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AB665

Anti-CD15 antibody [MEM-158]

5

(1 Review)

|

(3 Publications)

Mouse Monoclonal CD15 antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Cell preparation containing CD15 protein.
2 Images
Flow Cytometry - Anti-CD15 antibody [MEM-158] (AB665)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD15 antibody [MEM-158] (AB665)

Flow cytometry surface staining pattern of human peripheral blood stained using Anti-CD15 antibody [MEM-158] (ab665) at 0.3 µg/mL.

Flow Cytometry - Anti-CD15 antibody [MEM-158] (AB665)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD15 antibody [MEM-158] (AB665)

Flow Cytometry analysis of human peripheral whole blood labeling CD15 (surface staining) with ab665 at 0.3 μg/mL. CD15 positive human neutrophil granulocytes (red-filled). CD15 negative lymphocytes (black-dashed).

  • 519 FITC

    FITC Anti-CD15 antibody [MEM-158]

  • Biotin

    Biotin Anti-CD15 antibody [MEM-158]

  • 695 PerCP/Cy5.5®

    PerCP/Cy5.5® Anti-CD15 antibody [MEM-158]

  • 660 APC

    APC Anti-CD15 antibody [MEM-158]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM-158

Isotype

IgM

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-4 µg/mL", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igm-b11-7-isotype-control-ab91545'>ab91545</a> - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Proprietary technique
Purification notes
Purified from TCS. Purified by precipitation and chromatography. Purity >95% by SDS-PAGE.
Storage buffer
pH: 8 Preservative: 0.097% Sodium azide Constituents: Tris buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD15 also known as Lewis x or 3-fucosyl-N-acetyllactosamine is a carbohydrate antigen with a molecular mass typically not individually defined due to its nature as a glycan. CD15 is expressed on the surface of many human cells including neutrophils and various epithelial cells. It is commonly associated with granulocytes and is used as a cell surface marker. The presence of CD15 can be identified using CD15 antibodies through techniques such as CD15 flow cytometry and CD15 APC labeling.
Biological function summary

CD15 is important in cell-cell interaction mechanisms. It plays a role in mediating adhesion processes particularly in immune responses where it aids neutrophils in adhering to vascular endothelium and transmigrating to sites of infection or inflammation. As a glycoconjugate CD15 forms part of a larger glycoprotein complex on the cell surface interacting with other proteins to facilitate cellular responses and movement.

Pathways

CD15's function as an adhesion molecule ties into key cellular processes such as the selectin pathway. In this pathway it interacts with proteins like E-selectin and L-selectin contributing to leukocyte rolling and extravasation during the immune response. Additionally CD15 is implicated in the signaling pathways that mediate inflammatory responses where its role in neutrophil dynamics is important.

CD15 has notable implications in Hodgkin's lymphoma and certain myeloid leukemias. It is frequently used as a diagnostic marker in these conditions where its overexpression or abnormal expression patterns may signify disease presence or progression. CD15's interaction with other proteins such as CD30 in Hodgkin's lymphoma highlights its utility in understanding the pathological mechanisms and potential therapeutic targets of these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information CD15
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

CNS neuroscience & therapeutics 29:2206-2222 PubMed37032635

2023

Inhibition of TANK-binding kinase1 attenuates the astrocyte-mediated neuroinflammatory response through YAP signaling after spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Wenbin Zhang,Mengxian Jia,Jiashu Lian,Sheng Lu,Jian Zhou,Ziwei Fan,Zhoule Zhu,Yaozhi He,Changgang Huang,Mingyu Zhu,Jian Wang,Ying Wang,Zhihui Huang,Honglin Teng

Biology of reproduction 108:292-303 PubMed36401880

2022

Lewis x-carrying O-glycans are candidate modulators for conceptus attachment in pigs†.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Han,Yulu Yue,Weiwei Wang,Feiyu Wang,Wengang Chai,Shuhong Zhao,Mei Yu

eLife 10: PubMed34219648

2021

Dynamic persistence of UPEC intracellular bacterial communities in a human bladder-chip model of urinary tract infection.

Applications

Unspecified application

Species

Unspecified reactive species

Kunal Sharma,Neeraj Dhar,Vivek V Thacker,Thomas M Simonet,Francois Signorino-Gelo,Graham W Knott,John D McKinney
View all publications
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