AB233923

Anti-CD146 antibody [MUC18/1130]

Name: Anti-CD146 antibody [MUC18/1130] (ab233923) | Abcam
Brand: Abcam Limited
SKU: AB233923
Price: 525 USD
Availability: InStock

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(5 Publications)

Mouse Monoclonal CD146 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MCAM.

View Alternative Names

CD146, MUC18, MCAM, Cell surface glycoprotein MUC18, Cell surface glycoprotein P1H12, Melanoma cell adhesion molecule, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, S-endo 1 endothelial-associated antigen

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MUC18/1130

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human MCAM.

P43121

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "<p>(Primary incubation for 30 min at RT).</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

Purified from Bioreactor Concentrate by Protein A/G.

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD146 also known as MCAM or MUC18 is a cell adhesion molecule that belongs to the immunoglobulin superfamily. With a mass of approximately 113 kDa CD146 is expressed mainly on the surface of endothelial cells but it can also be found on smooth muscle cells some T-cells and in certain cancerous tissues. This protein plays a role in cell-cell adhesion contributing to the integrity and signaling functions within tissue microenvironments.

Biological function summary

CD146 influences the migration and organization of cells within the vascular system. It serves as a component of cell adhesion complexes facilitating interactions between endothelial cells and other cell types. Additionally CD146 contributes to angiogenesis the process by which new blood vessels form from existing vasculature which is critical during tissue development and repair. This function makes it an important element in maintaining normal physiological processes.

Pathways

CD146 participates in the Wnt/β-catenin and MAPK signaling pathways which are vital in cell proliferation and differentiation. Through the Wnt/β-catenin pathway CD146 interacts with other proteins like Frizzled receptors to regulate gene transcription. In the MAPK pathway it is linked with signaling cascades that involve proteins such as ERK leading to cellular responses necessary for growth and response to external stimuli.

CD146 has been implicated in melanoma progression and cardiovascular diseases. Its overexpression in melanoma associates it with increased tumor growth and metastasis often involving interactions with other adhesion molecules like integrins. In cardiovascular diseases CD146 relates to atherosclerosis as its expression on endothelial cells can promote inflammatory responses. Understanding these connections provides insights into potential therapeutic targets for treating these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as a surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.

See full target information MCAM

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Extracellular vesicle 3: PubMed38872854

2024

A multi-organ, feto-maternal interface organ-on-chip, models pregnancy pathology and is a useful preclinical extracellular vesicle drug trial platform.

Applications

Unspecified application

Species

Unspecified reactive species

Melody Safarzadeh,Lauren S Richardson,Ananth Kumar Kammala,Angela Mosebarger,Mohamed Bettayeb,Sungjin Kim,Po Yi Lam,Enkhtuya Radnaa,Arum Han,Ramkumar Menon

Lab on a chip 24:1727-1749 PubMed38334486

2024

Endocrine-disrupting compounds and their impact on human placental function: evidence from placenta organ-on-chip studies.

Applications

Unspecified application

Species

Unspecified reactive species

Manuel S Vidal,Lauren S Richardson,Ananth Kumar Kammala,Sungjin Kim,Po Yi Lam,Rahul Cherukuri,Tilu Jain Thomas,Mohammed Bettayeb,Arum Han,Ivan Rusyn,Ramkumar Menon

Frontiers in pharmacology 14:1241815 PubMed37663251

2023

Microfluidic technology and simulation models in studying pharmacokinetics during pregnancy.

Applications

Unspecified application

Species

Unspecified reactive species

Ananth K Kammala,Lauren S Richardson,Enkhtuya Radnaa,Arum Han,Ramkumar Menon

Theranostics 11:10064-10073 PubMed34815804

2021

Heterogeneity of cell composition and origin identified by single-cell transcriptomics in renal cysts of patients with autosomal dominant polycystic kidney disease.

Applications

Unspecified application

Species

Unspecified reactive species

Qiong Li,Yuchen Wang,Wenfeng Deng,Yanna Liu,Jian Geng,Ziyan Yan,Fei Li,Binshen Chen,Zhuolin Li,Renfei Xia,Wenli Zeng,Rumin Liu,Jian Xu,Fu Xiong,Chin-Lee Wu,Yun Miao

Journal of experimental & clinical cancer research 39:273 PubMed33278894

2020

Characterization of novel neutralizing mouse monoclonal antibody JM1-24-3 developed against MUC18 in metastatic melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Runhua Feng,Yuling Wang,Vijaya Ramachandran,Qinhong Ma,Matthew M May,Ming Li,Joe X Zhou,Xiang Xu,Kejing Xu,Shenying Fang,Weiya Xia,Dawen Sui,Huey Liu,Xiaolian Gao,Victor Prieto,Stephen C Blacklow,Mason Lu,Jeffrey E Lee

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD146 antibody [MUC18/1130]

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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