Rabbit Polyclonal CD16a antibody. Suitable for Flow Cyt, IHC-P and reacts with Human, Mouse samples. Cited in 17 publications. Immunogen corresponding to Synthetic Peptide within Human FCGR3A aa 100-200 conjugated to Keyhole Limpet Haemocyanin.
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- Neuropathology : official journal of the Japanese Society of Neuropathology 42:181-1892022NLRP3 inflammasome inhibitor ameliorates ischemic stroke by reprogramming the phenotype of microglia/macrophage in a murine model of distal middle cerebral artery occlusion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongning Jia,Xiaoyuan Qi,Lan Fu,Huijun Wu,Jinxing Shang,Mingwei Qu,Chaoping Yang,Jianping WangPubMed 35434787
- Archives of rheumatology 37:335-3432022CD14+ monocytes and soluble CD14 of synovial fluid are associated with osteoarthritis progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHa-Reum Lee,Sunyoung Lee,In Seol Yoo,Su-Jin Yoo,Mi-Hye Kwon,Chung-Il Joung,Ji Ah Park,Seong Wook Kang,Jinhyun KimPubMed 36589618
- The Journal of clinical investigation 132:2022Lysosomal exocytosis of HSP70 stimulates monocytic BMP6 expression in Sjögren's syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing-Qian Mo,Hiroyuki Nakamura,Tsutomu Tanaka,Toshio Odani,Paola Perez,Youngmi Ji,Benjamin N French,Thomas Jf Pranzatelli,Drew G Michael,Hongen Yin,Susan S Chow,Maryam Khalaj,Sandra A Afione,Changyu Zheng,Fabiola Reis Oliveira,Ana Carolina F Motta,Alfredo Ribeiro-Silva,Eduardo M Rocha,Cuong Q Nguyen,Masayuki Noguchi,Tatsuya Atsumi,Blake M Warner,John A ChioriniPubMed 35113815
- International journal of chronic obstructive pulmonary disease 16:3575-35912021Spiperone Stimulates Regeneration in Pulmonary Endothelium Damaged by Cigarette Smoke and Lipopolysaccharide.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEvgenii Skurikhin,Olga Pershina,Mariia Zhukova,Darius Widera,Edgar Pan,Angelina Pakhomova,Vyacheslav Krupin,Natalia Ermakova,Victoria Skurikhina,Lubov Sandrikina,Sergey Morozov,Aslan Kubatiev,Alexander DygaiPubMed 35002229
- BMC complementary medicine and therapies 21:1502021Homogeneous polyporus polysaccharide inhibits bladder cancer by polarizing macrophages to M1 subtype in tumor microenvironment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenyu Jia,Siwan Luo,Gena Lai,Shiqi Li,Shuai Huo,Meifang Li,Xing ZengPubMed 34034714
- Aging 12:2021Inhibition of Notch1-mediated inflammation by intermedin protects against abdominal aortic aneurysm via PI3K/Akt signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXian-Qiang Ni,Ya-Rong Zhang,Li-Xin Jia,Wei-Wei Lu,Qing Zhu,Jin-Ling Ren,Yao Chen,Lin-Shuang Zhang,Xin Liu,Yan-Rong Yu,Mo-Zhi Jia,Zhong-Ping Ning,Jie Du,Chao-Shu Tang,Yong-Fen QiPubMed 33535178
- BMC complementary medicine and therapies 21:62021Germacrone alleviates neurological deficits following traumatic brain injury by modulating neuroinflammation and oxidative stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSujing Zhuang et. al.PubMed 33402180
- BioMed research international 2020:80356712020Kupffer Cell-Derived TNF- Triggers the Apoptosis of Hepatic Stellate Cells through TNF-R1/Caspase 8 due to ER Stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei-Min Wang et. al.PubMed 32802876
- Journal of neuroinflammation 17:1342020LncGBP9/miR-34a axis drives macrophages toward a phenotype conducive for spinal cord injury repair via STAT1/STAT6 and SOCS3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiahui Zhou et. al.PubMed 32345320
- Journal of Cancer 11:4250-42602020IL-2 enhanced MHC class I expression in papillary thyroid cancer with Hashimoto's thyroiditis overcomes immune escape .Applications:Unspecified applicationReactive species:Unspecified reactive speciesJia-Qian Hu et. al.PubMed 32368308
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human FCGR3A aa 100-200 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P08637
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Reactivity data
Select an application| Flow Cyt | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20 - 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 - 1/500 | Notes When using a fluorescent probe the recommended dilution is 1/50 – 1/200. |
Species Human | Dilution info 1/100 - 1/500 | Notes When using a fluorescent probe the recommended dilution is 1/50 – 1/200. |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.
Alternative names
Low affinity immunoglobulin gamma Fc region receptor III-A, CD16a antigen, Fc-gamma RIII-alpha, FcR-10, IgG Fc receptor III-2, Fc-gamma RIII, Fc-gamma RIIIa, FcRIII, FcRIIIa, FCGR3A, FCGR3, FCG3, IGFR3, CD16A
Recommended products
Rabbit Polyclonal CD16a antibody. Suitable for Flow Cyt, IHC-P and reacts with Human, Mouse samples. Cited in 17 publications. Immunogen corresponding to Synthetic Peptide within Human FCGR3A aa 100-200 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human FCGR3A aa 100-200 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P08637
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD16 also known as FCGR3A or CD16A protein is a low-affinity receptor for the Fc region of IgG. It weighs approximately 50-70 kDa and exists on the surface of several immune cells such as natural killer (NK) cells some populations of T cells and macrophages. The expression of CD16 mediates various cellular responses including antibody-dependent cellular cytotoxicity (ADCC) where it acts by engaging with immune complexes. Researchers often use anti-CD16 antibodies in flow cytometry to analyze the presence and quantity of CD16-expressing cells.
- Biological function summary
This receptor plays a significant role in immune system regulation by contributing to the activation of immune effector cells. CD16 is not part of a larger complex but it operates in concert with CD3 proteins to trigger intracellular signaling pathways upon engagement with its ligand. Its function is important for the antibody-dependent clearance of infected or malignant cells from the body representing an essential linkage between innate and adaptive immunity.
- Pathways
Signaling involving CD16 is integrated into the immune response mechanisms that include the Fc gamma receptor-mediated phagocytosis pathway. This pathway involves interactions with IgG antibodies and complements the activity of other Fc gamma receptors such as CD32 and CD64. Through these interactions CD16 enhances phagocytic activity and stimulates the secretion of cytokines and chemokines important for orchestrating an effective immune response.
- Associated diseases and disorders
CD16 plays an important role in conditions like autoimmune diseases and certain cancers. Its expression and function are important in the pathophysiology of systemic lupus erythematosus where an imbalance in immune complex clearance leads to tissue damage. In oncology CD16 aids in the immune system's recognition and elimination of tumor cells working alongside proteins such as CD3 in modulating the body's anti-tumor response. Understanding CD16's involvement in these diseases helps in developing therapeutic strategies that harness the immune system's power such as monoclonal antibody therapies targeting CD16.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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4 product images
Flow Cytometry - Anti-CD16 antibody (ab203883)
U937 cells probed with ab203883 at a 1/100 for 40 minutes followed by incubation with Goat Anti-Rabbit IgG PE conjugated secondary at 1/100 (green) for 40 minutes compared to control cells (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 antibody (ab203883)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human glioma tissue labeling CD16 with ab203883 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 antibody (ab203883)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lung cancer tissue labeling CD16 with ab203883 at 1/200 dilution followed by Goat Anti-Rabbit IgG, Cy3 conjugated secondary antibody at 1/200 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 antibody (ab203883)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse spleen tissue labeling CD16 with ab203883 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
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