Anti-CD151 antibody [EPR27423-31] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD151 antibody [EPR27423-31] - BSA and Azide free (AB309513)

This data was developed using ab309512, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue labeling CD151 with ab309512 at 1/2000 (0.244 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on human ovarian cancer. The section was incubated with ab309512 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD151 antibody [EPR27423-31] - BSA and Azide free (AB309513)

This data was developed using ab309512, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling CD151 with ab309512 at 1/2000 (0.244 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on endothelium in human liver (PMID : 28473332). The section was incubated with ab309512 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD151 antibody [EPR27423-31] - BSA and Azide free (AB309513)

This data was developed using ab309512, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling CD151 with ab309512 at 1/50 (9.78 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing membranous staining in HepG2 cell line, and no staining in Daudi cell line.Low expression : Daudi (PMID : 12705892).Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD151 antibody [EPR27423-31] - BSA and Azide free (AB309513)

This data was developed using ab309512, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized bEnd.3 (mouse brain endothelial cell) cells labelling CD151 with ab309512 at 1/50 (9.78 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing membranous staining in bEnd.3 cell line, and no staining in EL4.IL-2 cell line.Low expression : EL4.IL-2 (PMID : 25983660).Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD151 antibody [EPR27423-31] - BSA and Azide free (AB309513)

This data was developed using ab309512, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling CD151 with ab309512 at 1/5000 (0.098 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on endothelium in mouse liver . The section was incubated with ab309512 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD151 antibody [EPR27423-31] - BSA and Azide free (AB309513)

This data was developed using ab309512, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse pancreatic can tissue labeling CD151 with ab309512 at 1/5000 (0.098 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on mouse pancreatic cancer . The section was incubated with ab309512 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD151 antibody [EPR27423-31] - BSA and Azide free (AB309513)

This data was developed using ab309512, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling CD151 with ab309512 at 1/5000 (0.098 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on endothelium in rat liver . The section was incubated with ab309512 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-CD151 antibody [EPR27423-31] - BSA and Azide free (AB309513)

This data was developed using ab309512, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : Daudi(PMID : 12705892), MOLT-4. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 15 seconds

All lanes:

Western blot - Anti-CD151 antibody [EPR27423-31] (<a href='/en-us/products/primary-antibodies/cd151-antibody-epr27423-31-ab309512'>ab309512</a>) at 1/1000 dilution

Lane 1:

HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

Daudi (human Burkitts lymphoma lymphoblast) whole cell lysate at 20 µg

Lane 3:

MOLT-4 (human lymphoblastic leukemia T lymphoblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 28 kDa

false

Exposure time: 15s

• WB

Supplier Data

Western blot - Anti-CD151 antibody [EPR27423-31] - BSA and Azide free (AB309513)

This data was developed using ab309512, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Exposure time : 26 seconds

All lanes:

Western blot - Anti-CD151 antibody [EPR27423-31] (<a href='/en-us/products/primary-antibodies/cd151-antibody-epr27423-31-ab309512'>ab309512</a>) at 1/1000 dilution

Lane 1:

Human kidney tissue lysate at 20 µg

Lane 2:

Mouse kidney tissue lysate at 20 µg

Lane 3:

Rat kidney tissue lysate at 20 µg

Lane 4:

Human liver tissue lysate at 20 µg

Lane 5:

Mouse liver tissue lysate at 20 µg

Lane 6:

Rat liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 28 kDa

false

Exposure time: 26s

• WB

Supplier Data

Western blot - Anti-CD151 antibody [EPR27423-31] - BSA and Azide free (AB309513)

This data was developed using ab309512, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : EL4.IL-2(PMID : 25983660) In Western blot, anti- H3 antibody (ab176842) loading control staining at 1/100000 dilution. Exposure time : 15 seconds

All lanes:

Western blot - Anti-CD151 antibody [EPR27423-31] (<a href='/en-us/products/primary-antibodies/cd151-antibody-epr27423-31-ab309512'>ab309512</a>) at 1/1000 dilution

Lane 1:

bEnd.3 (mouse brain endothelial cell) while cell lysate at 20 µg

Lane 2:

EL4.IL-2 (mouse lymphoma T lymphocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 28 kDa

false

Exposure time: 15s