JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB119114

Anti-CD147 antibody [MEM-M6/6] - Low endotoxin, Azide free

Be the first to review this product! Submit a review

|

(8 Publications)

Mouse Monoclonal CD147 antibody. Carrier free. Suitable for ICC/IF, Flow Cyt and reacts with Simian Virus 40, Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human BSG.

View Alternative Names

CD147, UNQ6505/PRO21383, BSG, Basigin, 5F7, Collagenase stimulatory factor, Extracellular matrix metalloproteinase inducer, Hepatoma-associated antigen, Leukocyte activation antigen M6, OK blood group antigen, Tumor cell-derived collagenase stimulatory factor, EMMPRIN, HAb18G, TCSF

2 Images
Flow Cytometry - Anti-CD147 antibody [MEM-M6/6] - Low endotoxin, Azide free (AB119114)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-CD147 antibody [MEM-M6/6] - Low endotoxin, Azide free (AB119114)

Human peripheral blood lymphocytes stained with ab119114 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID : 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab119114, 1μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood lymphocytes.

Immunocytochemistry - Anti-CD147 antibody [MEM-M6/6] - Low endotoxin, Azide free (AB119114)
  • ICC

Unknown

Immunocytochemistry - Anti-CD147 antibody [MEM-M6/6] - Low endotoxin, Azide free (AB119114)

ICC/IF image of ab119114 stained SV40 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab119114, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM-M6/6

Isotype

IgG1

Carrier free

Yes

Reacts with

Human, Simian Virus 40

Applications

Flow Cyt, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human BSG. The exact immunogen used to generate this antibody is proprietary information.

P35613

Specificity

ab119114 recognizes Ig domain D2 (membrane proximal) of CD147 (Neurothelin).

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" }, "Simian Virus 40": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }
style
left-column

Product details

Endotoxin level is less than 10 EU/mg of the protein, as determined by the LAL test.
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
ab119114 is Purified from hybridoma culture supernatant by protein-A affinity chromatography. Purity is > 95% (by SDS-PAGE), 0.2 µm filter sterilized.
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD147 also referred to as EMMPRIN or basigin is a transmembrane glycoprotein with a molecular weight of roughly 50–60 kDa. This protein is found on the surface of many cell types including leukocytes platelets and endothelial cells and it plays a critical role in cell function related to immune responses and cellular interactions. CD147 interacts with several cellular components serving mechanical functions such as facilitating cell-to-cell communication and contributing to the stability of cell structures. The protein is ubiquitously expressed but exhibits higher levels in tissues like the brain skin and liver.
Biological function summary

CD147 engages in multiple roles beyond its mechanical functions. It functions as a part of protein complexes and is involved in processes such as regulation of matrix metalloproteinases (MMPs) which are pivotal in tissue remodeling and repair. It can modulate inflammatory responses and is essential for cellular signaling pathways that affect cellular metabolism and growth. The involvement of CD147 in immune responses indicates its importance in various physiological processes.

Pathways

CD147 plays a significant role in the MAPK and NF-kB signaling pathways which are fundamental for controlling inflammatory responses and cell survival. It interacts with proteins such as integrins and cyclophilins which are important for mediating these pathways. These interactions highlight CD147's impact on cellular dynamics and its potential role in modulating the cellular environment in response to external stimuli.

CD147 has been implicated in cancer promotion and progression as well as inflammatory diseases like rheumatoid arthritis. Its overexpression is often observed in tumors where it interacts with MMPs to facilitate tumor invasion and metastasis. In inflammatory conditions CD147 can influence the behavior of proteins like cytokines exacerbating symptoms and contributing to disease severity. These associations make CD147 a potential target for therapeutic intervention in cancer and inflammatory diseases.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Isoform 1. Essential for normal retinal maturation and development (By similarity). Acts as a retinal cell surface receptor for NXNL1 and plays an important role in NXNL1-mediated survival of retinal cone photoreceptors (PubMed : 25957687). In association with glucose transporter SLC16A1/GLUT1 and NXNL1, promotes retinal cone survival by enhancing aerobic glycolysis and accelerating the entry of glucose into photoreceptors (PubMed : 25957687). May act as a potent stimulator of IL6 secretion in multiple cell lines that include monocytes (PubMed : 21620857).. Isoform 1. (Microbial infection) Erythrocyte receptor for P.falciparum RH5 which is essential for erythrocyte invasion by the merozoite stage of P.falciparum isolates 3D7 and Dd2.. Isoform 2. Signaling receptor for cyclophilins, essential for PPIA/CYPA and PPIB/CYPB-dependent signaling related to chemotaxis and adhesion of immune cells (PubMed : 11688976, PubMed : 11943775). Plays an important role in targeting monocarboxylate transporters SLC16A1/GLUT1, SLC16A11 and SLC16A12 to the plasma membrane (PubMed : 17127621, PubMed : 21778275, PubMed : 28666119). Acts as a coreceptor for vascular endothelial growth factor receptor 2 (KDR/VEGFR2) in endothelial cells enhancing its VEGFA-mediated activation and downstream signaling (PubMed : 25825981). Promotes angiogenesis through EPAS1/HIF2A-mediated up-regulation of VEGFA (isoform VEGF-165 and VEGF-121) and KDR/VEGFR2 in endothelial cells (PubMed : 19837976). Plays a key role in regulating tumor growth, invasion, metastasis and neoangiogenesis by stimulating the production and release of extracellular matrix metalloproteinases and KDR/VEGFR2 by both tumor cells and stromal cells (fibroblasts and endothelial cells) (PubMed : 11992541, PubMed : 12553375, PubMed : 15833850).. Isoform 2. (Microbial infection) Erythrocyte receptor for P.falciparum RH5 which is essential for erythrocyte invasion by the merozoite stage of P.falciparum isolates 3D7, Dd2, 7G8 and HB3 (PubMed : 22080952, PubMed : 26195724). Binding of P.falciparum RH5 results in BSG dimerization which triggers an increase in intracellular Ca(2+) in the erythrocyte (PubMed : 28409866). This essential step leads to a rearrangement of the erythrocyte cytoskeleton required for the merozoite invasion (PubMed : 28409866).. Isoform 2. (Microbial infection) Can facilitate human SARS coronavirus (SARS-CoV-1) infection via its interaction with virus-associated PPIA/CYPA.. Isoform 2. (Microbial infection) Can facilitate HIV-1 infection via its interaction with virus-associated PPIA/CYPA.. Isoform 2. (Microbial infection) First described as a receptor for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), it is not required for SARS-CoV-2 infection.. Isoform 2. (Microbial infection) Acts as a receptor for measles virus.. Isoform 2. (Microbial infection) Promotes entry of pentamer-expressing human cytomegalovirus (HCMV) into epithelial and endothelial cells.
See full target information BSG
style
left-column

Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy. Nucleic acids 26:1374-1386 PubMed34900396

2021

CD147 deficiency is associated with impairedsperm motility/acrosome reaction and offersa therapeutic target for asthenozoospermia.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Chen,Xiao Shi,Xiaofeng Li,Ruiying Diao,Qian Ma,Jing Jin,Zhuolin Qiu,Cailing Li,Mei Kuen Yu,Chaoqun Wang,Xianxin Li,Fanghong Li,David Yiu Leung Chan,Allan Zijian Zhao,Zhiming Cai,Fei Sun,Kin Lam Fok

Scientific reports 11:2225 PubMed33500482

2021

Assessing the functional impact of PfRh5 genetic diversity on ex vivo erythrocyte invasion inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Adam J Moore,Khadidiatou Mangou,Fatoumata Diallo,Seynabou D Sene,Mariama N Pouye,Bacary D Sadio,Ousmane Faye,Alassane Mbengue,Amy K Bei

Scientific reports 11:413 PubMed33432067

2021

No evidence for basigin/CD147 as a direct SARS-CoV-2 spike binding receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Jarrod Shilts,Thomas W M Crozier,Edward J D Greenwood,Paul J Lehner,Gavin J Wright

mBio 11: PubMed32317318

2020

Ehrlichia chaffeensis Uses an Invasin To Suppress Reactive Oxygen Species Generation by Macrophages via CD147-Dependent Inhibition of Vav1 To Block Rac1 Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Omid Teymournejad,Yasuko Rikihisa

International journal of molecular sciences 21: PubMed31877775

2019

SP-8356, a Novel Inhibitor of CD147-Cyclophilin A Interactions, Reduces Plaque Progression and Stabilizes Vulnerable Plaques in apoE-Deficient Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Kisoo Pahk,Chanmin Joung,Hwa Young Song,Sungeun Kim,Won-Ki Kim

Cell reports 25:2223-2233.e6 PubMed30463017

2018

Transmembrane Protease TMPRSS11B Promotes Lung Cancer Growth by Enhancing Lactate Export and Glycolytic Metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Barrett L Updegraff,Xiaorong Zhou,Yabin Guo,Mahesh S Padanad,Pei-Hsuan Chen,Chendong Yang,Jessica Sudderth,Carla Rodriguez-Tirado,Luc Girard,John D Minna,Prashant Mishra,Ralph J DeBerardinis,Kathryn A O'Donnell

Genome research 28:1372-1382 PubMed29914970

2018

Genome-scale identification of cellular pathways required for cell surface recognition.

Applications

Unspecified application

Species

Unspecified reactive species

Sumana Sharma,S Josefin Bartholdson,Amalie C M Couch,Kosuke Yusa,Gavin J Wright

Scientific reports 8:5782 PubMed29636510

2018

Plasmodium falciparum strains spontaneously switch invasion phenotype in suspension culture.

Applications

Unspecified application

Species

Unspecified reactive species

Gordon A Awandare,Prince B Nyarko,Yaw Aniweh,Reuben Ayivor-Djanie,José A Stoute
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB205450

Alexa Fluor® 488 Anti-CD147 antibody [EPR4053]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CD147 antibody [EPR4053] (AB205450)

  • 0
  • 0
Primary Antibodies

AB212057

Anti-CD147 antibody [EPR18008-67]

RabMAb|Recombinant

Flow Cytometry - Anti-CD147 antibody [EPR18008-67] (AB212057)

  • 0
  • 0
Primary Antibodies

AB108308

Anti-CD147 antibody [EPR4053]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD147 antibody [EPR4053] (AB108308)

  • 3
  • 1
Primary Antibodies

AB222389

Anti-CD147 antibody [EPR18008-8] - BSA and Azide free

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-CD147 antibody [EPR18008-8] - BSA and Azide free (AB222389)

  • 0
  • 0
Primary Antibodies

AB666

Anti-CD147 antibody [MEM-M6/1]

BOND RX™ Validated|KO Validated

Flow Cytometry - Anti-CD147 antibody [MEM-M6/1] (AB666)

  • 4
  • 5
Primary Antibodies

AB188190

Anti-CD147 antibody [EPR18008-8]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-CD147 antibody [EPR18008-8] (AB188190)

  • 0
  • 0

Complementary Products

Assay Kits

AB219631

Human EMMPRIN ELISA Kit

KO Validated|Recombinant|SimpleStep

Sandwich ELISA - Human EMMPRIN ELISA Kit (AB219631)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB134045

Anti-CD63 antibody [EPR5702] - Late Endosome Marker

RabMAb|Recombinant|KO Validated|Lab Essentials|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [EPR5702] - Late Endosome Marker (AB134045)

  • 3
  • 6
Primary Antibodies

AB92536

Anti-MMP2 antibody [EPR1184]

RabMAb|Recombinant

Western blot - Anti-MMP2 antibody [EPR1184] (AB92536)

  • 4
  • 6

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com