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AB276040

Anti-CD16 antibody [015] - BSA and Azide free

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Rabbit Recombinant Monoclonal FCGR3 antibody. Carrier free. Suitable for IP, WB and reacts with Mouse samples.

View Alternative Names

CD16, Low affinity immunoglobulin gamma Fc region receptor III, IgG Fc receptor III, Fc-gamma RIII, FcRIII, Fcgr3

2 Images
Immunoprecipitation - Anti-CD16 antibody [015] - BSA and Azide free (AB276040)
  • IP

Unknown

Immunoprecipitation - Anti-CD16 antibody [015] - BSA and Azide free (AB276040)

mFCGR3 was immunoprecipitated from0.5 mg RAW246.7 Whole Cell Lysate.
4μL ab276040 and 60 μg of Immunomagnetic beads Protein A/G.
Primary antibody : ab276040, at 1/100 dilution
Secondary antibody : Clean-Blot IP Detection Reagent (HRP) at 1/1000 dilution
Developed using the ECL technique.

Performed under reducing conditions.

All lanes:

Immunoprecipitation - Anti-CD16 antibody [015] - BSA and Azide free (ab276040)

false

Western blot - Anti-CD16 antibody [015] - BSA and Azide free (AB276040)
  • WB

Unknown

Western blot - Anti-CD16 antibody [015] - BSA and Azide free (AB276040)

Performed under reducing conditions.

All lanes:

Western blot - Anti-CD16 antibody [015] - BSA and Azide free (ab276040) at 1/500 dilution

All lanes:

C6 whole cell lysate at 30 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution

Predicted band size: 30 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

015

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

WB, IP

applications

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Complementary Products

Primary Antibodies

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 antibody (AB203883)

  • 0
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Primary Antibodies

AB246222

Anti-CD16 antibody [EPR22409-124]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 antibody [EPR22409-124] (AB246222)

  • 5
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Primary Antibodies

AB16702

Anti-CD23 antibody [SP23]

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Flow Cytometry - Anti-CD23 antibody [SP23] (AB16702)

  • 0
  • 0
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 16
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>0.5-2 μL/mg of lysate</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD16 also known as FCGR3A or CD16A protein is a low-affinity receptor for the Fc region of IgG. It weighs approximately 50-70 kDa and exists on the surface of several immune cells such as natural killer (NK) cells some populations of T cells and macrophages. The expression of CD16 mediates various cellular responses including antibody-dependent cellular cytotoxicity (ADCC) where it acts by engaging with immune complexes. Researchers often use anti-CD16 antibodies in flow cytometry to analyze the presence and quantity of CD16-expressing cells.
Biological function summary

This receptor plays a significant role in immune system regulation by contributing to the activation of immune effector cells. CD16 is not part of a larger complex but it operates in concert with CD3 proteins to trigger intracellular signaling pathways upon engagement with its ligand. Its function is important for the antibody-dependent clearance of infected or malignant cells from the body representing an essential linkage between innate and adaptive immunity.

Pathways

Signaling involving CD16 is integrated into the immune response mechanisms that include the Fc gamma receptor-mediated phagocytosis pathway. This pathway involves interactions with IgG antibodies and complements the activity of other Fc gamma receptors such as CD32 and CD64. Through these interactions CD16 enhances phagocytic activity and stimulates the secretion of cytokines and chemokines important for orchestrating an effective immune response.

CD16 plays an important role in conditions like autoimmune diseases and certain cancers. Its expression and function are important in the pathophysiology of systemic lupus erythematosus where an imbalance in immune complex clearance leads to tissue damage. In oncology CD16 aids in the immune system's recognition and elimination of tumor cells working alongside proteins such as CD3 in modulating the body's anti-tumor response. Understanding CD16's involvement in these diseases helps in developing therapeutic strategies that harness the immune system's power such as monoclonal antibody therapies targeting CD16.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for the Fc region of complexed immunoglobulins gamma. Low affinity receptor which binds to IgG1, IgG2a and IgG2b (PubMed : 17558411). Mediates neutrophil activation by IgG complexes redundantly with Fcgr4 (PubMed : 18097064).
See full target information Fcgr3
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Product promise

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