AB20137

Anti-CD15 antibody [28]

Name: Anti-CD15 antibody [28] (ab20137) | Abcam
Brand: Abcam Limited
SKU: AB20137
Price: 535 USD
Availability: InStock

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(5 Publications)

Mouse Monoclonal CD15 antibody. Suitable for IP, Flow Cyt, IHC-Fr and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Cell preparation containing CD15 protein.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

Isotype

IgM

Light chain type

unknown

Carrier free

Applications

IP, IHC-Fr, Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Specific to CD15. 28 is useful for leukaemia typing and purification of stem cells.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igm-b11-7-isotype-control-ab91545'>ab91545</a> - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "Note: samples should be acetone fixed." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD15 also known as Lewis x or 3-fucosyl-N-acetyllactosamine is a carbohydrate antigen with a molecular mass typically not individually defined due to its nature as a glycan. CD15 is expressed on the surface of many human cells including neutrophils and various epithelial cells. It is commonly associated with granulocytes and is used as a cell surface marker. The presence of CD15 can be identified using CD15 antibodies through techniques such as CD15 flow cytometry and CD15 APC labeling.

Biological function summary

CD15 is important in cell-cell interaction mechanisms. It plays a role in mediating adhesion processes particularly in immune responses where it aids neutrophils in adhering to vascular endothelium and transmigrating to sites of infection or inflammation. As a glycoconjugate CD15 forms part of a larger glycoprotein complex on the cell surface interacting with other proteins to facilitate cellular responses and movement.

Pathways

CD15's function as an adhesion molecule ties into key cellular processes such as the selectin pathway. In this pathway it interacts with proteins like E-selectin and L-selectin contributing to leukocyte rolling and extravasation during the immune response. Additionally CD15 is implicated in the signaling pathways that mediate inflammatory responses where its role in neutrophil dynamics is important.

CD15 has notable implications in Hodgkin's lymphoma and certain myeloid leukemias. It is frequently used as a diagnostic marker in these conditions where its overexpression or abnormal expression patterns may signify disease presence or progression. CD15's interaction with other proteins such as CD30 in Hodgkin's lymphoma highlights its utility in understanding the pathological mechanisms and potential therapeutic targets of these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information CD15

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 11:915 PubMed33441653

2021

Quantitative STAU2 measurement in lymphocytes for breast cancer risk assessment.

Applications

Unspecified application

Species

Unspecified reactive species

Charoenchai Puttipanyalears,Sikrit Denariyakoon,Phonthep Angsuwatcharakon,Vitavat Aksornkitti,Mawin Vongsaisuwan,Sutasinee Asayut,Somchai Thanasitthichai,Narisorn Kongruttanachok,Chatchawit Aporntewan,Apiwat Mutirangura

International journal of molecular medicine 43:1687-1698 PubMed30816446

2019

Co‑expression of Lewis y antigen and CD147 in epithelial ovarian cancer is correlated with malignant progression and poor prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Juanjuan Liu,Qi Liu,Yanyan Wang,Miao Liu,Yue Qi,Jian Gao,Bei Lin

Translational oncology 5:141-54 PubMed22741033

2012

A Prominin-1-Rich Pediatric Glioblastoma: Biologic Behavior Is Determined by Oxygen Tension-Modulated CD133 Expression but Not Accompanied by Underlying Molecular Profiles.

Applications

ICC/IF, Flow Cyt

Species

Human, Human

Laura K Donovan,Nicola E Potter,Tracy Warr,Geoffrey J Pilkington

Immunology 53:753-67 PubMed6389324

1984

Monoclonal antibodies specific for human monocytes, granulocytes and endothelium.

Applications

Unspecified application

Species

Unspecified reactive species

N Hogg,S MacDonald,M Slusarenko,P C Beverley

Blood 63:566-73 PubMed6365201

1984

Monoclonal antibodies differentiating between monocytic and nonmonocytic variants of AML.

Applications

Unspecified application

Species

Unspecified reactive species

D C Linch,C Allen,P C Beverley,A G Bynoe,C S Scott,N Hogg

View all publications

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