Skip to main content

Mouse Monoclonal CD147 antibody. Suitable for WB, ICC/IF and reacts with Human, African green monkey samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human BSG.

Be the first to review this product! Submit a review

Images

Western blot - Anti-CD147 antibody [OTI9B10] (AB119020), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CD147 antibody [OTI9B10] (AB119020), expandable thumbnail
  • Western blot - Anti-CD147 antibody [OTI9B10] (AB119020), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human BSG. Database link P35613

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWBICC/IF
Human
Not recommended
Tested
Expected
African green monkey
Not recommended
Expected
Tested

Not recommended
Not recommended

Species
African green monkey
Dilution info
-
Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Species
Human
Dilution info
-
Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species
Human
Dilution info
1/2000
Notes

-

Expected
Expected

Species
African green monkey
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
African green monkey
Dilution info
1/100
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

Isoform 1. Essential for normal retinal maturation and development (By similarity). Acts as a retinal cell surface receptor for NXNL1 and plays an important role in NXNL1-mediated survival of retinal cone photoreceptors (PubMed:25957687). In association with glucose transporter SLC16A1/GLUT1 and NXNL1, promotes retinal cone survival by enhancing aerobic glycolysis and accelerating the entry of glucose into photoreceptors (PubMed:25957687). May act as a potent stimulator of IL6 secretion in multiple cell lines that include monocytes (PubMed:21620857). Isoform 1. (Microbial infection) Erythrocyte receptor for P.falciparum RH5 which is essential for erythrocyte invasion by the merozoite stage of P.falciparum isolates 3D7 and Dd2. Isoform 2. Signaling receptor for cyclophilins, essential for PPIA/CYPA and PPIB/CYPB-dependent signaling related to chemotaxis and adhesion of immune cells (PubMed:11688976, PubMed:11943775). Plays an important role in targeting monocarboxylate transporters SLC16A1/GLUT1, SLC16A11 and SLC16A12 to the plasma membrane (PubMed:17127621, PubMed:21778275, PubMed:28666119). Acts as a coreceptor for vascular endothelial growth factor receptor 2 (KDR/VEGFR2) in endothelial cells enhancing its VEGFA-mediated activation and downstream signaling (PubMed:25825981). Promotes angiogenesis through EPAS1/HIF2A-mediated up-regulation of VEGFA (isoform VEGF-165 and VEGF-121) and KDR/VEGFR2 in endothelial cells (PubMed:19837976). Plays a key role in regulating tumor growth, invasion, metastasis and neoangiogenesis by stimulating the production and release of extracellular matrix metalloproteinases and KDR/VEGFR2 by both tumor cells and stromal cells (fibroblasts and endothelial cells) (PubMed:11992541, PubMed:12553375, PubMed:15833850). Isoform 2. (Microbial infection) Erythrocyte receptor for P.falciparum RH5 which is essential for erythrocyte invasion by the merozoite stage of P.falciparum isolates 3D7, Dd2, 7G8 and HB3 (PubMed:22080952, PubMed:26195724). Binding of P.falciparum RH5 results in BSG dimerization which triggers an increase in intracellular Ca(2+) in the erythrocyte (PubMed:28409866). This essential step leads to a rearrangement of the erythrocyte cytoskeleton required for the merozoite invasion (PubMed:28409866). Isoform 2. (Microbial infection) Can facilitate human SARS coronavirus (SARS-CoV-1) infection via its interaction with virus-associated PPIA/CYPA. Isoform 2. (Microbial infection) Can facilitate HIV-1 infection via its interaction with virus-associated PPIA/CYPA. Isoform 2. (Microbial infection) First described as a receptor for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), it is not required for SARS-CoV-2 infection. Isoform 2. (Microbial infection) Acts as a receptor for measles virus. Isoform 2. (Microbial infection) Promotes entry of pentamer-expressing human cytomegalovirus (HCMV) into epithelial and endothelial cells.

Alternative names

Recommended products

Mouse Monoclonal CD147 antibody. Suitable for WB, ICC/IF and reacts with Human, African green monkey samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human BSG.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Full Length Protein corresponding to Human BSG. Database link P35613
Clone number
OTI9B10
Purification technique
Affinity purification
Concentration
Loading...
Purification notes

Purified from cell culture supernatant by affinity chromatography

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

The clone number has been updated from 9B10 to OTI9B10, both clone numbers name the same clone.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CD147 also referred to as EMMPRIN or basigin is a transmembrane glycoprotein with a molecular weight of roughly 50–60 kDa. This protein is found on the surface of many cell types including leukocytes platelets and endothelial cells and it plays a critical role in cell function related to immune responses and cellular interactions. CD147 interacts with several cellular components serving mechanical functions such as facilitating cell-to-cell communication and contributing to the stability of cell structures. The protein is ubiquitously expressed but exhibits higher levels in tissues like the brain skin and liver.

Biological function summary

CD147 engages in multiple roles beyond its mechanical functions. It functions as a part of protein complexes and is involved in processes such as regulation of matrix metalloproteinases (MMPs) which are pivotal in tissue remodeling and repair. It can modulate inflammatory responses and is essential for cellular signaling pathways that affect cellular metabolism and growth. The involvement of CD147 in immune responses indicates its importance in various physiological processes.

Pathways

CD147 plays a significant role in the MAPK and NF-kB signaling pathways which are fundamental for controlling inflammatory responses and cell survival. It interacts with proteins such as integrins and cyclophilins which are important for mediating these pathways. These interactions highlight CD147's impact on cellular dynamics and its potential role in modulating the cellular environment in response to external stimuli.

Associated diseases and disorders

CD147 has been implicated in cancer promotion and progression as well as inflammatory diseases like rheumatoid arthritis. Its overexpression is often observed in tumors where it interacts with MMPs to facilitate tumor invasion and metastasis. In inflammatory conditions CD147 can influence the behavior of proteins like cytokines exacerbating symptoms and contributing to disease severity. These associations make CD147 a potential target for therapeutic intervention in cancer and inflammatory diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-CD147 antibody [OTI9B10] (ab119020), expandable thumbnail

    Western blot - Anti-CD147 antibody [OTI9B10] (ab119020)

    Lanes 1 - 4: Merged signal (red and green). Green - ab119020 observed at 55-70 kDa. Red - loading control Anti-alpha Tubulin antibody [EP1332Y] - Loading Control ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55kDa.

    ab119020 was shown to react with CD147 in wild-type A549 cells in western blot with loss of signal observed in BSG knockout cell line Human BSG (CD147) knockout A549 cell line ab273748 (knockout cell lysate Human BSG (CD147) knockout A549 cell lysate ab275500). Wild-type and BSG knockout A549 cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab119020 and Anti-alpha Tubulin antibody [EP1332Y] - Loading Control ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4°C at a 1 in 2000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-CD147 antibody [OTI9B10] (ab119020) at 1/2000 dilution

    Lane 1: Wild-type A549 cell lysate at 30 µg

    Lane 2: BSG knockout A549 cell lysate at 30 µg

    Lane 2: Western blot - Human BSG (CD147) knockout A549 cell line (Human BSG (CD147) knockout A549 cell line ab273748)

    Lane 3: Raji cell lysate at 30 µg

    Lane 4: Jurkat cell lysate at 30 µg

    Performed under reducing conditions.

    Predicted band size: 42 kDa

    Observed band size: 55-70 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-CD147 antibody [OTI9B10] (ab119020), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CD147 antibody [OTI9B10] (ab119020)

    ab119020, at a 1/100 dilution, staining CD147 in COS7 cells transiently transfected with pCMV6-ENTRY CD147 by Immunofluorescence.

  • Western blot - Anti-CD147 antibody [OTI9B10] (ab119020), expandable thumbnail

    Western blot - Anti-CD147 antibody [OTI9B10] (ab119020)

    All lanes: Western blot - Anti-CD147 antibody [OTI9B10] (ab119020) at 1/2000 dilution

    Lane 1: HEK293T cell lysate transfected with pCMV6-ENTRY control at 5 µg

    Lane 2: HEK293T cell lysate transfected with pCMV6-ENTRY CD147 cDNA at 5 µg

    Predicted band size: 32 kDa, 42 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com