Anti-CD151 antibody [EPR27423-31] (ab309512)

Rabbit Recombinant Monoclonal CD151 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-CD151 antibody [EPR27423-31] (AB309512), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IHC-P	ICC/IF	Flow Cyt	IP
Human	Tested	Tested	Tested	Not recommended	Not recommended
Mouse	Tested	Tested	Tested	Not recommended	Not recommended
Rat	Tested	Tested	Expected	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000	Notes -
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/50	Notes -
Species Mouse	Dilution info 1/50	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Rat, Mouse	Dilution info -	Notes -

Target data

See full target information CD151

Function

Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling. Plays a role in various cellular and molecular mechanism through its association with both integrin and non-integrin proteins. These interactions facilitate critical cellular functions, including cell-to-cell communication, wound healing, platelet aggregation, trafficking, cell motility, and angiogenesis (PubMed:17045834, PubMed:24723389, PubMed:31488507). Via interaction with JAM-A/F11R and integrin ITGA3:ITGB1, promotes the recruitment of signaling molecules such as RAC1, CDC42 and RhoGTPases to facilitate the polarization of epithelial cells and the reorganization of the actin cytoskeleton, which are critical steps in cell migration process (PubMed:22843693, PubMed:35067832). Regulates the glycosylation pattern of ITGA3:ITGB1 thereby modulating its activity (PubMed:18852263). Plays an essential role in the maintenance of central laminin-binding integrin ITGA6:ITGB4-containing adhesion complexes (PubMed:31488507). Essential for the proper assembly of the glomerular and tubular basement membranes in kidney (PubMed:15265795). Contributes to T-cell activation by modulating integrin signaling leading to activation of downstream targets PTK2 and MAPK1/MAPK3 (PubMed:24723389). (Microbial infection) Plays a role in human papillomavirus 16/HPV-16 endocytosis upon binding to cell surface receptor. (Microbial infection) Plays a role in human cytomegalovirus entry into host cell by contributing to entry receptor binding, membrane fusion, or release of the capsid.

Alternative names

CD151, TSPAN24, CD151 antigen, GP27, Membrane glycoprotein SFA-1, Platelet-endothelial tetraspan antigen 3, Tetraspanin-24, PETA-3, Tspan-24

Recommended products

Rabbit Recombinant Monoclonal CD151 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR27423-31
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD151 also known as PETA-3 or SFA-1 is a member of the tetraspanin family which are small membrane proteins with molecular mass of about 28 kDa. This protein is commonly expressed on various cells such as epithelial cells endothelial cells and platelets. CD151 is known to interact with integrins especially integrin α3β1 and α6β1 playing significant roles in cell adhesion and migration. It is mostly found in areas where cell-to-cell contact is vital for maintaining tissue integrity and cellular communication.

Biological function summary

CD151 engages in the stabilization of integrin complexes which facilitates signal transduction pathways that influence cellular behaviors like proliferation differentiation and survival. It does not function alone; CD151 is part of a tetraspanin-enriched microdomain (TEM) forming complexes that include proteins such as CD9 CD63 and other integrins. These complexes bring together structural and signaling molecules on the cell membrane creating platforms for efficient signal relay.

Pathways

CD151 plays a role in pathways involving cell adhesion and tissue repair. Notably it is involved in the integrin signaling pathway which is essential for cell-matrix interactions in tissues that require adhesion for repair or restructuring. CD151 interacts with proteins like PI3K and Akt within these pathways which are central to the signaling cascades regulating cellular responses to the extracellular environment.

Associated diseases and disorders

CD151 participation has been noted in cancer progression and metastasis. It links to the dysregulation observed in integrin functions such as those of integrin α3β1 which directly influences tumor cell invasion and metastasis in cancers like breast and prostate cancer. CD151 also exhibits connections with renal pathologies including Alport syndrome where the lack of functional basement membranes affects kidney function and it interacts with proteins like collagen IV in the disorder's progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

Western blot - Anti-CD151 antibody [EPR27423-31] (ab309512)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: Daudi(PMID:12705892), MOLT-4.

In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: 15 seconds
All lanes: Western blot - Anti-CD151 antibody [EPR27423-31] (ab309512) at 1/1000 dilution
Lane 1: HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: Daudi (human Burkitts lymphoma lymphoblast) whole cell lysate at 20 µg
Lane 3: MOLT-4 (human lymphoblastic leukemia T lymphoblast) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 28 kDa
Exposure time: 15s
Western blot - Anti-CD151 antibody [EPR27423-31] (ab309512)
Blocking and diluting buffer and concentration: 5% NFDM/TBST

Exposure time: 26 seconds
All lanes: Western blot - Anti-CD151 antibody [EPR27423-31] (ab309512) at 1/1000 dilution
Lane 1: Human kidney tissue lysate at 20 µg
Lane 2: Mouse kidney tissue lysate at 20 µg
Lane 3: Rat kidney tissue lysate at 20 µg
Lane 4: Human liver tissue lysate at 20 µg
Lane 5: Mouse liver tissue lysate at 20 µg
Lane 6: Rat liver tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 28 kDa
Exposure time: 26s
Western blot - Anti-CD151 antibody [EPR27423-31] (ab309512)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: EL4.IL-2(PMID: 25983660)

In Western blot, anti- H3 antibody (Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade ab176842) loading control staining at 1/100000 dilution.
Exposure time: 15 seconds
All lanes: Western blot - Anti-CD151 antibody [EPR27423-31] (ab309512) at 1/1000 dilution
Lane 1: bEnd.3 (mouse brain endothelial cell) while cell lysate at 20 µg
Lane 2: EL4.IL-2 (mouse lymphoma T lymphocyte) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 28 kDa
Exposure time: 15s
Immunocytochemistry/ Immunofluorescence - Anti-CD151 antibody [EPR27423-31] (ab309512)
Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized bEnd.3 (mouse brain endothelial cell) cells labelling CD151 with ab309512 at 1/50 (9.78 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing membranous staining in bEnd.3 cell line, and no staining in EL4.IL-2 cell line.Low expression: EL4.IL-2 (PMID: 25983660).Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.
Immunocytochemistry/ Immunofluorescence - Anti-CD151 antibody [EPR27423-31] (ab309512)
Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling CD151 with ab309512 at 1/50 (9.78 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing membranous staining in HepG2 cell line, and no staining in Daudi cell line.Low expression: Daudi (PMID:12705892).Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD151 antibody [EPR27423-31] (ab309512)
Immunohistochemical analysis of paraffin-embedded Mouse pancreatic can tissue labeling CD151 with ab309512 at 1/5000 (0.098 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on mouse pancreatic cancer . The section was incubated with ab309512 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD151 antibody [EPR27423-31] (ab309512)
Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling CD151 with ab309512 at 1/5000 (0.098 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on endothelium in rat liver . The section was incubated with ab309512 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD151 antibody [EPR27423-31] (ab309512)
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling CD151 with ab309512 at 1/5000 (0.098 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on endothelium in mouse liver . The section was incubated with ab309512 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD151 antibody [EPR27423-31] (ab309512)
Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue labeling CD151 with ab309512 at 1/2000 (0.244 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on human ovarian cancer. The section was incubated with ab309512 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD151 antibody [EPR27423-31] (ab309512)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling CD151 with ab309512 at 1/2000 (0.244 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on endothelium in human liver (PMID: 28473332). The section was incubated with ab309512 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins