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Rabbit Recombinant Monoclonal CD147 antibody. Carrier free. Suitable for IP, Flow Cyt, WB, sELISA, ICC/IF, IHC-P and reacts with Mouse, Recombinant fragment, Recombinant fragment - Mouse samples.

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Images

Flow Cytometry - Anti-CD147 antibody [EPR18008-8] - BSA and Azide free (AB222389), expandable thumbnail
  • Immunoprecipitation - Anti-CD147 antibody [EPR18008-8] - BSA and Azide free (AB222389), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CD147 antibody [EPR18008-8] - BSA and Azide free (AB222389), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD147 antibody [EPR18008-8] - BSA and Azide free (AB222389), expandable thumbnail
  • Sandwich ELISA - Anti-CD147 antibody [EPR18008-8] - BSA and Azide free (AB222389), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBsELISAICC/IFIHC-P
Mouse
Tested
Tested
Expected
Expected
Tested
Tested
Recombinant fragment
Not recommended
Not recommended
Expected
Not recommended
Not recommended
Not recommended
Recombinant fragment - Mouse
Not recommended
Not recommended
Not recommended
Tested
Not recommended
Not recommended

Tested
Tested

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Recombinant fragment, Recombinant fragment - Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

ab199376 - Rabbit monoclonal IgG, is suitable for use an isotype control with this antibody.

Not recommended
Not recommended

Species
Recombinant fragment, Recombinant fragment - Mouse
Dilution info
-
Notes

-

Expected
Expected

Species
Recombinant fragment, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Recombinant fragment - Mouse
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment, Recombinant fragment - Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Recombinant fragment, Recombinant fragment - Mouse
Dilution info
-
Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

3 products for Alternative Version

Target data

Function

Isoform 1. Essential for normal retinal maturation and development (PubMed:10967074, PubMed:11273674, PubMed:11853760). Acts as a retinal cell surface receptor for NXNL1 and plays an important role in NXNL1-mediated survival of retinal cone photoreceptors (PubMed:25957687). In association with glucose transporter SLC16A1/GLUT1 and NXNL1, promotes retinal cone survival by enhancing aerobic glycolysis and accelerating the entry of glucose into photoreceptors (PubMed:25957687). Isoform 2. Signaling receptor for cyclophilins, essential for PPIA/CYPA and PPIB/CYPB-dependent signaling related to chemotaxis and adhesion of immune cells (By similarity). Plays an important role in targeting the monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to the plasma membrane (PubMed:12601063). Acts as a coreceptor for vascular endothelial growth factor receptor 2 (KDR/VEGFR2) in endothelial cells enhancing its VEGFA-mediated activation and downstream signaling (By similarity). Promotes angiogenesis through EPAS1/HIF2A-mediated up-regulation of VEGFA and KDR/VEGFR2 in endothelial cells (By similarity). Plays an important role in spermatogenesis; mediates interactions between germ cells and Sertoli cell and is essential for the development/differentiation of germ cells to round spermatids (PubMed:11882021, PubMed:23727514).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal CD147 antibody. Carrier free. Suitable for IP, Flow Cyt, WB, sELISA, ICC/IF, IHC-P and reacts with Mouse, Recombinant fragment, Recombinant fragment - Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR18008-8
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab222389 is the carrier-free version of Anti-CD147 antibody [EPR18008-8] ab188190.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CD147 also referred to as EMMPRIN or basigin is a transmembrane glycoprotein with a molecular weight of roughly 50–60 kDa. This protein is found on the surface of many cell types including leukocytes platelets and endothelial cells and it plays a critical role in cell function related to immune responses and cellular interactions. CD147 interacts with several cellular components serving mechanical functions such as facilitating cell-to-cell communication and contributing to the stability of cell structures. The protein is ubiquitously expressed but exhibits higher levels in tissues like the brain skin and liver.

Biological function summary

CD147 engages in multiple roles beyond its mechanical functions. It functions as a part of protein complexes and is involved in processes such as regulation of matrix metalloproteinases (MMPs) which are pivotal in tissue remodeling and repair. It can modulate inflammatory responses and is essential for cellular signaling pathways that affect cellular metabolism and growth. The involvement of CD147 in immune responses indicates its importance in various physiological processes.

Pathways

CD147 plays a significant role in the MAPK and NF-kB signaling pathways which are fundamental for controlling inflammatory responses and cell survival. It interacts with proteins such as integrins and cyclophilins which are important for mediating these pathways. These interactions highlight CD147's impact on cellular dynamics and its potential role in modulating the cellular environment in response to external stimuli.

Associated diseases and disorders

CD147 has been implicated in cancer promotion and progression as well as inflammatory diseases like rheumatoid arthritis. Its overexpression is often observed in tumors where it interacts with MMPs to facilitate tumor invasion and metastasis. In inflammatory conditions CD147 can influence the behavior of proteins like cytokines exacerbating symptoms and contributing to disease severity. These associations make CD147 a potential target for therapeutic intervention in cancer and inflammatory diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com