AB27406

Anti-CD151 antibody [B-E31] - BSA and Azide free

Name: Anti-CD151 antibody [B-E31] - BSA and Azide free (ab27406) | Abcam
Brand: Abcam Limited
SKU: AB27406
Price: 490 USD
Availability: InStock

Be the first to review this product! Submit a review

(1 Publication)

Mouse Monoclonal CD151 antibody. Carrier free. Suitable for Flow Cyt, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing CD151 protein.

View Alternative Names

CD151, TSPAN24, CD151 antigen, GP27, Membrane glycoprotein SFA-1, Platelet-endothelial tetraspan antigen 3, Tetraspanin-24, PETA-3, Tspan-24

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-CD151 antibody [B-E31] - BSA and Azide free (AB27406)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CD151 antibody [B-E31] - BSA and Azide free (AB27406)

ICC/IF image of ab27406 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab27406, 10µg/ml) overnight at +4°C. The secondary antibody (green) was for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Flow Cytometry - Anti-CD151 antibody [B-E31] - BSA and Azide free (AB27406)

Flow Cyt

Unknown

Flow Cytometry - Anti-CD151 antibody [B-E31] - BSA and Azide free (AB27406)

A typical staining pattern with the ab27406 monoclonal antibody of Eahy 926 (hybridoma line derived from human endothelium and A549/8 cells) cell line.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

B-E31

Isotype

IgG1

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt, ICC/IF

applications

Immunogen

Cell preparation containing CD151 protein. The exact immunogen used to generate this antibody is proprietary information.

P48509

Specificity

Recognises the antigen PETA-3, a 32 kDa protein.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Ion exchange chromatography

Purification notes

This antibody is sterile filtered through 0.22 µm and treated to remove endotoxins. It is carrier and preservative free.

Storage buffer

pH: 7.3 - 7.5 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C|-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD151 also known as PETA-3 or SFA-1 is a member of the tetraspanin family which are small membrane proteins with molecular mass of about 28 kDa. This protein is commonly expressed on various cells such as epithelial cells endothelial cells and platelets. CD151 is known to interact with integrins especially integrin α3β1 and α6β1 playing significant roles in cell adhesion and migration. It is mostly found in areas where cell-to-cell contact is vital for maintaining tissue integrity and cellular communication.

Biological function summary

CD151 engages in the stabilization of integrin complexes which facilitates signal transduction pathways that influence cellular behaviors like proliferation differentiation and survival. It does not function alone; CD151 is part of a tetraspanin-enriched microdomain (TEM) forming complexes that include proteins such as CD9 CD63 and other integrins. These complexes bring together structural and signaling molecules on the cell membrane creating platforms for efficient signal relay.

Pathways

CD151 plays a role in pathways involving cell adhesion and tissue repair. Notably it is involved in the integrin signaling pathway which is essential for cell-matrix interactions in tissues that require adhesion for repair or restructuring. CD151 interacts with proteins like PI3K and Akt within these pathways which are central to the signaling cascades regulating cellular responses to the extracellular environment.

CD151 participation has been noted in cancer progression and metastasis. It links to the dysregulation observed in integrin functions such as those of integrin α3β1 which directly influences tumor cell invasion and metastasis in cancers like breast and prostate cancer. CD151 also exhibits connections with renal pathologies including Alport syndrome where the lack of functional basement membranes affects kidney function and it interacts with proteins like collagen IV in the disorder's progression.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling. Plays a role in various cellular and molecular mechanism through its association with both integrin and non-integrin proteins. These interactions facilitate critical cellular functions, including cell-to-cell communication, wound healing, platelet aggregation, trafficking, cell motility, and angiogenesis (PubMed : 17045834, PubMed : 24723389, PubMed : 31488507). Via interaction with JAM-A/F11R and integrin ITGA3 : ITGB1, promotes the recruitment of signaling molecules such as RAC1, CDC42 and RhoGTPases to facilitate the polarization of epithelial cells and the reorganization of the actin cytoskeleton, which are critical steps in cell migration process (PubMed : 22843693, PubMed : 35067832). Regulates the glycosylation pattern of ITGA3 : ITGB1 thereby modulating its activity (PubMed : 18852263). Plays an essential role in the maintenance of central laminin-binding integrin ITGA6 : ITGB4-containing adhesion complexes (PubMed : 31488507). Essential for the proper assembly of the glomerular and tubular basement membranes in kidney (PubMed : 15265795). Contributes to T-cell activation by modulating integrin signaling leading to activation of downstream targets PTK2 and MAPK1/MAPK3 (PubMed : 24723389).. (Microbial infection) Plays a role in human papillomavirus 16/HPV-16 endocytosis upon binding to cell surface receptor.. (Microbial infection) Plays a role in human cytomegalovirus entry into host cell by contributing to entry receptor binding, membrane fusion, or release of the capsid.

See full target information CD151

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Immunology 91:303-13 PubMed9227332

1997

Pattern of cytokine receptors expressed by human dendritic cells migrated from dermal explants.

Applications

Unspecified application

Species

Unspecified reactive species

A T Larregina,A E Morelli,E Kolkowski,N Sanjuan,M E Barboza,L Fainboim

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD151 antibody [B-E31] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-CD151 antibody [B-E31] - BSA and Azide free (AB27406)

Flow Cytometry - Anti-CD151 antibody [B-E31] - BSA and Azide free (AB27406)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise