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Rabbit Recombinant Monoclonal CD15 antibody. Carrier free. Suitable for Flow Cyt, IHC-P and reacts with Human, Mouse samples.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD15 antibody [SP159] - BSA and Azide free (AB240092), expandable thumbnail
  • Flow Cytometry - Anti-CD15 antibody [SP159] - BSA and Azide free (AB240092), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD15 antibody [SP159] - BSA and Azide free (AB240092), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD15 antibody [SP159] - BSA and Azide free (AB240092), expandable thumbnail
  • Flow Cytometry - Anti-CD15 antibody [SP159] - BSA and Azide free (AB240092), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytIHC-P
Human
Tested
Tested
Mouse
Expected
Tested

Tested
Tested

Species

Human

Dilution info

-

Notes

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

Perform heat mediated antigen retrieval by boiling tissue section in 1mM EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min. Incubate with primary antibody for 10 minutes at room temperature.

Species

Human

Dilution info

-

Notes

Perform heat mediated antigen retrieval by boiling tissue section in 1mM EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min. Incubate with primary antibody for 10 minutes at room temperature.

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Rabbit Recombinant Monoclonal CD15 antibody. Carrier free. Suitable for Flow Cyt, IHC-P and reacts with Human, Mouse samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

SP159

Purification technique

Affinity purification Protein A/G

Concentration
Loading...
Purification notes

Purified from TCS by protein A/G.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab240092 is the carrier-free version of Anti-CD15 antibody [SP159] ab135377.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD15 also known as Lewis x or 3-fucosyl-N-acetyllactosamine is a carbohydrate antigen with a molecular mass typically not individually defined due to its nature as a glycan. CD15 is expressed on the surface of many human cells including neutrophils and various epithelial cells. It is commonly associated with granulocytes and is used as a cell surface marker. The presence of CD15 can be identified using CD15 antibodies through techniques such as CD15 flow cytometry and CD15 APC labeling.

Biological function summary

CD15 is important in cell-cell interaction mechanisms. It plays a role in mediating adhesion processes particularly in immune responses where it aids neutrophils in adhering to vascular endothelium and transmigrating to sites of infection or inflammation. As a glycoconjugate CD15 forms part of a larger glycoprotein complex on the cell surface interacting with other proteins to facilitate cellular responses and movement.

Pathways

CD15's function as an adhesion molecule ties into key cellular processes such as the selectin pathway. In this pathway it interacts with proteins like E-selectin and L-selectin contributing to leukocyte rolling and extravasation during the immune response. Additionally CD15 is implicated in the signaling pathways that mediate inflammatory responses where its role in neutrophil dynamics is important.

Associated diseases and disorders

CD15 has notable implications in Hodgkin's lymphoma and certain myeloid leukemias. It is frequently used as a diagnostic marker in these conditions where its overexpression or abnormal expression patterns may signify disease presence or progression. CD15's interaction with other proteins such as CD30 in Hodgkin's lymphoma highlights its utility in understanding the pathological mechanisms and potential therapeutic targets of these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD15 antibody [SP159] - BSA and Azide free (ab240092), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD15 antibody [SP159] - BSA and Azide free (ab240092)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse kidney tissue sections labeling CD15 with Anti-CD15 antibody [SP159] ab135377 at 1/50 dilution (3.14 μg/ml). Heat mediated antigen retrieval with Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on proximal tubular epithelial cells in the mouse kidney, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with Anti-CD15 antibody [SP159] ab135377 for 30 mins at room temperature. This image was generated using Anti-CD15 antibody [SP159] ab135377, the same clone, but with a different buffer formulation.

  • Flow Cytometry - Anti-CD15 antibody [SP159] - BSA and Azide free (ab240092), expandable thumbnail

    Flow Cytometry - Anti-CD15 antibody [SP159] - BSA and Azide free (ab240092)

    Flow cytometry analysis of THP-1 (human acute monocytic leukemia) labeling CD15 with purified Anti-CD15 antibody [SP159] ab135377 at 1/20 dilution (7.85 μg/ml) (red). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as a secondary antibody. Isotypecontrol - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black). Unlableled control - Unlabelled cells (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD15 antibody [SP159] ab135377).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD15 antibody [SP159] - BSA and Azide free (ab240092), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD15 antibody [SP159] - BSA and Azide free (ab240092)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cervix carcinoma tissue sections labeling CD15 with Anti-CD15 antibody [SP159] ab135377 at 1/50 dilution (3.14 μg/ml). Heat mediated antigen retrieval with Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human cervix carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with Anti-CD15 antibody [SP159] ab135377 for 30 mins at room temperature. This image was generated using Anti-CD15 antibody [SP159] ab135377, the same clone, but with a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD15 antibody [SP159] - BSA and Azide free (ab240092), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD15 antibody [SP159] - BSA and Azide free (ab240092)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human Hodgkin's lymphoma tissue sections labeling CD15 with Anti-CD15 antibody [SP159] ab135377 at 1/50 dilution (3.14 μg/ml). Heat mediated antigen retrieval with Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human Hodgkin's lymphoma, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with Anti-CD15 antibody [SP159] ab135377 for 30 mins at room temperature. This image was generated using Anti-CD15 antibody [SP159] ab135377, the same clone, but with a different buffer formulation.

  • Flow Cytometry - Anti-CD15 antibody [SP159] - BSA and Azide free (ab240092), expandable thumbnail

    Flow Cytometry - Anti-CD15 antibody [SP159] - BSA and Azide free (ab240092)

    Flow cytometric analysis of rabbit anti-CD15 (SP159) antibody  Anti-CD15 antibody [SP159] ab135377 (1/200) in HL-60 cells(green) compared to negative control of rabbit IgG (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD15 antibody [SP159] ab135377).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD15 antibody [SP159] - BSA and Azide free (ab240092), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD15 antibody [SP159] - BSA and Azide free (ab240092)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human spleen tissue sections labeling CD15 with Anti-CD15 antibody [SP159] ab135377 at 1/50 dilution (3.14 μg/ml). Heat mediated antigen retrieval with Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human spleen, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with Anti-CD15 antibody [SP159] ab135377 for 30 mins at room temperature. This image was generated using Anti-CD15 antibody [SP159] ab135377, the same clone, but with a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD15 antibody [SP159] - BSA and Azide free (ab240092), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD15 antibody [SP159] - BSA and Azide free (ab240092)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma tissue labelling CD15 with Anti-CD15 antibody [SP159] ab135377 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-CD15 antibody [SP159] ab135377).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com