Name: Anti-CD15 antibody [SP159] - BSA and Azide free
SKU: ab240092
Rating: 5 (1 reviews)

Rabbit Recombinant Monoclonal CD15 antibody. Carrier free. Suitable for IHC-P, Flow Cyt, mIHC and reacts with Human samples.

Images

Flow Cytometry - Anti-CD15 antibody [SP159] - BSA and Azide free (AB240092), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	Flow Cyt	mIHC
Human	Tested	Tested	Tested
Mouse	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat mediated antigen retrieval by boiling tissue section in 1mM EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min. Incubate with primary antibody for 10 minutes at room temperature.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -

Associated Products

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15 products for Alternative Product

3 products for Alternative Version

Target data

See full target information CD15

Recommended products

Rabbit Recombinant Monoclonal CD15 antibody. Carrier free. Suitable for IHC-P, Flow Cyt, mIHC and reacts with Human samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: SP159
Purification technique: Affinity purification Protein A/G
Concentration
Purification notes: Purified from TCS by protein A/G.

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: Do Not Freeze

Notes

ab240092 is the carrier-free version of Anti-CD15 antibody [SP159] ab135377.

What are the advantages of a recombinant monoclonal antibody?

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD15 also known as Lewis x or 3-fucosyl-N-acetyllactosamine is a carbohydrate antigen with a molecular mass typically not individually defined due to its nature as a glycan. CD15 is expressed on the surface of many human cells including neutrophils and various epithelial cells. It is commonly associated with granulocytes and is used as a cell surface marker. The presence of CD15 can be identified using CD15 antibodies through techniques such as CD15 flow cytometry and CD15 APC labeling.

Biological function summary

CD15 is important in cell-cell interaction mechanisms. It plays a role in mediating adhesion processes particularly in immune responses where it aids neutrophils in adhering to vascular endothelium and transmigrating to sites of infection or inflammation. As a glycoconjugate CD15 forms part of a larger glycoprotein complex on the cell surface interacting with other proteins to facilitate cellular responses and movement.

Pathways

CD15's function as an adhesion molecule ties into key cellular processes such as the selectin pathway. In this pathway it interacts with proteins like E-selectin and L-selectin contributing to leukocyte rolling and extravasation during the immune response. Additionally CD15 is implicated in the signaling pathways that mediate inflammatory responses where its role in neutrophil dynamics is important.

Associated diseases and disorders

CD15 has notable implications in Hodgkin's lymphoma and certain myeloid leukemias. It is frequently used as a diagnostic marker in these conditions where its overexpression or abnormal expression patterns may signify disease presence or progression. CD15's interaction with other proteins such as CD30 in Hodgkin's lymphoma highlights its utility in understanding the pathological mechanisms and potential therapeutic targets of these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

Flow Cytometry - Anti-CD15 antibody [SP159] - BSA and Azide free (ab240092)
Flow cytometry analysis of THP-1 (human acute monocytic leukemia) labeling CD15 with purified Anti-CD15 antibody [SP159] ab135377 at 1/20 dilution (7.85 μg/ml) (red). Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as a secondary antibody. Isotypecontrol - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black). Unlableled control - Unlabelled cells (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD15 antibody [SP159] ab135377).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD15 antibody [SP159] - BSA and Azide free (ab240092)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cervix carcinoma tissue sections labeling CD15 with Anti-CD15 antibody [SP159] ab135377 at 1/50 dilution (3.14 μg/ml). Heat mediated antigen retrieval with Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human cervix carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with Anti-CD15 antibody [SP159] ab135377 for 30 mins at room temperature. This image was generated using Anti-CD15 antibody [SP159] ab135377, the same clone, but with a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD15 antibody [SP159] - BSA and Azide free (ab240092)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human Hodgkin's lymphoma tissue sections labeling CD15 with Anti-CD15 antibody [SP159] ab135377 at 1/50 dilution (3.14 μg/ml). Heat mediated antigen retrieval with Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human Hodgkin's lymphoma, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with Anti-CD15 antibody [SP159] ab135377 for 30 mins at room temperature. This image was generated using Anti-CD15 antibody [SP159] ab135377, the same clone, but with a different buffer formulation.
Flow Cytometry - Anti-CD15 antibody [SP159] - BSA and Azide free (ab240092)
Flow cytometric analysis of rabbit anti-CD15 (SP159) antibody Anti-CD15 antibody [SP159] ab135377 (1/200) in HL-60 cells(green) compared to negative control of rabbit IgG (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD15 antibody [SP159] ab135377).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD15 antibody [SP159] - BSA and Azide free (ab240092)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human spleen tissue sections labeling CD15 with Anti-CD15 antibody [SP159] ab135377 at 1/50 dilution (3.14 μg/ml). Heat mediated antigen retrieval with Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human spleen, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with Anti-CD15 antibody [SP159] ab135377 for 30 mins at room temperature. This image was generated using Anti-CD15 antibody [SP159] ab135377, the same clone, but with a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD15 antibody [SP159] - BSA and Azide free (ab240092)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma tissue labelling CD15 with Anti-CD15 antibody [SP159] ab135377 at 1/100 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-CD15 antibody [SP159] ab135377).
Multiplex immunohistochemistry - Anti-CD15 antibody [SP159] - BSA and Azide free (ab240092)
This data was developed using Anti-CD15 antibody [SP159] ab135377, the same antibody clone in a different buffer formulation.
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human Hodgkin's lymphoma tissue staining CD15 with Anti-CD15 antibody [SP159] ab135377 at a 1/50 dilution and Anti-S100A12/CGRP antibody [EPR23677-111] ab272713 anti-S100A12/CGRP used at 1/5000 dilution. Opal Polymer HRP Ms + Rb was used as a secondary, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Panel A: merged staining of anti-CD15 (green; Opal^™520) and anti-S100A12/CGRP (magenta; Opal^™690) on human Hodgkin's lymphoma.

Panel B: anti-CD15 stained granulocytes and monocytes on human Hodgkin's lymphoma.

Panel C: anti-S100A12/CGRP stained neutrophils and monocytes on human Hodgkin's lymphoma.

Panel D: Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of Anti-CD15 antibody [SP159] ab135377 and Anti-S100A12/CGRP antibody [EPR23677-111] ab272713 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Flow Cytometry - Anti-CD15 antibody [SP159] - BSA and Azide free (ab240092)
This data was developed using Anti-CD15 antibody [SP159] ab135377, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of human neutrophils cells labelling CD15 with Anti-CD15 antibody [SP159] ab135377 at 1/2000 compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).
Goat Anti-Rabbit IgG (Alexa Fluor^® 488 (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 was used as the secondary antibody.
Gated on viable cells.
Multiplex immunohistochemistry - Anti-CD15 antibody [SP159] - BSA and Azide free (ab240092)
This data was developed using Anti-CD15 antibody [SP159] ab135377, the same antibody clone in a different buffer formulation.
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human spleen tissue staining CD15 with Anti-CD15 antibody [SP159] ab135377 at a 1/50 dilution and Anti-S100A12/CGRP antibody [EPR23677-111] ab272713 anti-S100A12/CGRP used at 1/5000 dilution. Opal Polymer HRP Ms + Rb was used as a secondary, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Panel A: merged staining of anti-CD15 (green; Opal^™520), anti-S100A12/CGRP (magenta; Opal^™690) on human spleen.

Panel B: anti-CD15 stained granulocytes and monocytes on human spleen.

Panel C: anti-S100A12/CGRP stained neutrophils and monocytes on human spleen.

Panel D: Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of Anti-CD15 antibody [SP159] ab135377 and Anti-S100A12/CGRP antibody [EPR23677-111] ab272713 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.