Mouse Monoclonal CD147 antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human, Transfected cell lysate samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human BSG.
Images
![Western blot - Anti-CD147 antibody [10E10] (AB230921), expandable thumbnail](https://content.abcam.com/products/images/cd147-antibody-10e10-ab230921--western-blot-img146817.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CD147 antibody [10E10] (AB230921)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD147 antibody [10E10] (AB230921), expandable thumbnail](https://content.abcam.com/products/images/cd147-antibody-10e10-ab230921--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img22712.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD147 antibody [10E10] (AB230921)")
![Western blot - Anti-CD147 antibody [10E10] (AB230921), expandable thumbnail](https://content.abcam.com/products/images/cd147-antibody-10e10-ab230921--western-blot-img2388.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CD147 antibody [10E10] (AB230921)")
![Flow Cytometry (Intracellular) - Anti-CD147 antibody [10E10] (AB230921), expandable thumbnail](https://content.abcam.com/products/images/cd147-antibody-10e10-ab230921--flow-cytometry-intracellular-img51067.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-CD147 antibody [10E10] (AB230921)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD147 antibody [10E10] (AB230921), expandable thumbnail](https://content.abcam.com/products/images/cd147-antibody-10e10-ab230921--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img99611.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD147 antibody [10E10] (AB230921)")
Publications
Filter by
- Experimental hematology & oncology 12:42023Alpha-B-Crystallin overexpression is sufficient to promote tumorigenesis and metastasis in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBehnam Rashidieh,Amanda Louise Bain,Simon Manuel Tria,Sowmya Sharma,Cameron Allan Stewart,Jacinta Ley Simmons,Pirjo M Apaja,Pascal H G Duijf,John Finnie,Kum Kum KhannaPubMed 36624493
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human BSG. Database link P35613
Abcam Recommends
Reactivity data
Select an application| WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Monkey | Predicted | Predicted | Predicted | Predicted |
| Transfected cell lysate | Tested | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/2000 | Notes - |
Species Transfected cell lysate | Dilution info 1/500 - 1/2000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Monkey | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Monkey | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Monkey | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Monkey | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate | Dilution info - | Notes - |
Associated Products
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6 products for Alternative Product
Target data
Function
Isoform 1. Essential for normal retinal maturation and development (By similarity). Acts as a retinal cell surface receptor for NXNL1 and plays an important role in NXNL1-mediated survival of retinal cone photoreceptors (PubMed:25957687). In association with glucose transporter SLC16A1/GLUT1 and NXNL1, promotes retinal cone survival by enhancing aerobic glycolysis and accelerating the entry of glucose into photoreceptors (PubMed:25957687). May act as a potent stimulator of IL6 secretion in multiple cell lines that include monocytes (PubMed:21620857). Isoform 1. (Microbial infection) Erythrocyte receptor for P.falciparum RH5 which is essential for erythrocyte invasion by the merozoite stage of P.falciparum isolates 3D7 and Dd2. Isoform 2. Signaling receptor for cyclophilins, essential for PPIA/CYPA and PPIB/CYPB-dependent signaling related to chemotaxis and adhesion of immune cells (PubMed:11688976, PubMed:11943775). Plays an important role in targeting monocarboxylate transporters SLC16A1/GLUT1, SLC16A11 and SLC16A12 to the plasma membrane (PubMed:17127621, PubMed:21778275, PubMed:28666119). Acts as a coreceptor for vascular endothelial growth factor receptor 2 (KDR/VEGFR2) in endothelial cells enhancing its VEGFA-mediated activation and downstream signaling (PubMed:25825981). Promotes angiogenesis through EPAS1/HIF2A-mediated up-regulation of VEGFA (isoform VEGF-165 and VEGF-121) and KDR/VEGFR2 in endothelial cells (PubMed:19837976). Plays a key role in regulating tumor growth, invasion, metastasis and neoangiogenesis by stimulating the production and release of extracellular matrix metalloproteinases and KDR/VEGFR2 by both tumor cells and stromal cells (fibroblasts and endothelial cells) (PubMed:11992541, PubMed:12553375, PubMed:15833850). Isoform 2. (Microbial infection) Erythrocyte receptor for P.falciparum RH5 which is essential for erythrocyte invasion by the merozoite stage of P.falciparum isolates 3D7, Dd2, 7G8 and HB3 (PubMed:22080952, PubMed:26195724). Binding of P.falciparum RH5 results in BSG dimerization which triggers an increase in intracellular Ca(2+) in the erythrocyte (PubMed:28409866). This essential step leads to a rearrangement of the erythrocyte cytoskeleton required for the merozoite invasion (PubMed:28409866). Isoform 2. (Microbial infection) Can facilitate human SARS coronavirus (SARS-CoV-1) infection via its interaction with virus-associated PPIA/CYPA. Isoform 2. (Microbial infection) Can facilitate HIV-1 infection via its interaction with virus-associated PPIA/CYPA. Isoform 2. (Microbial infection) First described as a receptor for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), it is not required for SARS-CoV-2 infection. Isoform 2. (Microbial infection) Acts as a receptor for measles virus. Isoform 2. (Microbial infection) Promotes entry of pentamer-expressing human cytomegalovirus (HCMV) into epithelial and endothelial cells.
Alternative names
CD147, UNQ6505/PRO21383, BSG, Basigin, 5F7, Collagenase stimulatory factor, Extracellular matrix metalloproteinase inducer, Hepatoma-associated antigen, Leukocyte activation antigen M6, OK blood group antigen, Tumor cell-derived collagenase stimulatory factor, EMMPRIN, HAb18G, TCSF
Recommended products
Mouse Monoclonal CD147 antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human, Transfected cell lysate samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human BSG.
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human BSG. Database link P35613
- Clone number
- 10E10
- Purification technique
- Affinity purification Protein A/G
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD147 also referred to as EMMPRIN or basigin is a transmembrane glycoprotein with a molecular weight of roughly 50–60 kDa. This protein is found on the surface of many cell types including leukocytes platelets and endothelial cells and it plays a critical role in cell function related to immune responses and cellular interactions. CD147 interacts with several cellular components serving mechanical functions such as facilitating cell-to-cell communication and contributing to the stability of cell structures. The protein is ubiquitously expressed but exhibits higher levels in tissues like the brain skin and liver.
- Biological function summary
CD147 engages in multiple roles beyond its mechanical functions. It functions as a part of protein complexes and is involved in processes such as regulation of matrix metalloproteinases (MMPs) which are pivotal in tissue remodeling and repair. It can modulate inflammatory responses and is essential for cellular signaling pathways that affect cellular metabolism and growth. The involvement of CD147 in immune responses indicates its importance in various physiological processes.
- Pathways
CD147 plays a significant role in the MAPK and NF-kB signaling pathways which are fundamental for controlling inflammatory responses and cell survival. It interacts with proteins such as integrins and cyclophilins which are important for mediating these pathways. These interactions highlight CD147's impact on cellular dynamics and its potential role in modulating the cellular environment in response to external stimuli.
- Associated diseases and disorders
CD147 has been implicated in cancer promotion and progression as well as inflammatory diseases like rheumatoid arthritis. Its overexpression is often observed in tumors where it interacts with MMPs to facilitate tumor invasion and metastasis. In inflammatory conditions CD147 can influence the behavior of proteins like cytokines exacerbating symptoms and contributing to disease severity. These associations make CD147 a potential target for therapeutic intervention in cancer and inflammatory diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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8 product images
![Western blot - Anti-CD147 antibody [10E10] (ab230921), expandable thumbnail](https://content.abcam.com/products/images/cd147-antibody-10e10-ab230921--western-blot-img146817.jpg "Western blot - Anti-CD147 antibody [10E10] (ab230921)")
Western blot - Anti-CD147 antibody [10E10] (ab230921)
All lanes: Western blot - Anti-CD147 antibody [10E10] (ab230921) at 1/500 dilution
Lane 1: pCMV6-ENTRY control-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 5 µg
Lane 2: pCMV6-ENTRY CD147-transfected HEK-293T whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 42 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD147 antibody [10E10] (ab230921), expandable thumbnail](https://content.abcam.com/products/images/cd147-antibody-10e10-ab230921--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img22712.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD147 antibody [10E10] (ab230921)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD147 antibody [10E10] (ab230921)
Formalin-fixed, paraffin-embedded human brain tissue stained for CD147 using ab230921 at 10 μg/ml in immunohistochemical analysis.
![Western blot - Anti-CD147 antibody [10E10] (ab230921), expandable thumbnail](https://content.abcam.com/products/images/cd147-antibody-10e10-ab230921--western-blot-img2388.jpg "Western blot - Anti-CD147 antibody [10E10] (ab230921)")
Western blot - Anti-CD147 antibody [10E10] (ab230921)
Lanes 1 - 4: Merged signal (red and green). Green - ab230921 observed at 42-70 kDa. Red - loading control Anti-alpha Tubulin antibody [EP1332Y] - Loading Control ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55kDa.ab230921 was shown to react with CD147 in wild-type A549 cells in western blot with loss of signal observed in BSG knockout cell line Human BSG (CD147) knockout A549 cell line ab273748 (knockout cell lysate Human BSG (CD147) knockout A549 cell lysate ab275500). Wild-type and BSG knockout A549 cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab230921 and Anti-alpha Tubulin antibody [EP1332Y] - Loading Control ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4°C at a 1 in 500 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-CD147 antibody [10E10] (ab230921) at 1/500 dilution
Lane 1: Wild-type A549 cell lysate at 30 µg
Lane 2: BSG knockout A549 cell lysate at 30 µg
Lane 2: Western blot - Human BSG (CD147) knockout A549 cell line (Human BSG (CD147) knockout A549 cell line ab273748)
Lane 3: Raji cell lysate at 30 µg
Lane 4: Jurkat cell lysate at 30 µg
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 42-70 kDa
![Flow Cytometry (Intracellular) - Anti-CD147 antibody [10E10] (ab230921), expandable thumbnail](https://content.abcam.com/products/images/cd147-antibody-10e10-ab230921--flow-cytometry-intracellular-img51067.jpg "Flow Cytometry (Intracellular) - Anti-CD147 antibody [10E10] (ab230921)")
Flow Cytometry (Intracellular) - Anti-CD147 antibody [10E10] (ab230921)
Intracellular Flow Cytometry overlay histogram showing wild-type A549 (green line) and BSG knockout A549 cells (Human BSG (CD147) knockout A549 cell line ab273748) stained with ab230921 (red line). The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab230921) (1x106 in 100μl at 5 μg/ml) for 30 min at 4°C.
The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor® 488, pre-adsorbed) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117) was used at 1/2000 for 30 min at 4°C. Isotype control antibody was mouse IgG1K (Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control ab170190) used at the same concentration and conditions as the primary antibody (wild-type A549 - black line; BSG knockout A549 - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).
Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD147 antibody [10E10] (ab230921), expandable thumbnail](https://content.abcam.com/products/images/cd147-antibody-10e10-ab230921--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img99611.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD147 antibody [10E10] (ab230921)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD147 antibody [10E10] (ab230921)
Formalin-fixed, paraffin-embedded human placenta tissue stained for CD147 using ab230921 at 10 μg/ml in immunohistochemical analysis.
![Immunocytochemistry/ Immunofluorescence - Anti-CD147 antibody [10E10] (ab230921), expandable thumbnail](https://content.abcam.com/products/images/cd147-antibody-10e10-ab230921--immunocytochemistry-immunofluorescence-img112008.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CD147 antibody [10E10] (ab230921)")
Immunocytochemistry/ Immunofluorescence - Anti-CD147 antibody [10E10] (ab230921)
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for CD147 (green) using ab230921 at 1/100 dilution in ICC/IF.
![Flow Cytometry (Intracellular) - Anti-CD147 antibody [10E10] (ab230921), expandable thumbnail](https://content.abcam.com/products/images/cd147-antibody-10e10-ab230921--flow-cytometry-intracellular-img47742.jpg "Flow Cytometry (Intracellular) - Anti-CD147 antibody [10E10] (ab230921)")
Flow Cytometry (Intracellular) - Anti-CD147 antibody [10E10] (ab230921)
Intracellular flow cytometric analysis ofHeLa (human T cell leukemia cell line from peripheral blood) cell line labeling CD147 with ab230921 at 1/100 dilution (red) compared to a nonspecific negative control antibody (blue).
![Flow Cytometry (Intracellular) - Anti-CD147 antibody [10E10] (ab230921), expandable thumbnail](https://content.abcam.com/products/images/cd147-antibody-10e10-ab230921--flow-cytometry-intracellular-img47749.jpg "Flow Cytometry (Intracellular) - Anti-CD147 antibody [10E10] (ab230921)")
Flow Cytometry (Intracellular) - Anti-CD147 antibody [10E10] (ab230921)
Intracellular flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cell line labeling CD147with ab230921 at 1/100 dilution (red) compared to a nonspecific negative control antibody (blue).
![Immunocytochemistry/ Immunofluorescence - Anti-CD147 antibody [10E10] (ab230921), expandable thumbnail](https://content.abcam.com/products/images/cd147-antibody-10e10-ab230921--immunocytochemistry-immunofluorescence-img112008.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CD147 antibody [10E10] (ab230921)")
![Flow Cytometry (Intracellular) - Anti-CD147 antibody [10E10] (ab230921), expandable thumbnail](https://content.abcam.com/products/images/cd147-antibody-10e10-ab230921--flow-cytometry-intracellular-img47742.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-CD147 antibody [10E10] (ab230921)")
![Flow Cytometry (Intracellular) - Anti-CD147 antibody [10E10] (ab230921), expandable thumbnail](https://content.abcam.com/products/images/cd147-antibody-10e10-ab230921--flow-cytometry-intracellular-img47749.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-CD147 antibody [10E10] (ab230921)")
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