Anti-CD15 antibody [SP159]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD15 antibody [SP159] (AB135377)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human spleen tissue sections labeling CD15 with ab135377 at 1/50 dilution (3.14 μg/ml). Heat mediated antigen retrieval with Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human spleen, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab135377 for 30 mins at room temperature.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD15 antibody [SP159] (AB135377)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human Hodgkin's lymphoma tissue sections labeling CD15 with ab135377 at 1/50 dilution (3.14 μg/ml). Heat mediated antigen retrieval with Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human Hodgkin's lymphoma, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab135377 for 30 mins at room temperature.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD15 antibody [SP159] (AB135377)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human Hodgkin's Lymphoma tissue labelling CD15 with ab135377 at 1/100 dilution.

• Flow Cyt

Unknown

Flow Cytometry - Anti-CD15 antibody [SP159] (AB135377)

Flow cytometry analysis of THP-1 (human acute monocytic leukemia) labeling CD15 with purified ab135377 at 1/20 dilution (7.85 μg/ml) (red). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150081) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlableled control - Unlabelled cells (blue).

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD15 antibody [SP159] (AB135377)

Flow cytometric analysis of rabbit anti-CD15 (SP159) antibody ab135377 (1/200) in HL-60 cells(green) compared to negative control of rabbit IgG (blue).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD15 antibody [SP159] (AB135377)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cervix carcinoma tissue sections labeling CD15 with ab135377 at 1/50 dilution (3.14 μg/ml). Heat mediated antigen retrieval with Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human cervix carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab135377 for 30 mins at room temperature.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-CD15 antibody [SP159] (AB135377)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human spleen tissue staining CD15 with ab135377 at a 1/50 dilution and ab272713 anti-S100A12/CGRP used at 1/5000 dilution. Opal Polymer HRP Ms + Rb was used as a secondary, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Panel A : merged staining of anti-CD15 (green; Opal^™520), anti-S100A12/CGRP (magenta; Opal^™690) on human spleen.
Panel B : anti-CD15 stained granulocytes and monocytes on human spleen.
Panel C : anti-S100A12/CGRP stained neutrophils and monocytes on human spleen.
Panel D : Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab135377 and ab272713 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-CD15 antibody [SP159] (AB135377)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human Hodgkin's lymphoma tissue staining CD15 with ab135377 at a 1/50 dilution and ab272713 anti-S100A12/CGRP used at 1/5000 dilution. Opal Polymer HRP Ms + Rb was used as a secondary, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Panel A : merged staining of anti-CD15 (green; Opal^™520) and anti-S100A12/CGRP (magenta; Opal^™690) on human Hodgkin's lymphoma.
Panel B : anti-CD15 stained granulocytes and monocytes on human Hodgkin's lymphoma.
Panel C : anti-S100A12/CGRP stained neutrophils and monocytes on human Hodgkin's lymphoma.
Panel D : Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab135377 and ab272713 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• Flow Cyt

Lab

Flow Cytometry - Anti-CD15 antibody [SP159] (AB135377)

Flow cytometric analysis of human neutrophils cells labelling CD15 with ab135377 at 1/2000 compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor^® 488 (ab150081) at 1/5000 was used as the secondary antibody.

Gated on viable cells.