AB220182

Anti-CD15 antibody [FUT4/815]

Name: Anti-CD15 antibody [FUT4/815] (ab220182) | Abcam
Brand: Abcam Limited
SKU: AB220182
Price: 525 USD
Availability: InStock

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(2 Publications)

Mouse Monoclonal CD15 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Cell preparation containing CD15 protein.

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD15 antibody [FUT4/815] (AB220182)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD15 antibody [FUT4/815] (AB220182)

Immunohistochemical analysis of formalin fixed paraffin embedded Human Hodgkin's Lymphoma tissue labeling CD15 with ab220182 at 1 μg/ml.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

FUT4/815

Isotype

IgM

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p>Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 45 min at 95°C followed by cooling at room temperature for 20 minutes.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

purified from Bioreactor Concentrate

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD15 also known as Lewis x or 3-fucosyl-N-acetyllactosamine is a carbohydrate antigen with a molecular mass typically not individually defined due to its nature as a glycan. CD15 is expressed on the surface of many human cells including neutrophils and various epithelial cells. It is commonly associated with granulocytes and is used as a cell surface marker. The presence of CD15 can be identified using CD15 antibodies through techniques such as CD15 flow cytometry and CD15 APC labeling.

Biological function summary

CD15 is important in cell-cell interaction mechanisms. It plays a role in mediating adhesion processes particularly in immune responses where it aids neutrophils in adhering to vascular endothelium and transmigrating to sites of infection or inflammation. As a glycoconjugate CD15 forms part of a larger glycoprotein complex on the cell surface interacting with other proteins to facilitate cellular responses and movement.

Pathways

CD15's function as an adhesion molecule ties into key cellular processes such as the selectin pathway. In this pathway it interacts with proteins like E-selectin and L-selectin contributing to leukocyte rolling and extravasation during the immune response. Additionally CD15 is implicated in the signaling pathways that mediate inflammatory responses where its role in neutrophil dynamics is important.

CD15 has notable implications in Hodgkin's lymphoma and certain myeloid leukemias. It is frequently used as a diagnostic marker in these conditions where its overexpression or abnormal expression patterns may signify disease presence or progression. CD15's interaction with other proteins such as CD30 in Hodgkin's lymphoma highlights its utility in understanding the pathological mechanisms and potential therapeutic targets of these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information CD15

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:7281 PubMed36435834

2022

ARID1A loss induces polymorphonuclear myeloid-derived suppressor cell chemotaxis and promotes prostate cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Ni Li,Qiuli Liu,Ying Han,Siyu Pei,Bisheng Cheng,Junyu Xu,Xiang Miao,Qiang Pan,Hanling Wang,Jiacheng Guo,Xuege Wang,Guoying Zhang,Yannan Lian,Wei Zhang,Yi Zang,Minjia Tan,Qintong Li,Xiaoming Wang,Yichuan Xiao,Guohong Hu,Jun Jiang,Hai Huang,Jun Qin

Journal of vascular research 58:267-276 PubMed33946068

2021

Protective Effects of 1,25 Dihydroxyvitamin D3 against High-Glucose-Induced Damage in Human Umbilical Vein Endothelial Cells Involve Activation of Nrf2 Antioxidant Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Meifang Wu,Ying Wu,Kaizu Xu,Liming Lin

View all publications

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