often involving interactions with other adhesion molecules like integrins. In cardiovascular diseases CD146 relates to atherosclerosis as its expression on endothelial cells can promote inflammatory responses. Understanding these connections provides insights into potential therapeutic targets for treating these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
12 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [EPR3208] - BSA and Azide free (ab210072), expandable thumbnail](https://content.abcam.com/products/images/cd146-antibody-epr3208-bsa-and-azide-free-ab210072--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img45430.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [EPR3208] - BSA and Azide free (ab210072)")
Image from Davaine J.M. et al. PLoS One. 2014 Sep 26;9(9):e107642. doi: 10.1371/journal.pone.0107642. eCollection 2014.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [EPR3208] - BSA and Azide free (ab210072)
Immunohistochemistry experiments were used to compare symptomatic carotid plaques (SC) and asymptomatic carotid plaques (AsC)
Asymptomatic lesions presented higher CD146+ pericyte infiltration, p
Anti-CD146 antibody [EPR3208] ab75769 used at 1/200 dilution.
(After Figure 2 of Davaine et al)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD146 antibody [EPR3208] ab75769).
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
![Western blot - Anti-CD146 antibody [EPR3208] - BSA and Azide free (ab210072), expandable thumbnail](https://content.abcam.com/products/images/cd146-antibody-epr3208-bsa-and-azide-free-ab210072--western-blot-img139747.jpg "Western blot - Anti-CD146 antibody [EPR3208] - BSA and Azide free (ab210072)")
Western blot - Anti-CD146 antibody [EPR3208] - BSA and Azide free (ab210072)
Lane 1: Wild-type HAP1 whole cell lysate (40 μg)
Lane 2: CD146 knockout HAP1 whole cell lysate (40 μg)
Lane 3: A375 whole cell lysate (20 μg)Lanes 1 - 4: Merged signal (red and green). Green - Anti-CD146 antibody [EPR3208] ab75769 observed at 120-72 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.
Anti-CD146 antibody [EPR3208] ab75769 was shown to specifically react with CD146 in wild-type HAP1 cells as signal was lost in CD146 knockout cells. Wild-type and CD146 knockout samples were subjected to SDS-PAGE. Anti-CD146 antibody [EPR3208] ab75769 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD146 antibody [EPR3208] ab75769).
All lanes: Western blot - Anti-CD146 antibody [EPR3208] - BSA and Azide free (ab210072)
Predicted band size: 72 kDa
![Flow Cytometry (Intracellular) - Anti-CD146 antibody [EPR3208] - BSA and Azide free (ab210072), expandable thumbnail](https://content.abcam.com/products/images/cd146-antibody-epr3208-bsa-and-azide-free-ab210072--flow-cytometry-intracellular-img140541.jpg "Flow Cytometry (Intracellular) - Anti-CD146 antibody [EPR3208] - BSA and Azide free (ab210072)")
Flow Cytometry (Intracellular) - Anti-CD146 antibody [EPR3208] - BSA and Azide free (ab210072)
Intracellular Flow Cytometry analysis of A375 (human malignant melanoma) cells labeling CD146 with unpurified Anti-CD146 antibody [EPR3208] ab75769 at 1/20 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor®488) at 1/2000 dilution was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD146 antibody [EPR3208] ab75769).
![Western blot - Anti-CD146 antibody [EPR3208] - BSA and Azide free (ab210072), expandable thumbnail](https://content.abcam.com/products/images/cd146-antibody-epr3208-bsa-and-azide-free-ab210072--western-blot-img158098.jpg "Western blot - Anti-CD146 antibody [EPR3208] - BSA and Azide free (ab210072)")
Western blot - Anti-CD146 antibody [EPR3208] - BSA and Azide free (ab210072)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD146 antibody [EPR3208] ab75769).
Lanes 1- 2: Merged signal (red and green). Green - Anti-CD146 antibody [EPR3208] ab75769 observed at 120 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.
Anti-CD146 antibody [EPR3208] ab75769 was shown to react with CD146 in wild-type HeLa cells in western blot. The band observed in CRISPR/Cas9 edited cell line Human MCAM (CD146) knockout HeLa cell line ab261790 (CRISPR/Cas9 edited cell lysate Human MCAM (CD146) knockout HeLa cell lysate ab256985) lane below 120kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HeLa and MCAM CRISPR/Cas9 edited HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. Anti-CD146 antibody [EPR3208] ab75769 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-CD146 antibody [EPR3208] (Anti-CD146 antibody [EPR3208] ab75769) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: MCAM CRISPR/Cas9 edited HeLa cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 72 kDa
Observed band size: 120 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [EPR3208] - BSA and Azide free (ab210072), expandable thumbnail](https://content.abcam.com/products/images/cd146-antibody-epr3208-bsa-and-azide-free-ab210072--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135956.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [EPR3208] - BSA and Azide free (ab210072)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [EPR3208] - BSA and Azide free (ab210072)
This IHC data was generated using the same anti-CD146 antibody clone, EPR3208, in a different buffer formulation (cat# Anti-CD146 antibody [EPR3208] ab75769).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling CD146 with purified Anti-CD146 antibody [EPR3208] ab75769 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
![Flow Cytometry (Intracellular) - Anti-CD146 antibody [EPR3208] - BSA and Azide free (ab210072), expandable thumbnail](https://content.abcam.com/products/images/cd146-antibody-epr3208-bsa-and-azide-free-ab210072--flow-cytometry-intracellular-img106237.jpg "Flow Cytometry (Intracellular) - Anti-CD146 antibody [EPR3208] - BSA and Azide free (ab210072)")
Flow Cytometry (Intracellular) - Anti-CD146 antibody [EPR3208] - BSA and Azide free (ab210072)
Intracellular Flow Cytometry analysis of HUVEC cells labelling CD146 with purified Anti-CD146 antibody [EPR3208] ab75769 at 1/50 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD146 antibody [EPR3208] ab75769).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [EPR3208] - BSA and Azide free (ab210072), expandable thumbnail](https://content.abcam.com/products/images/cd146-antibody-epr3208-bsa-and-azide-free-ab210072--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img30310.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [EPR3208] - BSA and Azide free (ab210072)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [EPR3208] - BSA and Azide free (ab210072)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of melanoma tissue labelling CD146 with unpurified Anti-CD146 antibody [EPR3208] ab75769 at 1/250. A HRP/AP polymerized secondary antibody was used.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD146 antibody [EPR3208] ab75769).
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [EPR3208] - BSA and Azide free (ab210072), expandable thumbnail](https://content.abcam.com/products/images/cd146-antibody-epr3208-bsa-and-azide-free-ab210072--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img105832.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [EPR3208] - BSA and Azide free (ab210072)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [EPR3208] - BSA and Azide free (ab210072)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of breast carcinoma vessels tissue labelling CD146 with unpurified Anti-CD146 antibody [EPR3208] ab75769.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD146 antibody [EPR3208] ab75769).
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [EPR3208] - BSA and Azide free (ab210072), expandable thumbnail](https://content.abcam.com/products/images/cd146-antibody-epr3208-bsa-and-azide-free-ab210072--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img123073.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [EPR3208] - BSA and Azide free (ab210072)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [EPR3208] - BSA and Azide free (ab210072)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of urinary bladder transitional carcinoma vessels tissue labelling CD146 with unpurified Anti-CD146 antibody [EPR3208] ab75769.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD146 antibody [EPR3208] ab75769).
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [EPR3208] - BSA and Azide free (ab210072), expandable thumbnail](https://content.abcam.com/products/images/cd146-antibody-epr3208-bsa-and-azide-free-ab210072--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133634.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [EPR3208] - BSA and Azide free (ab210072)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [EPR3208] - BSA and Azide free (ab210072)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of glioma vessels tissue labelling CD146 with unpurified Anti-CD146 antibody [EPR3208] ab75769.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD146 antibody [EPR3208] ab75769).
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [EPR3208] - BSA and Azide free (ab210072), expandable thumbnail](https://content.abcam.com/products/images/cd146-antibody-epr3208-bsa-and-azide-free-ab210072--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img128862.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [EPR3208] - BSA and Azide free (ab210072)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [EPR3208] - BSA and Azide free (ab210072)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal tonsil tissue labelling CD146 with unpurified Anti-CD146 antibody [EPR3208] ab75769.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD146 antibody [EPR3208] ab75769).
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [EPR3208] - BSA and Azide free (ab210072), expandable thumbnail](https://content.abcam.com/products/images/cd146-antibody-epr3208-bsa-and-azide-free-ab210072--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135578.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [EPR3208] - BSA and Azide free (ab210072)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [EPR3208] - BSA and Azide free (ab210072)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal spleen tissue labelling CD146 with unpurified Anti-CD146 antibody [EPR3208] ab75769.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD146 antibody [EPR3208] ab75769).
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-CD146 antibody [EPR3208] - BSA and Azide free (ab210072), expandable thumbnail](https://content.abcam.com/products/images/cd146-antibody-epr3208-bsa-and-azide-free-ab210072--flow-cytometry-intracellular-img106237.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-CD146 antibody [EPR3208] - BSA and Azide free (ab210072)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [EPR3208] - BSA and Azide free (ab210072), expandable thumbnail](https://content.abcam.com/products/images/cd146-antibody-epr3208-bsa-and-azide-free-ab210072--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img30310.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [EPR3208] - BSA and Azide free (ab210072)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [EPR3208] - BSA and Azide free (ab210072), expandable thumbnail](https://content.abcam.com/products/images/cd146-antibody-epr3208-bsa-and-azide-free-ab210072--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img105832.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [EPR3208] - BSA and Azide free (ab210072)")
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