Mouse Monoclonal CD15 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Cell preparation containing CD15 protein.
pH: 7.2 - 7.4
Constituents: PBS
| IHC-P | |
|---|---|
| Human | Tested |
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.50000-1.00000 µg/mL | Notes Primary incubation for 30 minutes at RT. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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Mouse Monoclonal CD15 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Cell preparation containing CD15 protein.
pH: 7.2 - 7.4
Constituents: PBS
Purified from Bioreactor Concentrate.
ab269779 is a carrier free version of Anti-CD15 antibody [BRA-4F1] ab233868.
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Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
CD15 also known as Lewis x or 3-fucosyl-N-acetyllactosamine is a carbohydrate antigen with a molecular mass typically not individually defined due to its nature as a glycan. CD15 is expressed on the surface of many human cells including neutrophils and various epithelial cells. It is commonly associated with granulocytes and is used as a cell surface marker. The presence of CD15 can be identified using CD15 antibodies through techniques such as CD15 flow cytometry and CD15 APC labeling.
CD15 is important in cell-cell interaction mechanisms. It plays a role in mediating adhesion processes particularly in immune responses where it aids neutrophils in adhering to vascular endothelium and transmigrating to sites of infection or inflammation. As a glycoconjugate CD15 forms part of a larger glycoprotein complex on the cell surface interacting with other proteins to facilitate cellular responses and movement.
CD15's function as an adhesion molecule ties into key cellular processes such as the selectin pathway. In this pathway it interacts with proteins like E-selectin and L-selectin contributing to leukocyte rolling and extravasation during the immune response. Additionally CD15 is implicated in the signaling pathways that mediate inflammatory responses where its role in neutrophil dynamics is important.
CD15 has notable implications in Hodgkin's lymphoma and certain myeloid leukemias. It is frequently used as a diagnostic marker in these conditions where its overexpression or abnormal expression patterns may signify disease presence or progression. CD15's interaction with other proteins such as CD30 in Hodgkin's lymphoma highlights its utility in understanding the pathological mechanisms and potential therapeutic targets of these disorders.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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